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以pLF3为模板,PCR扩增得到葡聚糖酶的启动子,并将启动子基因重新连接到切除启动子基因及葡聚糖酶基因的pLF3载体上,构建pLF3-pro载体.采用PCR的方法扩增朱黄青霉总DNA中的α-1-6-葡聚糖酶基因,并插入到pLF3-pro载体上,构建pLF3-Pro-Dex质粒,以大肠杆菌DH5α为宿主菌,酶切验证及PCR验证均表明成功构建了pLF3-Pro-Dex质粒,且重组质粒中的α-1-6-葡聚糖酶基因与朱黄青霉中的α-1-6-葡聚糖酶基因有93%的同源性. 相似文献
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The aim of this study was to determine whether there is an effect of time of day on the adaptation to strength training at maximal effort. Fourteen participants took part in this experiment. Their peak anaerobic power (Wingate anaerobic test) and peak knee extension torque at six angular velocities (1.05, 2.10, 3.14, 4.19, 5.24 and 6.29 rad x s(-1)) were recorded in the morning (between 07:00 and 08:00 h) and in the evening (between 17:00 and 18:00 h) just before and 2 weeks after a 6 week course of regular training. Seven of them trained only in the morning and seven only in the evening. Multivariate analysis of variance revealed a significant group x pre-/post-training x time of day interaction effect for peak torque and peak anaerobic power. Before training, in both groups, peak torque and peak anaerobic power were significantly higher in the evening than in the morning. After training, there was no significant difference in peak torque and peak anaerobic power between the morning and the evening for the morning training group. In contrast, in the evening training group, peak torque and peak anaerobic power were higher in the evening than in the morning. As a result of training, both peak torque and peak anaerobic power increased from their initial values as expected. The morning training group improved their peak anaerobic power significantly in the morning and in the evening, the absolute increase being larger in the morning than in the evening. The evening training group did not improve their peak anaerobic power in the morning, whereas it improved significantly in the evening. Although peak torque was significantly improved by training in the morning and evening in both groups, the absolute increase was greater in the morning than in the evening in the morning training group, whereas the opposite was the case for the evening training group. These results suggest that training twice a week at a specific hour increases the peak torque and the peak anaerobic power specifically at this hour and demonstrates that there is a temporal specificity to strength training. 相似文献
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Mougin F Bourdin H Simon-Rigaud ML Nguyen NU Kantelip JP Davenne D 《Journal of sports sciences》2001,19(2):89-97
The aim of this study was to determine the hormonal responses, which are dependent on the sleep wake cycle, to strenuous physical exercise. Exercise was performed after different nocturnal regimens: (i) a baseline night preceded by a habituation night; (ii) two nights of partial sleep deprivation caused by a delayed bedtime or by an early awakening; and (iii) two nights of sleep after administration of either a hypnotic compound (10 mg zolpidem) or a placebo. Eight well-trained male endurance athletes with a maximal oxygen uptake of 63.5 +/- 3.8 ml x kg(-1) x min(-1) (mean value +/- s(x)) were selected on the basis of their sleeping habits and their physical training. Polygraphic recordings of EEG showed that both nights with partial sleep loss led to a decrease (P< 0.01) in stage 2 and rapid eye movement sleep. A delayed bedtime also led to a decrease (P < 0.05) in stage 1 sleep. Zolpidem had no effect on the different stages of sleep. During the afternoon after an experimental night, exercise was performed on a cycle ergometer. After a 10-min warm-up, the participants performed 30 min steady-state cycling at 75% VO(2-max) followed by a progressively increased workload until exhaustion. The recovery period lasted 30 min. Plasma growth hormone, prolactin, cortisol, catecholamine and lactate concentrations were measured at rest, during exercise and after recovery. The concentration of plasma growth hormone and catecholamine were not affected by partial sleep deprivation, whereas that of plasma prolactin was higher (P < 0.05) during the trial after an early awakening. Plasma cortisol was lower (P < 0.05) during recovery after both sleep deprivation conditions. Blood lactate was higher (P < 0.05) during submaximal exercise performed after both a delayed bedtime and an early awakening. Zolpidem-induced sleep did not affect the hormonal and metabolic responses to subsequent exercise. Our results demonstrate only minor alterations in the hormonal responses to exercise after partial sleep deprivation. 相似文献
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Paul Geoffrey Taylor Kwee-Yum Lee Raul Landeo Damien Michael O'Meara Emma Millett 《Journal of sports sciences》2015,33(3):300-308
When characterising typical human movement profiles, the optimal number of trials analysed for each participant should ensure a stable mean. Sequential analysis is one method able to establish the number of trials to stability by assessing a moving point mean against a set bandwidth. As the total trial number determining this bandwidth is selected arbitrarily, the effect of applying different total trial numbers on the results of sequential analysis was investigated. Twenty participants performed 30 trials of overarm throwing, and sequential analyses were applied to three dimensional (3-D) kinematic data over 10, 20 and 30 trial numbers. We found a total of 20 to be the preferred trial number for sequential analyses. Erroneous results were produced consistently by 10 trial number groups, while moving point means were statistically unchanged after the 10th trial. Subsequently, sequential analyses were applied to 20 trials to establish trials to stability in discrete and time series elements of the 3-D kinematic data. The results suggest that a trial size between 13 and 17 provides stable means for overarm throwing kinematics. 相似文献
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Damien Page 《School Leadership & Management》2014,34(3):269-283
This article presents findings from a study of five head teachers who were responsible for the management of serious teacher misbehaviour (TMB) in England. In cases that included the downloading of extreme pornography on a school laptop and a sexual relationship with a pupil, the multiple impacts of TMB were potentially devastating to the well-being of pupils, staff and the reputation of the schools. The article highlights a number of strategies for managing serious TMB considered best practice by the head teachers as well as discussing other, more nuanced issues that accompany such cases including trust relations, the influence of gender and the difficulty of simultaneous immersion and detachment required of head teachers to effectively manage such cases. 相似文献
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Repeated high-intensity exercise in professional rugby union 总被引：1，自引：1，他引：0
The aim of the present study was to describe the frequency, duration, and nature of repeated high-intensity exercise in Super 14 rugby union. Time-motion analysis was used during seven competition matches over the 2008 and 2009 Super 14 seasons; five players from each of four positional groups (front row forwards, back row forwards, inside backs, and outside backs) were assessed (20 players in total). A repeated high-intensity exercise bout was considered to involve three or more sprints, and/or tackles and/or scrum/ruck/maul activities within 21 s during the same passage of play. The range of repeated high-intensity exercise bouts for each group in a match was as follows: 11-18 for front row forwards, 11-21 for back row forwards, 13-18 for inside backs, and 2-11 for outside backs. The durations of the most intense repeated high-intensity exercise bouts for each position ranged from 53 s to 165 s and the minimum recovery periods between repeated high-intensity exercise bouts ranged from 25 s for the back row forwards to 64 s for the front row forwards. The present results show that repeated high-intensity exercise bouts vary in duration and activities relative to position but all players in a game will average at least 10 changes in activity in the most demanding bouts and complete at least one tackle and two sprints. The most intense periods of activity are likely to last as long as 120 s and as little as 25 s recovery may separate consecutive repeated high-intensity exercise bouts. The present findings can be used by coaches to prepare their players for the most demanding passages of play likely to be experienced in elite rugby union. 相似文献
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Damien Page 《British Journal of Sociology of Education》2014,35(6):895-913
This article presents findings from a study of heads of construction schools within 14 further education colleges. Moving from a culture of hyper-masculinity, of tough, dirty work, construction lecturers and managers have to renegotiate their identity and work practices to become congruent with the inclusiveness of further education, leaving behind the aggression and discrimination embedded within the discourses and behaviours of the building site. However, they do bring with them desirable organisational masculinities, competitiveness and entrepreneurialism, that are highly valued by the contemporary college. The article presents findings of competitive and entrepreneurial practices in schools of construction and discusses the key role heads of construction play in bringing together multiple masculinities for the benefit of their school and their learners. 相似文献