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Ri-sheng Que Cheng Lin Guo-ping Ding Zheng-rong Wu Li-ping Cao 《Journal of Zhejiang University. Science. B》2016,17(5):352-360
Background
Tumor-derived exosomes were considered to be potential candidates for tumor vaccines because they are abundant in immune-regulating proteins, whereas tumor exosomal miRNAs may induce immune tolerance, thereby having an opposite immune function.Objective
This study was designed to separate exosomal protein and depleted exosomal microRNAs (miRNAs), increasing the immune activity of exosomes for activating dendritic cell/cytokine-induced killer cells (DC/CIKs) against pancreatic cancer (PC).Methods
PC-derived exosomes (PEs) were extracted from cultured PANC-1 cell supernatants and then ruptured; this was followed by ultrafiltered exosome lysates (UELs). DCs were stimulated with lipopolysaccharide (LPS), PE, and UEL, followed by co-culture with CIKs. The anti-tumor effects of DC/CIKs against PC were evaluated by proliferation and killing rates, tumor necrosis factor-α (TNF-α) and perforin secretion. Exosomal miRNAs were depleted after lysis and ultrafiltration, while 128 proteins were retained, including several immune-activating proteins.Results
UEL-stimulated DC/CIKs showed a higher killing rate than LPS- and PE-stimulated DC/CIKs.Conclusions
miRNA-depleted exosome proteins may be promising agonists for specifically activating DC/CIKs against PC.43.
Shen?Da-keEmail author Han?Gao-rong Du?Pi-yi Que?Duan-lin Sou?I.?K. 《浙江大学学报(A卷英文版)》2004,5(2):212-217
The structural characteristics and optical and electrical properties of molecular-beam-epitaxy (MBE) grown ZnS0.8Se0.2 thin films on indium-tin-oxide(ITO) glas substrates were investigated in this work. TheX-ray diffraction (XRD) results indicated that high quality polycrystalline ZnS0.8Se0.2 thin film grown at the optimized temperature had a prefered orientation along the (111) planes. The transmission electron
microscopy (TEM) cross-sectional micrograph of the sample showed a well defined columnar structure with lateral crystal dimension
in the order of a few hundred angstroms. Ultraviolet (UV) photoresponsivity as high as 0.01 A/W had been demonstrated and
for wavelengths longer than 450 nm, the response was down from the peak response by more than 3 orders of magnitude. The thin
ZnS0.8Se0.2 photosensor layer, with a wide energy gap and anisotropic electrical property, makes a transmission UV liquid crystal light
valve (LCLV) with high resolution feasible.
Project supported by the National Natural Science Foundation of China (No. 59910161981) and RGC grant from the Hong Kong Government
Grant (No. NSFC/HKUST 35), China 相似文献
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INTRODUCTION Ensuring the integrity and safe operation of oil pipelines network is critical for ensuring the trans- portation of one of the most important forms of en- ergy. An inspection vehicle, called intelligent pig, which employs ultrasonic non-destructive testing technique is often used to inspect oil transmission pipelines (Okamoto et al., 1999; Reher et al., 2002). The ultrasonic detection principle involves the fun- damental concept that a propagating wave will be reflected and/o… 相似文献
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Guo-ping Ding Li-ping Cao Da-ren Liu Ri-sheng Que 《Journal of Zhejiang University. Science. B》2012,13(3):186-191
Objective
To evaluate the feasibility and superiority of a new coagulating and hemostatic method named “saline conducted electric coagulation (SCEC)”. 相似文献46.
The professional supervision of new graduates to ensure both their effectiveness with clients and their personal learning and development is a common feature of a range of human services contexts. This study investigated psychology supervisees’ perceptions of relationship processes and outcomes in professional supervision. The relationship constructs of supervisor support, challenge and openness were investigated and related to the outcome variables of supervisee anxiety and perceived effectiveness of supervision. Psychology graduates (n=261) involved in the process of professional supervision for registration responded to a mail survey regarding the quality of their supervisory relationship. Findings established the relationship dimensions of levels of supervisor challenge, supervisor support and supervisor openness as independent but related constructs. Supervisees’ perceptions of supervisor support and openness predicted their perceptions of supervisor effectiveness. Supervisees’ perceptions of level of supervisor challenge predicted their self-reported levels of evaluative anxiety or defensiveness in the supervisory process. 相似文献
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阙宁南 《图书馆工作与研究》2012,(7):104-106
本文针对数字化环境中用户需求的新变化及信息服务的新特点,探讨了在数字化环境中图书馆信息服务的发展创新,重点阐述了从服务内容和服务形式上的发展创新,以期提高数字化环境中图书馆信息服务的质量与水平。 相似文献
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影子价格是为实现一定的经济发展目标而确定的、比市场价格更能反映出资源真实价值、能促进合理利用资源的经济价格。结合实例阐述了如何运用影子价格理论指导企业经营管理,从而提高企业的经济效益。 相似文献
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运动员攻击性行为的问题分析 总被引：1，自引：0，他引：1
功利因素的刺激，传媒的渲染及众多球迷的参与，使比赛带有鲜明浓厚的政治性、民族性与商业性。尤其是一些带有身体接触的运动项目，运动员会发生直接的冲突，引发斗殴等攻击与暴力性行为。通过对运动员攻击性行为的特征、攻击性行为的分类、表现与区别等进行阐述，找明原因，分析心理。 相似文献
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对第六届全国城市运动会篮球比赛参赛运动员的投入与收益进行了调查与分析,通过与不同学历的劳动者的比较,得出篮球运动员与接受全日制教育的普通人相比存在较大机会成本的结论,建议通过加强篮球运动员在训期间的文化教育以降低机会成本。 相似文献