Core-shell hydrogel beads with extracellular matrix for tumor spheroid formation     

L. Yu  S. M. Grist  S. S. Nasseri  E. Cheng  Y.-C. E. Hwang  C. Ni  K. C. Cheung 《Biomicrofluidics》2015,9(2)

Creating multicellular tumor spheroids is critical for characterizing anticancer treatments since they may provide a better model of the tumor than conventional monolayer culture. Moreover, tumor cell interaction with the extracellular matrix can determine cell organization and behavior. In this work, a microfluidic system was used to form cell-laden core-shell beads which incorporate elements of the extracellular matrix and support the formation of multicellular spheroids. The bead core (comprising a mixture of alginate, collagen, and reconstituted basement membrane, with gelation by temperature control) and shell (comprising alginate hydrogel, with gelation by ionic crosslinking) were simultaneously formed through flow focusing using a cooled flow path into the microfluidic chip. During droplet gelation, the alginate acts as a fast-gelling shell which aids in preventing droplet coalescence and in maintaining spherical droplet geometry during the slower gelation of the collagen and reconstituted basement membrane components as the beads warm up. After droplet gelation, the encapsulated MCF-7 cells proliferated to form uniform spheroids when the beads contained all three components: alginate, collagen, and reconstituted basement membrane. The dose-dependent response of the MCF-7 cell tumor spheroids to two anticancer drugs, docetaxel and tamoxifen, was compared to conventional monolayer culture.  相似文献   

Referee acknowledgment for 2014     

Hsueh-Chia Chang  Leslie Y. Yeo 《Biomicrofluidics》2015,9(1)

  相似文献   

题目：一种支持产品平台设计的多准则模块划分方法   总被引：2，自引：0，他引：2  

Wei WEI  Ang LIU  Stephen C.Y.LU  Thorsten WUEST 《浙江大学学报(A卷英文版)》2015,(1):1-10

目的：研究多准则约束下的产品模块划分方法,为企业建立稳健的模块化产品平台奠定基础。方法：采用改进的多目标进化算法对建立的多准则模块划分数学模型求解,并采用模糊集合评价机制进行最优解的寻取,得到基于多准则模块划分方法的产品模块划分结果。结论：通过改进的多目标进化算法求解多准则模块划分模型,能够得到有效支持产品平台设计的产品模块划分方案。通过与已有优化方法的比较验证了本文提出的多准则模块划分方法的优越性。  相似文献   

Relationship Reciprocation Modulates Resource Allocation in Adolescent Social Networks: Developmental Effects          下载免费PDF全文

Stephanie Burnett Heyes  Yeou‐Rong Jih  Per Block  Chii‐Fen Hiu  Emily A. Holmes  Jennifer Y. F. Lau 《Child development》2015,86(5):1489-1506

Adolescence is characterized as a period of social reorientation toward peer relationships, entailing the emergence of sophisticated social abilities. Two studies (Study 1: N = 42, ages 13–17; Study 2: N = 81, ages 13–16) investigated age group differences in the impact of relationship reciprocation within school‐based social networks on an experimental measure of cooperation behavior. Results suggest development between mid‐ and late adolescence in the extent to which reciprocation of social ties predicted resource allocation. With increasing age group, investment decisions increasingly reflected the degree to which peers reciprocated feelings of friendship. This result may reflect social‐cognitive development, which could facilitate the ability to navigate an increasingly complex social world in adolescence and promote positive and enduring relationships into adulthood.  相似文献   

论苏童笔下的“颂莲”式女性     

周茜  王艳芳 《乐山师范学院学报》2015,(7)

苏童是少数几个尤其擅长写女性的男性作家。他对女性的心理描写和细节把握都处理得恰到好处,非常自然。他笔下的女性一改过往的温柔善良、知书达理,相反的,大多都阴暗猥琐、粗俗野蛮、善于妒忌,非常具有特色,成为中国当代女性小说的独有景观。苏童的几个从女性视角描写的女主人公具有很多的共同特征,他将这些女性称为“颂莲”式女性。  相似文献   

Hands-On: Investigating the role of physical manipulatives in spatial training     

Katie A. Gilligan-Lee  Zachary C. K. Hawes  Ashley Y. Williams  Emily K. Farran  Kelly S. Mix 《Child development》2023,94(5):1205-1221

Studies show that spatial interventions lead to improvements in mathematics. However, outcomes vary based on whether physical manipulatives (embodied action) are used during training. This study compares the effects of embodied and non-embodied spatial interventions on spatial and mathematics outcomes. The study has a randomized, controlled, pre-post, follow-up, training design (N = 182; mean age 8 years; 49% female; 83.5% White). We show that both embodied and non-embodied spatial training approaches improve spatial skills compared to control. However, we conclude that embodied spatial training using physical manipulatives leads to larger, more consistent gains in mathematics and greater depth of spatial processing than non-embodied training. These findings highlight the potential of spatial activities, particularly those that use physical materials, for improving children's mathematics skills.  相似文献   

How do thinking styles and STEM attitudes have effects on computational thinking? A structural equation modeling analysis     

Haozhe Jiang  A. Y. M. Atiquil Islam  Xiaoqing Gu  Jia Guan 《科学教学研究杂志》2024,61(3):645-673

Computational thinking (CT) is vital for success in numerous domains. However, the nature, definition, and scope of CT are ill-defined, and research on how best to develop CT is very limited. This study focused on how thinking styles and STEM attitudes have effects on computational thinking. Using a proportionate stratified random sampling procedure, 1195 students from two universities were surveyed. A structural equation modeling analysis showed that students' thinking styles and STEM attitudes directly predicted their computational thinking skills and that thinking styles mediated the relationship between STEM attitudes and computational thinking skills. Thinking styles and STEM attitudes are strong predictors of CT skills. Based on the results, we recommended that the conceptualization of CT be broadened to reflect its trans-disciplinary nature within the context of STEM education. This study adds to the limited theoretical understanding of CT and CT-predictors in higher education, which has been studied much less than in K-12 education.  相似文献   

An evaluation framework for cross-lingual link discovery     

Ling-Xiang Tang  Shlomo Geva  Andrew Trotman  Yue Xu  Kelly Y. Itakura 《Information processing & management》2014

Cross-Lingual Link Discovery (CLLD) is a new problem in Information Retrieval. The aim is to automatically identify meaningful and relevant hypertext links between documents in different languages. This is particularly helpful in knowledge discovery if a multi-lingual knowledge base is sparse in one language or another, or the topical coverage in each language is different; such is the case with Wikipedia. Techniques for identifying new and topically relevant cross-lingual links are a current topic of interest at NTCIR where the CrossLink task has been running since the 2011 NTCIR-9. This paper presents the evaluation framework for benchmarking algorithms for cross-lingual link discovery evaluated in the context of NTCIR-9.  相似文献   

Effective cell collection method using collagenase and ultrasonic vibration     

Y. Kurashina  K. Takemura  S. Miyata  J. Komotori  T. Koyama 《Biomicrofluidics》2014,8(5)

This study proposes a novel cell collection method based on collagenase treatment and ultrasonic vibration. The method collects calf chondrocytes from a reusable metal cell culture substrate. To develop our concept, we calculated the natural vibration modes of the cell culture substrate by a finite element method, and conducted eigenvalue and piezoelectric-structural analyses. Selecting the first out-of-plane vibration mode of the substrate, which has a single nodal circle, we designed and fabricated the cell collection device. The excited vibration mode properly realized our intentions. We then evaluated the cell collection ratio and the growth response, and observed the morphology of the collected cells. The collagenase and ultrasonic vibration treatment collected comparable numbers of cells to conventional trypsin and pipetting treatment, but improved the proliferating cell statistics. Morphological observations revealed that the membranes of cells collected by the proposed method remain intact; consequently, the cells are larger and rougher than cells collected by the conventional method. Therefore, we present a promising cell collection method for adhesive cell culturing process.  相似文献   

认知心理学视域下体育教学方法分类模式的创新及重构*     

祝〓娅  黄依柱 《广州体育学院学报》2014,(5):46-49

通过文献资料、逻辑分析等方法对我国体育教学方法分类体系进行研究,旨在构建以认知心理学知识观为分类标准的体育教学方法分类模式。研究表明:现有体育教学方法分类模式不能为体育教学实践提供足够的支持,运用认知心理学关于知识表征类型的相关研究成果对体育教学目标进行解构,构建了言语图表信息类教学方法、智慧技能类教学方法、认知策略类教学方法和信念类教学方法四级分类模式。该分类模式对促进体育教学方法分类研究的科学化和体育教师合理选择与运用教学方法具有重要意义。  相似文献