Detection of enzymes dehydrogenases and proteases inBrugia malayi filarial parasites     

Y. P. Bhandary  K. N. Krithika  Sandeep Kulkarni  M. V. R. Reddy  B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2006,21(1):1-7

Lymphatic filariasis caused mainly by infection fromW. bancrofti andB. malayi remains a major cause of clinical morbidity in tropical and subtropical countries. Analysis ofB. malayi mf, infective larval and adult worm lysates for the activity of enzymes led to the demonstration of activities of three key enzymes of carbohydrate metabolism viz., Malate dehydrogenase (MDH), Malic enzyme (ME) and Glucose-6-phosphate dehydrogenase (G6PDH) in all the three stages of the parasite. The specific activity of all the three dehydrogenases was significantly high in mf lysate compared to their activity in lysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel to their activity inlysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel electrophoresis (PAGE) using 7.5% non-gradient gel showed the presence of two isoforms of each of the three enzymes (MDH, ME & G6PDH) in mf lysate, while only one form of each enzyme was present in L₃ larval and adult worm lysates. Further proteolytic enzyme activity was demonstrated both in microfilarial and infective larval lysates ofB. malayi. While both mf and L₃ larval lysates showed optimal protease activity at alkaline pH of 9.0, the mf lysate showed increased activity also at pH 3.0. The infective larval lysate was markedly inhibited by Tosylamide-L-Phenylalanine chloromethyl ketone (TPCK), a thiol protease inhibitor, while the protease activity in mf lysate was significantly inhibited by both TPCK and a serine protease inhibitor Phenyl Methyl Sulphonyl Flouride (PMSF). In sodium do-decyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), using gelatin copolymerized gel, the microfilarial lysate showed 3 protease molecules of 40 kDa, 180 kDa and 200 kDa and the L₃ larval lysate had 6 protease molecules of 18, 25, 37, 49, 70 and 200 kDa size.  相似文献   

收缩爆聚的另一可能模型     

Tsui  K.H 《外藉学者来所报告集》1989,4(1):82-85

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Diagnostic and prognostic value of the urinary excretion of beta-aminoisobutyric acid in patients with urinary bladder cancer     

R. L. Gupta  K. Ramachandran  Monika  J. S. Chowhan 《Indian journal of clinical biochemistry : IJCB》1996,11(1):59-61

Urinary excretion of beta-aminoisobutyric acid (BAIB), was determined in 28 active and 12 inactive bladder cancer patients. Mean excretion of BAIB was higher in active cancer patients-187.8 mg/24 hours whereas it was 120.4 mg/24 hours in inactive cancer patients. The increased BAIB excretion in both disease groups was independent of tumour stage. BAIB appears to be a useful diagnositic aid in the assessment of bladder cancer patients.  相似文献   

Knowledge is more than information     

Müller-Merbach  Heiner 《知识管理研究与实践》2004,2(1):61-62

Knowledge Management Research & Practice -  相似文献   

Lipid peroxidation and glutathione peroxidase in ischemic heart disease     

K. S. Motghare  Anil Bhutey  B. B. Murrhar  Madhur Gupta  A. W. Meshram  Y. Balsubramanium 《Indian journal of clinical biochemistry : IJCB》2001,16(2):213-215

With the growing interest in the concept of free radicals in the pathogenesis of myocardial ischemia, it was thought worthwhile to study the changes in lipid peroxides and antioxidant enzyme glutathione peroxidase (GPx) in ischemic heart disease (IHD). The study was carried out on 76 patients of IHD-38 of acute myocardial infarction (AMI) and 38 of stable ischemic heart disease (SIDH). They were age and sex matched with 38 normal healthy controls. A significant increase (p<0.001) in lipid peroxides as malondialdehyde (MDA) (5.9±0.7 mmol/L) and a decrease in GPx (24.6±2.2 U/gmHb) was found in patients of AMI when compared with controls. There was no significant difference in these values in SIHD. Thus this study confirms the earlier findings that MDA and GPx are useful parameters in IHD and their magnitude is dependent on severity and/or duration of ischemia. We suggest that these tests would be of use in smaller institutions with limited facilities.  相似文献   

Dynamic thesaural systems: A bibliometric study of terminological and conceptual change in sociology and economics with application to the design of dynamic thesaural systems     

Lorna K. Rees-Potter 《Information processing & management》1989,25(6)

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Serum Ischemia-Modified Albumin in Preterm Babies with Respiratory Distress Syndrome     

Hasan Kahveci  Cuneyt Tayman  Fuat Laoğlu  Hüseyin Tugrul Celik  Nazan Kavas  Ömer Kılıç  Salih Aydemir 《Indian journal of clinical biochemistry : IJCB》2016,31(1):38-42

Infants with respiratory distress syndrome (RDS) may suffer from severe hypoxia, asphyxia. In this study, we aimed to evaluate serum ischemia-modified albumin (IMA) level as a diagnostic marker for hypoxia in preterm infants with RDS. Thirty-seven premature newborns with RDS were allocated as the study group and 42 healthy preterm neonates were selected as the control group. IMA was measured as absorbance unit (ABSU) in human serum with colorimetric assay method which is based on reduction in albumin cobalt binding. IMA levels were significantly higher in neonates with RDS as compared to the control group (P < 0.001). Cut-off value of IMA (ABSU) was 0.72, the sensitivity level was 91.9 %, the specificity was 78.6 %, positive predictive value was 79.1 % and negative predictive value was 91.7 % at RDS. Area under curve values was 0.93 (P < 0.001; 95 % CI, 0.88–0.98) in the receiver operating characteristic curve. We concluded that elevated blood IMA levels might be accepted as a useful marker for hypoxia in newborn with RDS.  相似文献   

N-acetyl-β-D-glucosaminidase,alanine aminopeptidase and protein: Creatinine ratio as early indicators of diabetic microangiopathy     

K. Srikrishna  A. S. Kanagasabapathy  Lily John 《Indian journal of clinical biochemistry : IJCB》1994,9(1):5-8

The urinary excretion patterns of N-acetyl-β-D-glucosaminidase (NAG), alanine aminopeptidase (AAP) and protein/creatinine ratio (UP/UCR) were studied in 133 diabetic subjects under treatment, 7 patients with established diabetic nephropathy (DN) and 79 carefully selected (age-matched) healthy subjects. NAG, AAP and UP/UCR were highly elevated in DN, while in diabetics urinary NAG levels correlated well with the degree of long-term metabolic control indicated by glycosylated hemoglobin (GHB or Hba1). Both AAP and UP/UCR were found to be more sensitive than NAG, but less specific. Urinary NAG and AAP assays thus offer simple, sensitive and non-invasive techniques for prognostic indication of the onset of microangiopathic changes in long-term diabetic subjects.  相似文献   

Serum lipid profile in diabetics with and without retinopathy     

P. K. Sharma  Poornima Chandak 《Indian journal of clinical biochemistry : IJCB》1992,7(2):203-204

Diabetics showed significantly raised levels of total and LDL cholesterol, (257.0±68.88 mg% 173.5±67.7mg% respectively) compared to controls matched with age, sex and socioeconomic status. No significant difference was observed in these parameters in diabetics with and without retinopathy.  相似文献   

Effect of Oestrogen on Altering the Serum and Urinary Levels of Calcium,Phosphate and Magnesium in Hysterectomised Women Compared to Natural Menopausal South Indian Women: A Case Control Study     

Yeldose Sonu  S. S. Avinash  Sreekantha  K. Arun Kumar  M. Malathi  A. R. Shivashankara 《Indian journal of clinical biochemistry : IJCB》2016,31(3):326-331

Given the paucity of studies conducted to know the effect of suddenness and earlier onset of endocrinological changes associated with hysterectomy, on the serum and urinary levels of calcium, magnesium and phosphate the present study was conducted to compare the levels of calcium, magnesium and phosphate in serum and urine of hysterectomised and natural menopausal south Indian women. This is a cross-sectional observational study. The study included three groups of 30 healthy premenopausal, 30 early surgical menopausal and 30 natural post menopausal women. Women suffering from any endocrine disease were excluded. Analysis was performed in serum and urine sample. The levels of calcium, magnesium and phosphate in serum and calcium/creatinine, magnesium/creatinine and phosphate/creatinine ratio were estimated in urine by spectrophotometric method. Hysterectomised women (serum calcium: 8.7 ± 0.09 mg/dl; urine calcium/creatinine: 0.16 ± 0.02) have significantly low serum calcium (p < 0.001) and high urinary calcium/creatinine (p = 0.002) ratio and post menopausal women (serum magnesium: 2.1 ± 0.03; serum phosphate: 4.4 ± 0.16; urinary calcium/creatinine: 0.17 ± 0.02; urinary magnesium/creatinine: 0.09 ± 0.01) have significantly high serum magnesium (p = 0.016), serum phosphate (p = 0.043) and high urinary calcium/creatinine (p = 0.002), magnesium/creatinine ratio (p = 0.025) compared to healthy pre menopausal women. Post menopausal women (serum calcium: 9.1 ± 0.08) have significantly high serum calcium and phosphate compared to hysterectomised women (serum phosphate: 3.93 ± 0.11). Hysterectomised women have significantly low serum calcium, oestrogen and high urinary calcium/creatinine ratio compared to healthy premenopausal women and low serum calcium and low serum phosphate compared to natural postmenopausal women. Natural postmenopausal women had low serum oestrogen and high serum magnesium, serum phosphate, urinary calcium creatinine ratio and urinary magnesium creatinine ratio compared to healthy premenopausal women.  相似文献