Pulmonary monoclonal antibody delivery via a portable microfluidic nebulization platform     

Christina Cortez-Jugo  Aisha Qi  Anushi Rajapaksa  James R. Friend  Leslie Y. Yeo 《Biomicrofluidics》2015,9(5)

Nebulizers have considerable advantages over conventional inhalers for pulmonary drug administration, particularly because they do not require coordinated breath actuation to generate and deliver the aerosols. Nevertheless, besides being less amenable to miniaturization and hence portability, some nebulizers are prone to denature macromolecular drugs due to the large forces generated during aerosolization. Here, we demonstrate a novel portable acoustomicrofluidic device capable of nebulizing epidermal growth factor receptor (EGFR) monoclonal antibodies into a fine aerosol mist with a mass median aerodynamic diameter of approximately 1.1 μm, optimal for deep lung deposition via inhalation. The nebulized monoclonal antibodies were tested for their stability, immunoactivity, and pharmacological properties, which confirmed that nebulization did not cause significant degradation of the antibody. In particular, flow cytometry demonstrated that the antigen binding capability of the antibody is retained and able to reduce phosphorylation in cells overexpressing the EGFR, indicating that the aerosols generated by the device were loaded with stable and active monoclonal antibodies. The delivery of antibodies via inhalation, particularly for the treatment of lung cancer, is thus expected to enhance the efficacy of this protein therapeutic by increasing the local concentration where they are needed.  相似文献   

FRESHMAN ENGLISH IN AMERICA     

MARTIN STEINMAN JR 《Higher Education Quarterly》1965,19(4):391-395

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PROGRESSIVE EDUCATION: A CASE OF ARRESTED DEVELOPMENT? A Brief Critical Review of Twentieth Century Educational Theory     

HENRY C. JOHNSON  JR. 《Educational theory》1965,15(3):188-197

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World Hypotheses: A Basis For A Structural Curriculum     

JAMES H. QUINA JR. 《Educational theory》1971,21(3):311-319

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DEWEY'S CONSISTENT ATTITUDE TOWARD HISTORY     

ERNEST H. JUSTIN  JR. 《Educational theory》1965,15(3):198-204

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Microfluidic blood plasma separation via bulk electrohydrodynamic flows   总被引：1，自引：0，他引：1  

Dian R. Arifin  Leslie Y. Yeo    James R. Friend 《Biomicrofluidics》2007,1(1)

An effective mechanism for rapid and efficient microfluidic particle trapping and concentration is proposed without requiring any mechanically moving parts. When a voltage beyond the threshold atmospheric ionization value is applied on a sharp electrode tip mounted at an angle above a microfluidic liquid chamber, the bulk electrohydrodynamic air thrust that is generated results in interfacial shear and, hence, primary azimuthal liquid surface recirculation. This discharge driven vortex mechanism, in turn, causes a secondary bulk meridional liquid recirculation, which produces an inward radial force near the bottom of the chamber. Particles suspended in the liquid are then rapidly convected by the bulk recirculation toward the bottom, where the inward radial force causes them to spiral in a helical swirl-like fashion toward a stagnation point. In particular, we show that these flows, similar to Batchelor flows occurring in a cylindrical liquid column between a stationary and rotating disk, can be used for the separation of red blood cells from blood plasma in a miniaturized device.  相似文献   

What is education for? An anthology on education     

Anthony D. Friend 《Educational Philosophy and Theory》2018,50(10):998-999

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Sweat lactate response during cycling at 30°C and 18°C WBGT     

JM Green  RC Pritchett  DC Tucker  TR Crews  JR McLester 《Journal of sports sciences》2013,31(4):321-327

Sweat lactate reflects eccrine gland metabolism. However, the metabolic tendencies of eccrine glands in a hot versus thermoneutral environment are not well understood. Sixteen male volunteers completed a maximal cycling trial and two 60-min cycling trials [30°C?=?30±1°C and 18°C?=?18±1°C wet bulb globe temperature (WBGT)]. The participants were requested to maintain a cadence of 60 rev?·?min^?1 with the intensity individualized at ～ 90% of the ventilatory threshold. Sweat samples at 10, 20, 30, 40, 50 and 60?min were analysed for lactate concentration. Sweat rate at 30°C (1380±325?ml?·?h^?1) was significantly greater (P<0.05) than at 18°C (632±311?ml?·?h^?1). Sweat lactate concentration was significantly greater (P<0.05) at each time point during the 18°C trial, with values between trials tending to converge across time. During the 30°C trial, both heart rate (20, 30, 40, 50 and 60?min) and rectal temperature (30, 40, 50 and 60?min) were significantly higher than in the 18°C trial. Higher sweat lactate concentrations coupled with lower sweat rates may indicate a greater relative contribution of oxygen-independent metabolism within eccrine glands during exercise at 18°C. Decreases in sweat lactate concentration across time suggest either greater dilution due to greater sweat volume or increased reliance on aerobic metabolism within eccrine glands. The convergence of lactate concentrations between trials may indicate that time-dependent modifications in sweat gland metabolism occur at different rates contingent partially on environmental conditions.  相似文献   

TIME EXPOSURE: African American Rural Schools at the Time of the First World War     

EUGENE F. PROVENZO  JR. 《Educational Studies A Journal of the American Educational Studies Association》2013,49(3):332-333

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Book Review Section 2     

HENRIETTA SCHWARTZ  RONALD D. COHEN  JAMES J. SHIELDS  JR.  MAZOOR AHMED  ALBERT E. BENDER  PAUL J. SCHAFER 《Educational Studies A Journal of the American Educational Studies Association》2013,49(3-4):71-84

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