排序方式： 共有93条查询结果，搜索用时 0 毫秒
91.
92.
In this article, we present a microfluidic platform for passive fluid pumping for pump-free perfusion cell culture, cell-based assay, and chemical applications. By adapting the passive membrane-controlled pumping principle from the previously developed perfusion microplate, which utilizes a combination of hydrostatic pressure generated by different liquid levels in the wells and fluid wicking through narrow strips of a porous membrane connecting the wells to generate fluid flow, a series of pump-free membrane-controlled perfusion microfluidic devices was developed and their use for pump-free perfusion cell culture and cell-based assays was demonstrated. Each pump-free membrane-controlled perfusion microfluidic device comprises at least three basic components: an open well for generating fluid flow, a micron-sized deep chamber/channel for cell culture or for fluid connection, and a wettable porous membrane for controlling the fluid flow. Each component is fluidically connected either by the porous membrane or by the micron-sized deep chamber/channel. By adapting and incorporating the passive membrane-controlled pumping principle into microfluidic devices, all the benefits of microfluidic technologies, such as small sample volumes, fast and efficient fluid exchanges, and fluid properties at the micro-scale, can be fully taken advantage of with this pump-free membrane-controlled perfusion microfluidic platform. 相似文献
93.
The purpose of this study was to determine the optimum release conditions for the free throw in men's basketball. The study used hundreds of thousands of three-dimensional simulations of basketball trajectories. Five release variables were studied: release height, release speed, launch angle, side angle, and back spin. The free throw shooter was assumed to shoot at 70% and to release the ball 2.134 m (7 ft) above the ground. We found that the shooter should place up to 3 Hz of back spin on the ball, should aim the ball towards the back of the ring, and should launch the ball at 52 degrees to the horizontal. We also found that it is desirable to release the ball as high above the ground as possible, as long as this does not adversely affect the player's launch consistency. 相似文献