Assessment of Oxidative Stress and Inflammatory Process in Patients of Multiple Myeloma     

Moushumi Lodh  Binita Goswami  Nikhil Gupta  Surajeet K. Patra  Alpana Saxena 《Indian journal of clinical biochemistry : IJCB》2012,27(4):410-413

Multiple myeloma is a disseminated malignancy of monoclonal plasma cells that accounts for 15 % of all hematological cancers. The present study was conducted to evaluate the role of inflammation and oxidant-antioxidant dynamics in the etiology of this disease. The study population comprised of 20 cases of multiple myeloma and 20 healthy controls. The parameters evaluated were serum malondialdehyde (MDA), superoxide dismutase (SOD) and ferritin levels. The serum MDA levels were 1.9 ± 0.96 nmol/ml in cases as compared to 0.98 ± 0.55 nmol/ml in the controls. Similarly, a statistically significant difference was noted in the SOD and ferritin levels between the cases and controls (93.2 ± 23.8 vs. 210.1 ± 190.5 U/ml and 285.8 ± 216.4 vs. 131.8 ± 30.1 ng/ml respectively). Our study highlights the imbalance in the oxidant-anti oxidant mechanism and the role of smoldering inflammation in the etiology of multiple myeloma.  相似文献   

ApoStream?, a new dielectrophoretic device for antibody independent isolation and recovery of viable cancer cells from blood     

Vishal Gupta  Insiya Jafferji  Miguel Garza  Vladislava O. Melnikova  David K. Hasegawa  Ronald Pethig  Darren W. Davis 《Biomicrofluidics》2012,6(2)

Isolation and enumeration of circulating tumor cells (CTCs) are used to monitor metastatic disease progression and guide cancer therapy. However, currently available technologies are limited to cells expressing specific cell surface markers, such as epithelial cell adhesion molecule (EpCAM) or have limited specificity because they are based on cell size alone. We developed a device, ApoStream^™ that overcomes these limitations by exploiting differences in the biophysical characteristics between cancer cells and normal, healthy blood cells to capture CTCs using dielectrophoretic technology in a microfluidic flow chamber. Further, the system overcomes throughput limitations by operating in continuous mode for efficient isolation and enrichment of CTCs from blood. The performance of the device was optimized using a design of experiment approach for key operating parameters such as frequency, voltage and flow rates, and buffer formulations. Cell spiking studies were conducted using SKOV3 or MDA-MB-231 cell lines that have a high and low expression level of EpCAM, respectively, to demonstrate linearity and precision of recovery independent of EpCAM receptor levels. The average recovery of SKOV3 and MDA-MB-231 cancer cells spiked into approximately 12 × 10⁶ peripheral blood mononuclear cells obtained from 7.5 ml normal human donor blood was 75.4% ± 3.1% (n = 12) and 71.2% ± 1.6% (n = 6), respectively. The intra-day and inter-day precision coefficients of variation of the device were both less than 3%. Linear regression analysis yielded a correlation coefficient (R²) of more than 0.99 for a spiking range of 4–2600 cells. The viability of MDA-MB-231 cancer cells captured with ApoStream was greater than 97.1% and there was no difference in cell growth up to 7 days in culture compared to controls. The ApoStream device demonstrated high precision and linearity of recovery of viable cancer cells independent of their EpCAM expression level. Isolation and enrichment of viable cancer cells from ApoStream enables molecular characterization of CTCs from a wide range of cancer types.  相似文献   

The Dawkins Higher Education Plan: its Rationale and Implications     

Desh Gupta 《Higher Education Quarterly》1990,44(2):154-162

The Dawkins Plan is rapidly transforming the Australian higher education landscape. This paper explains its rationale. Like any controversial policy, there are winners and losers form this plan. These are brought out in the paper.  相似文献   

CERTAIN TECHNIQUES OF MULTIVARIATE ANALYSIS APPLIED TO DIFFERENT MEASURES OF INTER-ITEM RELATIONSHIPS FOR DEVELOPING UNIFACTOR TESTS1     

Ram K. Gupta 《Journal of Educational Measurement》1968,5(3):223-230

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Reflections on higher education in India in the light of the Robbins Report     

A. K. Das Gupta 《Minerva》1964,2(2):160-168

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Immunobiology of trophoblast cells     

Chandana Das  V. Senthil Kumar  Sayantani Basak  Surabhi Gupta  Sunesh Kumar 《Indian journal of clinical biochemistry : IJCB》2000,15(1):60-62

A female patient who had two primary cancers was investigated. Biochemical analysis of patient's serum for different parameters indicated an alteration in the metabolic process. Prognostically, the patient has shown favourable clinical features.  相似文献   

Changes in serum seromucoid following compensatory hyperparathyroidism: A sequel to chronic fluoride ingestion     

Sunil Kumar Gupta  R. C. Gupta  Kapil Gupta  H. P. Trivedi 《Indian journal of clinical biochemistry : IJCB》2008,23(2):176-180

This study was conducted to find out the possible underlying mechanism of various manifestation of fluorosis, a disease caused by excess ingestion of fluoride. For this the fluoride belt of Jaipur district was selected. The parameters selected were serum Parathyroid hormone, the levels of which are directly affected by fluoride intake. The levels of serum seromucoid, serum and leucocyte ascorbic acid, serum sialic acid (SSA) reflects ground substance metabolism. The study was conducted on two hundred children, selected from four areas (50 from each area) consuming water containing 2.4, 4.6, 5.6 and 13.6 mg/l of fluoride. Drinking water fluoride and serum fluoride were measured by Ion selective electrode method. Serum parathyroid by RIA and all other parameters were measured spectrophotometrically. The results revealed an increase in levels of fluoride, parathyroid hormone and seromucoid in serum with increasing water fluoride concentrations. Serum Calcium and serum ascorbic acid were found in normal range, how ever leucocyte ascorbic acid were decreased. A high positive correlation among fluoride concentration in drinking water and serum parathyroid hormone (r=0.967), and, serum parathyroid hormone and serum seromucoid concentration (r=0.935) was also observed The results indicated that secondary hyperparathyroidism due to hypocalcemic stress caused by excess fluoride ingestion disturbs normal metabolism of ground substance in calcified tissues of the body reflected as altered levels of the components of ground substance in the serum.  相似文献   

Electricity     

D. P. Sen Gupta 《Resonance》1997,2(9):38-47

The experiments of Michael Faraday and Joseph Henry in electromagnetic induction ushered in the era of electricity during the late nineteenth century. Human civilization has undergone a total change since then. This paper in two parts describes the basic principles of AC power generation, its transmission and distribution.  相似文献   

Conceptualization of Collection Care at the Napier Museum,Trivandrum, India     

Tanushree Gupta  Gabriela Krist  M. Velayudhan Nair  Johanna Wilk 《文物保护研究》2018,63(5):359-361

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Immuno-Modulatory Effect and Therapeutic Potential of <Emphasis Type="Italic">Brugia malayi</Emphasis> Cystatin in Experimentally Induced Arthritis     

Ravi Shankar Prasad Yadav  Vishal Khatri  Nitin Amdare  Kalyan Goswami  V. B. Shivkumar  Nitin Gangane  Maryada Venkata Rami Reddy 《Indian journal of clinical biochemistry : IJCB》2016,31(2):203-208

Helminths are known to modulate host’s immune system and understanding this modulation can help in identification of novel therapeutic agents for autoimmune diseases. In this study, we have assessed the immune-modulatory activity and the therapeutic effect of Brugia malayi recombinant cystatin (rBmCys) in methylated BSA (mBSA) induced arthritis using rodent model. Administration of rBmCys has suppressed the severity of mBSA-arthritis in mastomys by reducing paw swelling and other clinical disease parameters as evident from significantly decreased arthritic index. The anti-arthritic effect of rBmCys was also confirmed by decreased histopathological score for synovitis, bone erosion and fibrosis in the tissue sections of paws. Further, this therapeutic effect of cystatin was found to be associated with significantly decreased production of IFN-γ and TNF-α and increased release of IL-4 and IL-10 cytokines. These results implied that rBmCys treatment has alleviated mBSA-induced arthritis and thus can be a promising alternative agent for the treatment of arthritis.  相似文献