What is the future of electrical impedance spectroscopy in flow cytometry?     

Furkan Gke  Paolo S. Ravaynia  Mario M. Modena  Andreas Hierlemann 《Biomicrofluidics》2021,15(6)

More than 20 years ago, electrical impedance spectroscopy (EIS) was proposed as a potential characterization method for flow cytometry. As the setup is comparably simple and the method is label-free, EIS has attracted considerable interest from the research community as a potential alternative to standard optical methods, such as fluorescence-activated cell sorting (FACS). However, until today, FACS remains by and large the laboratory standard with highly developed capabilities and broad use in research and clinical settings. Nevertheless, can EIS still provide a complement or alternative to FACS in specific applications? In this Perspective, we will give an overview of the current state of the art of EIS in terms of technologies and capabilities. We will then describe recent advances in EIS-based flow cytometry, compare the performance to that of FACS methods, and discuss potential prospects of EIS in flow cytometry.  相似文献   

Study of silica nanoparticles – polysiloxane hydrophobic treatments for stone-based monument protection     

Lavinia de Ferri  Pier Paolo Lottici  Andrea Lorenzi  Angelo Montenero  Emma Salvioli-Mariani 《Journal of Cultural Heritage》2011,12(4):356

The growing interest in the conservation of historic buildings encourages the development of water-repellent materials and methodologies to consolidate and/or protect stones. Recently, particular attention was devoted to composites of inorganic oxides nanoparticles and hybrid siloxane or silicone polymers. Here we present a study on the water repellence of a thin protective coating obtained through sol-gel process starting from Glymo and Dynasylan 40®, and loading the silicate matrix with nano-sized silica particles (Aeroxide LE1® -Degussa-Evonik). The coatings were applied to limestone, sandstone and granite samples. The silica nanoparticles have been characterized by XRD, the siloxane matrix through micro-Raman spectroscopy, while the surface morphology was examined by SEM. The efficacy of the treatments has been evaluated through static contact angle measurements and capillary water absorption. The optical appearance of the coatings was evaluated by colorimetric measurements. Nanoparticles at suitable concentration gave high values for the static contact angle (up to ∼ 150°), for all stone species even for four months exposure to atmospheric conditions. The coatings, in the water capillary absorption tests, however, behave as expected only for granite even if the long-term water direct contact reduces the hydrophobicity.  相似文献   

A structural equation model predicting adults’ online learning self-efficacy     

Calaguas  Noriel P.  Consunji  Paolo Maria P. 《Education and Information Technologies》2022,27(5):6233-6249

Education and Information Technologies - We aimed to model the direct effects of the theorized relationships of academic self-efficacy, computer use self-efficacy, learning management system...  相似文献   

Development of a Three-Dimensional Phylogenetic Tree of the Plant Kingdom     

James E. Mickle  Paolo de Luca  Maria Rosaria Barone Lumaga  Manuela de Matteis Tortora 《Curator: The Museum Journal》1994,37(4):275-279

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