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11.
It's a Mystery     
In this column, a perinatal educator takes a humorous look at miscommunication.  相似文献   
12.
In this column, a childbirth educator explores the humorous side of miscommunication.  相似文献   
13.
In this column, the author takes a humorous look at conference attendees' name tags.  相似文献   
14.
Prenatal educator humorously reflects on misjudging class participants' personalities.  相似文献   
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16.
Lymphocyte subset estimations by flow cytometry in population-based studies require transportation of samples from the field site to the laboratory. As samples arrive late in the day they have to wait overnight before being processed. The effect of two possible approaches, sample storage for 24 h before staining and immediate staining with analysis after 24 h and 48 h were evaluated. Two sets of experiments were performed with EDTA (ethylenediamine tetra-acetate) anticoagulated peripheral blood. In the first experiment, after collection, each sample was divided into two portions. One portion was stained at the time of blood collection and the other 24 h later after keeping it at room temperature (38–45°C). In the second experiment, blood samples were stained within 1–2 h. Each sample was analyzed immediately upon completion of staining process and subsequently after 24 h and 48 h of storage at 4°C. Results suggest that blood collected in EDTA can be processed using whole blood lysis method, after storage at room temperature (38–45°C) for 24 h with some but not significant alteration in T-cell subsets. Storage at 4°C after staining for 24 h results in a lesser and insignificant loss of cells or alteration of T-cell subsets and may be the method of choice.  相似文献   
17.
The development of monoclonal antibodies combined with flow cytometry has revolutionized the analysis of lymphocyte subsets. These newer methods using the Q-prep leucocyte preparation system require only 1–2 ml of blood as compared to 10 ml required traditionally. One of the main impediments in the use of this superior technology in Indian laboratories has been the high cost of reagents. This study evaluated methods to reduce the cost of assays. In the first experiment from 26 healthy subjects, 2ml venous blood samples in EDTA (ethylenediamine tetra-acetate) were obtained. Each sample was divided into two equal portions, one portion was stained using diluted monoclonal antibody, whereas the other portion was stained using standard concentrations of antibodies. In the second experiment, blood samples from 12 subjects were again divided into 2 portions; one portion of each pair was processed using commercial Q-prep reagents while the other portion was processed using our own reagents. In the first experiment, which evaluated use of a diluted antibody against the standard recommended concentrations, a 5-tube panel that estimated CD3, CD4, CD8, CD20 was used. In the second experiment CD3, CD4 and CD8 were estimated. The total cost per sample for a 5-panel estimation was however reduced from $39.11 to $1.10. Given the proven advantages of using a whole blood stain-lyse method for T cell subset estimations, its use should be encouraged in developing country settings. With the suggested methods the whole blood Q-prep could be performed at appreciably reduced costs, without loss in precision.  相似文献   
18.
In this dialogue with Monica Ridgeway and Randy Yerrick’s Whose banner are we waving?: exploring STEM partnerships for marginalized urban youth, I engage the critical race theory (CRT) tenet of interest convergence. I first expand Derrick Bell’s (1980) initial statement of interest convergence with subsequent scholarly work in this area. I then explore ways CRT in general and interest convergence specifically have been applied in the field of education. Using this framing, I examine how interest convergence may be shed new insights into Monica Ridgeway and Randy Yerrick’s study. For example, the tenet of interest convergence is used to frame why it was beneficial for the White artist, Jacob, and the Achievement Scholars to collaborate in the service-learning mural. Then the idea of interest divergence is brought into explore the ways in which Jacob benefitted from his participation in the service learning project while the Achievement Scholars were left with an unfinished project which they had to problem solve. To conclude, I provide future directions for the application of interest convergence and divergence to issues facing science education.  相似文献   
19.
A qualitative nonexperimental thematic analysis was conducted at a hospital-based midwifery practice to explore the views of participants in group prenatal care and its impact on pregnancy, birth, and postnatal care. Ten women and three support people, recruited through purposive sampling, shared their feedback on the program. The findings conveyed three broad themes: program experience, midwife relationship, and support. Women enjoyed the opportunity for in-depth learning, and peer-group support led to normalizing of pregnancy concerns. Having support people as participants also helped during pregnancy, birth, and child care. The findings showed the enhanced opportunity for education, learning, and interpersonal support provided by CenteringPregnancy to expectant mothers had a positive impact on their pregnancy experiences.  相似文献   
20.
This paper characterizes oscillations found in block pulse function (BPF) domain identification of open loop first-order systems with step input. A useful condition for occurrence of such oscillations is presented mathematically. For any positive value of ‘ah’, oscillations are observed to occur, where h is the width of BPF domain sub-interval and 1/a is the time constant of the first-order system under consideration.  相似文献   
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