Evaluation of polymerase chain reaction for rapid diagnosis of clinically suspected tuberculous pleurisy     

Dil-Afroze  Dinesh Sharma  G. N. Dhobi  Sonaullah Shah  Rafiqa Eachkoti  Ishraq Hussain  Zafar A. Shah  Mushtaq A. Siddiqi 《Indian journal of clinical biochemistry : IJCB》2006,21(2):76-79

Pleural effusion is one of the commonest presentations of tuberculosis, the clinical manifestations being typically abrupt resembling bacterial pneumonia. Since delayed hypersensitivity is the underlying immune response, bacterial load is very low. Owing to these facts, tuberculous pleurisy as an extra-pulmonary disease poses a diagnostic dilemma. The conventional bacteriological methods rarely detect Mycobacterium tuberculosis in pleural fluid and are of limited use in diagnosis of tuberculous pleurisy. We evaluated the efficacy of polymerase chain reaction (PCR) in the diagnosis of tuberculous pleurisy by targeting the gene segment coding for MPB64 protein specific forMycobacterium tuberculosis. Based on the clinical criteria, 82 patients with lymphocytic exudative pleural effusion were included in the study. Patients were analyzed in two groups; one group consisting of 48 patients of tubercular pleural effusion confimed by various diagnostic procedures and another group of 34 patients comprising of non-tubercular pleural effusion. There were no false positive results by PCR and the specificity worked out to be 100%. Twenty two patients tested positive for Mantoux with a sensitivity of 45%. ZN-staining for AFB was found in samples from 15 patients (20% sensitivity). ADA was positive for 28 patients with a sensitivity of 53%. PCR was positive for 32/48 patients (67% sensitivity). Thus, PCR was found to be more sensitive than any other conventional method in diagnosis of clinically suspected tubercular pleurisy.  相似文献   

Oxidant-antioxidant status in colorectal cancer patients—Before and after treatment     

Sharmila Upadhya  Subramanya Upadhya  S. Krishna Mohan  K. Vanajakshamma  Mamatha Kunder  Seema Mathias 《Indian journal of clinical biochemistry : IJCB》2004,19(2):80-83

Erythrocyte antioxidant glutathione and malondialdehyde levels in erythrocytes and plasma glutathione S-transferase levels were estimated in patients with colorectal cancer and compared to controls. Further, the patients underwent four weeks of radiotherapy with adjuvant chemotherapy. The same parameters were estimated after four weeks of radiotherapy and compared with pretreatment levels. It was observed that there was a decrease in erythrocyte glutathione and malondialdehyde levels in patients with colorectal cancer compared to controls, but not in case of GST. However, after chemoradiotherapy, there were no statistically significant differences in all the parameters studied.  相似文献   

Effect of preoperative stress on serum lipoperoxide levels     

A. S. Sane  Shobha A. Chokshi  D. P. Barad  V. C. Shah  V. Mathur  S. C. Kukreti 《Indian journal of clinical biochemistry : IJCB》1989,4(1):26-30

To test the concept of intense psychogenic stress manifesting as organic lesion, effect of pre-operative stress on serum lipid peroxide levels in 30 otherwise healthy patients to undergo major abdominal elective surgery has been studied and compared with healthy controls. Significant elevations in serum lipid peroxide levels both on hospitalization and at pre-operative stage were noticed in comparison with healthy controls. However, serum lipid peroxide levels at pre-operative stage did not show significant fluctuations in comparison with those at the time of admission, suggestive of no probable further increment in autoxidation of unsaturated fatty acids of membrane lipids and proper handling by scavenging enzyme system.  相似文献   

Closing the loop on student feedback: the case of Australian and Scottish universities     

Mahsood Shah  Ming Cheng  Robert Fitzgerald 《Higher Education》2017,74(1):115-129

Universities have a long history of collecting student feedback using surveys and other mechanisms. The last decade has witnessed a significant shift in how student feedback is systematically collected, analysed, reported, and used by governments and institutions. This shift is due to a number of factors, including changes in government policy related to quality assurance, and the increased use of the results by various stakeholders such as governments, institutions, and potential students and employers. The collection, analysis, and reporting of results are systematically carried out in many institutions worldwide. However, how to use student feedback to effectively improve student learning experience remains an issue to be addressed. This paper will contribute to this debate by comparing how Australian and Scottish universities use student feedback results to inform improvements. Based on thematic analysis of external quality audit reports of all Australian and Scottish universities, this paper suggests that universities have systematic processes to collect student feedback using a range of mechanisms, but limited work is done to use the data to inform improvements. This paper argues the need for universities to genuinely listen to student voice by facilitating partnership between students and institutions to act on their feedback as part of quality assurance.  相似文献   

Role-based results redistribution for collaborative information retrieval     

Chirag Shah  Jeremy Pickens  Gene Golovchinsky 《Information processing & management》2010

We describe a new approach for algorithmic mediation of a collaborative search process. Unlike most approaches to collaborative IR, we are designing systems that mediate explicitly-defined synchronous collaboration among small groups of searchers with a shared information need. Such functionality is provided by first obtaining different rank-lists based on searchers’ queries, fusing these rank-lists, and then splitting the combined list to distribute documents among collaborators according to their roles. For the work reported here, we consider the case of two people collaborating on a search. We assign them roles of Gatherer and Surveyor: the Gatherer is tasked with exploring highly promising information on a given topic, and the Surveyor is tasked with digging further to explore more diverse information. We demonstrate how our technique provides the Gatherer with high-precision results, and the Surveyor with information that is high in entropy.  相似文献   

Fields medallists     

Nimish A. Shah 《Resonance》1999,4(11):80-83

  相似文献   

Asia in the edges: a narrative account of the Inter-Asia Cultural Studies Summer School in Bangalore     

Nishant Shah 《Inter-Asia Cultural Studies》2013,14(2):306-314

Abstract

The Inter-Asia Cultural Studies Summer School is a Biennial event that invites Masters and PhD students from around Asia to participate in conversations around developing and building an Inter-Asia Cultural Studies thought process. Hosted by the Inter-Asia Cultural Studies Society along with the Consortium of universities and research centres that constitute it, the Summer School is committed to bringing together a wide discourse that spans geography, disciplines, political affiliations and cultural practices for and from researchers who are interested in developing Inter-Asia as a mode of developing local, contextual and relevant knowledge practices. This is the narrative account of the experiments and ideas that shaped the second Summer School, “The Asian Edge” which was hosted in Bangalore, India, in 2012.  相似文献   

EGFR Mutation Analysis in Non-small Cell Lung Carcinoma Patients: A Liquid Biopsy Approach     

Jigna Joshi  Apexa Raval  Urja Desai  Vinal Upadhyay  Mansi Bhavsar  Kanisha Shah  Rakesh Rawal  Harsha Panchal  Franky Shah 《Indian journal of clinical biochemistry : IJCB》2021,36(1):51

In the era of the targeted therapy identification of EGFR mutation detection in lung cancer is extremely helpful to predict the treatment efficacy of EGFR tyrosine kinase inhibitors (TKIs). Unfortunately, the inadequacy and quality of the biopsy samples are the major obstacles in molecular testing of EGFR mutation in lung cancer. To address this issue, the present study intended to use liquid biopsy as the non-invasive method for EGFR mutation detection. A total of 31 patients with an advanced stage of lung cancer were enrolled in the study from which cell-free DNA (cfDNA) and FFPE tissue DNA was extracted. Extracted DNA samples were analyzed for further EGFR exon specific mutation analysis by ARMS-PCR. Data were analyzed statistically using SPSS software. In cfDNA samples, the prevalence of wild type EGFR was 48% while the prevalence of TKI resistant and TKI sensitive mutations were 3%. Conversely, in tissue DNA samples, the prevalence of wild type, TKI sensitive and TKI resistant mutations were 48%, 19%, and 3%, respectively. The overall concordance of EGFR mutation between cfDNA and tissue DNA was 83%. McNemar’s test revealed that there was no significant difference between EGFR expression of cfDNA and tissue DNA samples. Additionally, the significant-high incidence of TKI resistant mutations was observed in tobacco habituates, indicating the role of carcinogens present in the tobacco in developing resistant mutations. In conclusion, our data suggest that evaluation of EGFR mutation from cfDNA samples is practicable as a non-invasive tool in patients with advanced-stage of lung cancer.  相似文献   

Cognitive and metacognitive learning strategies among Arabic language students     

Ghazali Yusri  Nik Mohd Rahimi  Parilah M. Shah  Wan Haslina Wah 《Interactive Learning Environments》2013,21(3):290-300

This study investigates cognitive and metacognitive strategies in learning oral Arabic among students at Universiti Teknologi MARA (UiTM), Malaysia. The concept of these strategies was derived from the self-regulated learning framework, which consists of five components, namely rehearsal, elaboration, organization, critical thinking, and metacognitive strategies. The purposes of this study are to investigate the level of cognitive and metacognitive strategies used (1) among UiTM students; (2) between students with different prior experiences, namely, some of them had an experience of 5 years in learning Arabic in secondary school (abbreviated by SWE) and some of them did not have any experience at all (abbreviated by SNE); (3) between students of different gender; and (4) between students with the interaction of different gender and prior experience. The sample of this study consists of 183 students and employs a questionnaire adapted from the Motivated Strategies for Learning Questionnaire (MSLQ). The study revealed that (1) all UiTM students used cognitive and metacognitive strategies at a moderate level; (2) SWE scored significantly higher than SNE in all five components of cognitive and metacognitive strategies; (3) females scored significantly higher than males in rehearsal, organization, and metacognitive strategies; and (4) there were no statistically significant differences noted in all components between students with the interaction of prior experience and gender. This study had some classroom implications. It suggested that some improvement and changes in learning oral Arabic should be made in terms of selecting learning materials, implementing oral Arabic activities, and learning tasks, which will stimulate the use of all strategies, as well as conducting proficiency tests instead of achievement tests. Students should also be exposed to the learning techniques which used all these strategies extensively and collaborative activities may be carried out among students with mixed prior experience and gender.  相似文献   

Monitoring Trends in Student Satisfaction     

Grebennikov  Leonid  Shah  Mahsood 《Tertiary Education and Management》2013,19(4):301-322

Over the last decade, the assessment of student experience has gained significant prominence in Australian higher education. Universities conduct internal surveys in order to identify which of their services students rate higher or lower on importance and performance. Thus, institutions can promote highly performing areas and work on those needing improvement, while students are given an opportunity to influence decision-making in both academic and non-academic spheres of campus life. National student surveys conducted by the government aim to maintain quality assurance in the sector, benchmark outcomes, and, in some cases, reward better performing institutions. This paper outlines the findings of a study which monitored the entire student experience in a large metropolitan multi-campus university during 2005–2011. A specially designed biennial survey was repeated across the years, and completed over the period by 10,562 students from all key cohorts. This paper discusses the trends in perceived importance and performance of various university services and key issues the university has been addressing in order to enhance student experience. It concludes that: (a) the time series data provide a powerful lens into the university’s strategies, initiatives and actions which worked well and those needing further effort or adjustment; (b) it is the total experience of the university that shapes students’ judgements, not just what happens in the classroom; and (c) taking a systematic approach to student feedback, and acting on the results in a timely manner, can significantly improve student engagement and satisfaction.  相似文献