Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design     

Leticia I. RAMíREZ-CAVAZOS  Charles JUNGHANNS  Rakesh NAIR  Diana L. CáRDENAS-CHáVEZ  Carlos HERNáNDEZ-LUNA  Spiros N. AGATHOS  Roberto PARRA 《Journal of Zhejiang University. Science. B》2014,15(4):343-352

研究目的：优化获得血红密孔菌（P.sanguineus）的最佳培养基组成,提高耐热漆酶的产量。创新要点：获得了目前文献报道的最高水平的漆酶活力。研究方法：通过单因素试验研究了不同培养基（番茄汁、麦麸、麦芽提取物和葡萄糖细菌蛋白胨培养基）和不同组合诱导剂（大豆油、阿魏酸、没食子酸、二甲基苯胺、酸性蓝62和活性蓝19分别与硫酸铜组合诱导剂）对P.sanguineus产耐热漆酶的影响。在此基础上采用中心组合试验设计,进一步研究了番茄汁培养基结合硫酸铜和大豆油组合诱导剂对P.sanguineus产耐热漆酶的影响。利用SAS10.0和响应面分析方法对试验结果进行了统计分析和建立回归模型。重要结论：通过中心组合设计优化得出P.sanguineus产耐热漆酶的最优培养基条件：以36.8%番茄汁为培养基,以3 mmol/L硫酸铜和1%大豆油作为组合诱导剂。该条件下在10 L搅拌槽生物反应器中漆酶活力达到了143 000 IU/L（2,2＇-联氮双（3-乙基苯并噻唑啉-6-磺酸）为底物,pH值为3.0）。  相似文献   

Physical activity recommendations and cardiovascular disease risk factors in young Hispanic women     

Vella CA  Ontiveros D  Zubia RY  Dalleck L 《Journal of sports sciences》2011,29(1):37-45

Despite the benefits associated with regular physical activity, there is little epidemiological evidence to support positive health outcomes when meeting physical activity guidelines in high-risk ethnic groups, such as Hispanic women. We compared cardiovascular disease risk factors between young Hispanic women who meet and those who do not meet current physical activity guidelines. Height, weight, waist circumference, and blood pressure were measured in 60 Hispanic women aged 20-39 years. Lipids, C-reactive protein, insulin, and glucose were assessed. Body composition and cardiovascular fitness were assessed by BodPod and maximal oxygen uptake (VO?(max)) respectively. Participants wore an accelerometer and average minutes (assessed in 10-min bouts) spent in light, moderate, and hard daily activity for weekdays and weekends was determined. Seventy percent of participants did not meet the recommended physical activity guidelines, whereas 30% did so. Following current physical activity guidelines was associated with significantly lower mean cholesterol (mean ± s: 4.2 ± 0.8 vs. 4.7 ± 0.9 mmol · l?1) and triglycerides (0.7 ± 0.3 vs. 1.1 ± 0.6 mmol · l?1), and higher fat-free mass (43.3 ± 3.8 vs. 40.2 ± 5.1 kg) and relative (40.4 ± 7.6 vs. 35.6 ± 7.0 ml · kg?1 · min?1) and absolute (2.5 ± 0.3 vs. 2.1 ± 0.4 litres · min?1) VO?(max) (P < 0.05). These findings suggest an improved health status in women who meet versus those who did not meet current physical activity guidelines.  相似文献   

Implementation fidelity of a program designed to promote personal and social responsibility through physical education: a comparative case study     

Pascual C  Escartí A  Llopis R  Gutíerrez M  Marín D  Wright PM 《Research quarterly for exercise and sport》2011,82(3):499-511

The purpose of this qualitative comparative case study was to examine the implementation fidelity of a program designed to deliver the Teaching Personal and Social Responsibility (TPSR) model (Hellison, 2003) through physical education and its relationship with short-term outcomes for elementary school students. The research questions were: (a) was the program implemented with fidelity, and (b) did better fidelity yield better student outcomes. Thus, we conducted a study on the implementation process used by two teachers who delivered the same program in two physical education classes in two different elementary schools in Spain. Data sources included observations and interviews with teachers and nonparticipant observers. Findings indicated that fidelity of implementation in Case 1 was higher and most children in those classes acquired the first three of five TPSR responsibility levels. Implementation fidelity in Case 2 was weaker and achievement of responsibility goals was minimal (only the first of five levels) and less stable for those students. This study is the first to directly examine the connection between TPSR implementation fidelity and student outcomes.  相似文献   

The Needs of the Left-handed Child in the Infant Classroom – Writing is not always Right     

Diana Bentley  Rhona Stainthorp 《Literacy》1993,27(2):4-8

  相似文献   

The Latin American Fashion Reader     

Diana Risk 《Journal of American culture (Malden, Mass. : 2003)》2006,29(2):255-257

  相似文献   

Mock Interdisciplinary Staffing: Educating for Interprofessional Collaboration     

Diana Quealy‐Berge  Karen Caldwell 《Counselor Education & Supervision》2004,43(4):310-320

Training for interprofessional collaboration is important because professionals are increasingly required to work together in a coordinated and collaborative manner to meet the complex needs of clients. However, few published reports exist on interprofessional training for community counselors and marriage and family therapists. The authors discuss the use of a mock interdisciplinary staffing with students who are training to be counselors and marriage and family therapists. This method of instruction holds promise as an effective means of introducing students to a range of issues, including the knowledge, skills, and attitudes needed for interprofessional practice.  相似文献   

Mediating the message: television programme design and students' understanding     

Diana Laurillard 《Instructional Science》1991,20(1):3-23

The research reported represents a non-standard approach to basic research on educational television, in that it uses qualitative methods to describe learning and to illuminate which programme features are important. It was based on observation, interview and test data from five groups of 20–30 Open University students, each watching a social science television programme as part of their course. A qualitative analysis of students' summaries of the programmes showed that only half achieved the intended learning outcomes. A further analysis of the students' responses to key extracts showed that this is more likely to be because of programme structure than because of presentational quality. The research design and results are related to similar studies on students learning from text, and contrasted with the methodology adopted in other studies of the effects of educational television.  相似文献   

The Challenge and Opportunity of Competency‐Based Counselor Education     

Patrick Akos  Suzan Z. Wasik  Angela McDonald  Michelle Soler  Diana Lys 《Counselor Education & Supervision》2019,58(2):98-111

There is increased responsibility for programs to demonstrate evidence of student learning and skill. Application of competency‐based education is delineated, including prior learning assessment and personalized learning. Implications such as awarding credit for experience in admissions or variable clinical training timelines and requirements are explored.  相似文献   

Assessment revisited: a review of research in Assessment and Evaluation in Higher Education     

Diana Pereira  Maria Assunção Flores  Laila Niklasson 《Assessment & Evaluation in Higher Education》2016,41(7):1008-1032

A review of articles published in Assessment and Evaluation in Higher Education, over the last eight years (2006–2013) on assessment in higher education, since the introduction of the Bologna process, is the subject of the paper. The first part discusses the key issue of assessment in higher education and the method used for selecting articles. The second part presents results according to the main emerging themes arising from data analysis: assessment methods, modes of assessment and assessment related to a given teaching and learning method. The paper concludes that the foci of the studies are aligned with assessment practices other than the written test, in accordance with a learner-oriented perspective. Although the implementation of the Bologna process has had different kinds of impact in different European countries, the review shows that the use and effects of a diversity of assessment methods in higher education have been investigated, particularly those pointing to the so-called alternative methods. Implications of the findings are discussed.  相似文献   

Educating women about HIV/AIDS: some international comparisons     

Donna Bulman  Diana Coben  Nguyen Van Anh 《Compare》2004,34(2):141-159

This paper describes current trends in the HIV/AIDS epidemic. It looks at issues and strategies involved in educating women about HIV/AIDS in the context of the global pandemic, focusing particularly on Canada and Vietnam. These strategies are essential steps in preventing the spread of HIV and in caring for those who have already developed AIDS. The strategies identified include the formulation of public policy within Canada, Vietnam, and the UK which would strive to eliminate inequality and discrimination, while at the same time positioning HIV/AIDS education within a gender‐sensitive perspective. Other educational strategies include teaching women how to practise negotiated safety while understanding the power dynamics within which safer sex must occur, being sensitive to local cultures and moving away from individualistic programmes towards programmes that focus on structural inequalities.  相似文献