Clones identification of Sequoia sempervirens (D.Don) Endl.in Chile by using PCR-RAPDs technique     

Manuel TORAL IBAEZ  Margarita CARU  Miguel A.HERRERA  Luis GONZALEZ  Luis M.MARTIN  Jorge MIRANDA  Rafael M.NAVARRO-CERRILLO 《Journal of Zhejiang University. Science. B》2009,10(2)

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D.Don) Endl.in Chile.Ten (out of 34) clones from introduction trial located in Voipir-Viilarrica,Chile,were studied.The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.The PCR tests were carried out employing 10-mer random primers.The amplification products were detected by electrophoresis in agarose gels.Forty nine polymorphic bands were obtained with the selected primers (BG04,BF07,BF 12,BF13,and BF 14) and were ordered according to their molecular size.The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).Of the primers tested,5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism.A total of 49 polymorphic RAPD bands were detected out of 252 bands.The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones,suggesting a geographic relationship.The results indicate that the RAPD markers permitted the identification of the assayed clones,although they are derived from the same geographic origin.  相似文献   

Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique     

Manuel Toral Ibañez  Margarita Caru  Miguel A. Herrera  Luis Gonzalez  Luis M. Martin  Jorge Miranda  Rafael M. Navarro-Cerrillo 《Journal of Zhejiang University. Science. B》2009,10(2):112-119

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geographic origin. Project (No. CONICYT-FONDEF PROYECT D01 I 1008) supported by the National Commission of Science and Research of Chile  相似文献   

The condition of high-performance athletes in France since the Mazeaud Law of 1975     

Sébastien Fleuriel  Manuel Schotté 《国际体育史杂志》2013,30(18):2707-2725

High-level athletes are accorded deferential treatment by the French state. They enjoy the status of ‘high-performance athletes', which entitles them to assistance from the state and a set of special provisions. This paper provides an overview of the political circumstances in which this status was originated and developed, from its initial recognition under the 1975 Sports Law to the paradoxical social effects that this state policy has had on its beneficiaries: the athletes themselves. The first part of the paper is based largely on a search of parliamentary archives relating to the adoption of this law, known as the Mazeaud law, while the second part is based on a survey and interviews conducted with a number of French athletes who were selected for the 1972 and 1992 Olympic Games in Munich and Barcelona respectively. In addressing these two key phases in the recognition of athlete status, its political development and its subsequent reception, the article illustrates the changes in both status and condition of high-performance athlete, based on very different time scales and inspired by fairly different expectations. Given that the present system aims to improve the position of athletes in terms of their post-competitive lives, the long-term benefit of representing France under these circumstances remains questionable.  相似文献   

Variable training: effects on velocity and accuracy in the tennis serve     

Hector Hernández-Davo  Tomás Urbán  Jose Manuel Sarabia  Casto Juan-Recio  Francisco Javier Moreno 《Journal of sports sciences》2013,31(14):1383-1388

Abstract

Variable practice has been shown to be an effective strategy to improve open motor skills. However, the usefulness of this procedure in closed motor skills remains controversial. The following study has the objective of analysing the effects of variability practice in the improvement of a closed skill. The skill studied has been the tennis serve. Thirty young tennis players (13 ± 1.52 years), divided in two groups, took part in this study. One group practiced in variable conditions and the other group in consistency conditions. Both groups performed 12 training sessions (60 serves/session). The variable practice group improved their accuracy significantly compared with the consistency group (F_3.25 = 3.078; P = 0.035). The velocity of serve increased after training in both groups (F_3.25 = 15.890; P = 0.001). The practice in variable conditions seems to be effective in improving the performance of the tennis serve.  相似文献   

Effects of mat characteristics on plantar pressure patterns and perceived mat properties during landing in gymnastics     

Pedro Pérez-Soriano  Salvador Llana-Belloch  Gaspar Morey-Klapsing  Jose Antonio Perez-Turpin  Juan Manuel Cortell-Tormo 《Sports biomechanics / International Society of Biomechanics in Sports》2013,12(4):245-257

Shock absorption and stability during landings is provided by both, gymnast ability and mat properties. The aims of this study were to determine the influence of different mat constructions on their energy absorption and stability capabilities, and to analyse how these properties affect gymnast's plantar pressures as well as subjective mat perception during landing. Six mats were tested using a standard mechanical drop test. In addition, plantar pressures and subjective perception during landing were obtained from 15 expert gymnasts. The different mats influenced plantar pressures and gymnasts' subjective perception during landing of gymnasts. Significant correlations between plantar pressures at the medial metatarsal and lateral metatarsal zones of the gymnasts' feet with the different shock absorption characteristics of the mats were found. However, subjective perception tests were not able to discriminate mat functionality between the six mats as no significant correlations between the mechanical mat properties with the subjective perception of these properties were found. This study demonstrated that plantar pressures are a useful tool for discriminating different landing mats. Using similar approaches, ideally including kinematics as well, could help us in our understanding about the influences of different mats upon gymnast-mat interaction.  相似文献   

Graduates on the labor market: Formal and informal post-school training investments     

Manuel Salas-Velasco 《Higher Education》2007,54(2):227-246

The purpose of this article is to obtain an understanding of which factors determine whether an university graduate receives formal on-the-job training or not and the amount of informal training received. Using a cross-sectional survey of Spanish graduates, this paper confirms that the informal training graduates receive in their jobs is more intense among doctors and lawyers – professions that require a more extensive period of learning before reaching the required level of skills. Likewise, those graduates that hold jobs requiring greater know-how than they can actually provide (infra-educated) also receive more informal training. In the case of formal training, the likelihood of taking part in in-house training programs is greater among civil servants and the employees of large private firms. However, the belief that workers with higher ability (a positive signal of a worker’s trainability) should have higher chances of receiving employer-provided formal training is not confirmed in this study.  相似文献   

Comparative analysis of expectancies of efficacy in in-service and prospective teachers   总被引：1，自引：0，他引：1  

Manuel J. de la Torre Cruz  Pedro F. Casanova Arias 《Teaching and Teacher Education》2007

This study examines the beliefs about effective teaching in student teachers and inservice teachers. We constructed a measurement composed of an adaptation in Spanish of the Gibson and Dembo's (1984. Teacher efficacy: A construct validation. Journal of Educational Psychology, 76, 569–582). Teacher Efficacy Scale and of the items included in Emmer and Hickman's (1991. Teacher efficacy in classroom management and discipline. Educational and Psychological Measurement, 51, 755–765) “classroom management/discipline efficacy” dimension. A total of 339 participants took part in the study. The factor analysis carried out of the results obtained showed three principal factors: classroom management/discipline efficacy, personal teaching efficacy and general teaching efficacy. Analyses which compared efficacy expectancies showed significant differences in the management and discipline dimension in favour of the group of working teachers, whereas an opposite pattern emerged in the general teaching dimension. In addition, there were differences in the management/discipline dimension in terms of the number of years’ experience in the group of inservice teachers. The interpretation of the results is expressed in terms of the Bandura's perceived self-efficacy theory. We also indicate some implications that the analysis of these expectancies may have for the training and professional development of teachers, and we suggest lines for future research.  相似文献   

Awarding teaching excellence: ‘what is it supposed to achieve?’ Teacher perceptions of student-led awards     

Manuel Madriaga  Krystle Morley 《Teaching in Higher Education》2016,21(2):166-174

Although there is lack of agreement as to what constitutes teaching excellence, there remains a steady effort to make an intangible, ambiguous, multifaceted concept incarnate in the form of ‘student-led’ teaching awards schemes within higher education institutions. What teaching staff say about such schemes have largely been ignored. This article attempts to address this gap in knowledge by accounting for the extent that academic teaching staff at one higher education institution in the UK value and perceive their teaching awards scheme. At the same time, this article presents some challenges in implementing a student-led teaching awards scheme for higher education institutions.  相似文献   

(Re)Configuring masculinities in an ethno-centric Australian community school: complexity and contradictions     

Sally Godinho  Dimitri Garas 《Gender and education》2012,24(1):83-99

This article draws on a case study of 15 boys aged between 13 and 14 years who attend an urban ethno-centric community school located in Melbourne, Australia. The study investigated how the boys' constructions of masculinity were mediated by a strong connectedness to their Greek cultural traditions and ideals. Data generated from focus group discussions provided insights into the complex ways in which the boys individually and collectively constructed their understandings of what it means to be a man. For most boys, maleness constituted cultural traditions and behaviours that must be learnt: a code of conduct which a boy acquires from his elders that is talked into existence and transmitted through a hegemonic discourse. Yet the findings also revealed the agency boys can exercise and the fluidity of their configurations: contradictions and inconsistencies being an inherent part of the recursive process of their gendered identity formation.  相似文献   

The coherence of vocational education and training in Norway and Spain: national traditions and the reshaping of VET governance in hybrid VET systems     

Manuel Souto-Otero 《Compare》2012,42(1):91-111

Coherence of national education and training systems is increasingly tabled in European policy debates. Leaning on literature about the emergence and consolidation of national education systems, this article explores the rationale for VET reforms in Norway and Spain by scrutinising attempts to strengthen the coherence of their VET systems. Coherence has been sought through the unification of different strands of vocational education; initial, continuing and active labour market policies (what we call ‘horizontal coherence’) and the mainstreaming of VET curricular elements; plus the systematisation of VET practices across educational levels (‘vertical coherence’). While both countries looked for coherence, their motivations, how they operationalised the term and the emphasis of their actions differed substantially. Spain has experienced a move from the three largely unrelated strands into a more unified system; Norway from a fragile VET system to the availability of more VET courses and apprenticeship arrangements at all educational levels.  相似文献