排序方式： 共有66条查询结果，搜索用时 15 毫秒
31.
Guillaume Mottet Karla Perez-Toralla Ezgi Tulukcuoglu Francois-Clement Bidard Jean-Yves Pierga Irena Draskovic Arturo Londono-Vallejo Stephanie Descroix Laurent Malaquin Jean Louis Viovy 《Biomicrofluidics》2014,8(2)
We present a low cost microfluidic chip integrating 3D micro-chambers for the capture and the
analysis of cells. This device has a simple design and a small footprint. It allows the
implementation of standard biological protocols in a chip format with low volume consumption. The
manufacturing process relies on hot-embossing of cyclo olefin copolymer, allowing the development of
a low cost and robust device. A 3D design of microchannels was used to induce high flow velocity
contrasts in the device and provide a selective immobilization. In narrow distribution channels, the
liquid velocity induces a shear stress that overcomes adhesion forces and prevents cell
immobilization or clogging. In large 3D chambers, the liquid velocity drops down below the threshold
for cell attachment. The devices can be operated in a large range of input pressures and can even be
handled manually using simple syringe or micropipette. Even at high flow injection rates, the 3D
structures protect the captured cell from shear stress. To validate the performances of our device,
we implemented immuno-fluorescence labeling and Fluorescence in Situ Hybridization
(FISH) analysis on cancer cell lines and on a patient pleural effusion sample. FISH is a Food and
Drug Administration approved cancer diagnostic technique that provides quantitative information
about gene and chromosome aberration at the single cell level. It is usually considered as a long
and fastidious test in medical diagnosis. This process can be easily implanted in our platform, and
high resolution fluorescence imaging can be performed with reduced time and computer intensiveness.
These results demonstrate the potential of this chip as a low cost, robust, and versatile tool
adapted to complex and demanding protocols for medical diagnosis. 相似文献
32.
Beyond the Screen: Narrative Mapping as a Tool for Evaluating a Mixed-Reality Science Museum Exhibit
Karla Saari Kitalong Jane E. Moody PhD Rebecca Helminen Middlebrook Gary Saldana Ancheta 《Technical Communication Quarterly》2013,22(2):142-165
This article describes the authors' work as formative evaluators of a mixed-reality science museum installation, Journey with Sea Creatures. Looking beyond the focal point of the screen to the spatial and temporal surroundings of the exhibit, the authors employed a technique they call retrospective narrative mapping in conjunction with sustained on-site observations, follow-up interviews with museum visitors, and the development of personas to better understand the user experience in multimodal informal learning environments. 相似文献
33.
This study identifies factors associated with freshmen retention rates at a mid‐size private university. A key finding from our analysis is that roommates' academic efforts, residing on an honours floor and membership in co‐curricular groups increase retention. We also confirm many standard results in the literature regarding the impact on retention rates, such as student motivation and ability, and financial aid. We show that a timely housing application, closer proximity to home, a higher number of schools on a student's Free Application for Federal Student Aid and peer effects impact freshman retention. Our results suggest that a more sophisticated dormroom assignment algorithm matching students' abilities and interests could be considered to increase freshman retention rates among private institutions. 相似文献
34.
Research in Science Education - Informal formative assessments (IFAs) are classroom interactions teachers use to gather information about their students’ learning, interpret it, and act on... 相似文献
35.
Interviews with 14 technical communicators reveal that skills in rhetorical invention help them creatively address communication problems. They define creativity in relation to four interrelated exigencies of invention: thinking like a user, reinvigorating dry content, inventing visual ideas, and alternating between heuristic and algorithmic processes. They recognize intrinsic factors such as curiosity and sympathy as motivations for their creativity, while being conscious of the external factors (people, money, and time) that may restrain creativity. 相似文献
36.
Yolanda Salinas-Alvarez Alejandro Quiroga-Garza Javier Humberto Martinez-Garza Guillermo Jacobo-Baca Pablo Patricio Zarate-Garza Karla Valeria Rodríguez-Alanís Santos Guzman-Lopez Rodrigo Enrique Elizondo-Omaña 《Anatomical sciences education》2021,14(4):471-481
Anatomical sciences curricula have been under constant reform over the years, with many countries having to reduce course hours while trying to preserve laboratory time. In Mexico, schools have historically been autonomous and unregulated, and data regarding structure and methods are still lacking. A national survey was sent by the Mexican Society of Anatomy to 110 anatomical sciences educators. The questionnaire consisted of 50 items (open and multiple choice) for gross anatomy, microscopic anatomy, neuroanatomy, and embryology courses in medical schools across Mexico. A clinical approach was the most common course approach in all disciplines. Contact course hours and laboratory hours were higher in Mexican anatomy education compared to other countries, with the highest reported contact hours for embryology (133.4 ± 44.1) and histology (125 ± 33.2). There were similar contact hours to other countries for gross anatomy (228.5 ± 60.5). Neuroanatomy course hours (43.9 ± 13.1) were less than reported by the United States and similar to Saudi Arabia and higher than the United Kingdom. Dissection and microscopy with histological slides predominate as the most common laboratory activities. Traditional methods prevail in most of the courses in Mexico and only a few educators have implemented innovative and technological tools. Implementation of new methods, approaches, and curricular changes are needed to enhance anatomical sciences education in Mexico. 相似文献
37.
Stefanie A. Nelemans Loes Keijsers Hilde Colpin Karla van Leeuwen Patricia Bijttebier Karine Verschueren Luc Goossens 《Child development》2020,91(3):814-828
This 4-year longitudinal multi-informant study examined between- and within-person associations between adolescent social anxiety symptoms and parenting (parental psychological control and autonomy support). A community sample of 819 adolescents (46.1% girls; MageT1 = 13.4 years) reported annually on social anxiety symptoms and both adolescents and mothers reported on parenting. Between-person associations suggested that adolescent social anxiety symptoms were associated with higher adolescent- and mother-reported psychological control and lower mother-reported autonomy support. At the within-person level, however, mothers reported lower psychological control and higher autonomy support after periods with higher adolescent social anxiety symptoms. Our findings illustrate the importance of distinguishing among between-person and within-person associations and including perceptions of both dyad members in longitudinal research concerning parenting and adolescent mental health. 相似文献
38.
It has been seen that children's scores on reading achievement tests vary not only with knowledge of content, but also with the differing formats of test items. Teachers working with learning disabled children or children with attention problems may wish to choose standardized tests with fewer, rather than more, format changes. The present study evaluated the number of format and direction changes across tests and grade levels of the major elementary standardized reading achievement tests. The number of format changes varies from one change every 1.2 minutes on the Metropolitan Achievement Test Level E1 to one change every 21.3 minutes on the P1 level of the Stanford Achievement Test. Teachers may wish to take this evaluation into account when considering use of standardized reading achievement tests for their students. 相似文献
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Visual input during development seems crucial in tactile spatial perception, given that late, but not congenitally, blind people are impaired when skin‐based and tactile external representations are in conflict (when crossing the limbs). To test whether there is a sensitive period during which visual input is necessary, 14 children (age = 7.95) and a teenager (LM; age = 17.38) deprived of early vision by cataracts, and whose sight was restored during the first 5 months and at age 7, respectively, were tested. Tactile localization with arms crossed and uncrossed was measured. Children showed a crossing effect indistinguishable from a control group (Ns = 28, age = 8.24), whereas LM showed no crossing effect (Ns controls = 14, age = 20.78). This demonstrates a sensitive period which, critically, does not include early infancy. 相似文献