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11.
Spheroids that are formed from aggregated cells have enhanced biological function compared to individual cells. In particular, hetero-spheroids composed of different types of cells, such as hepatocytes and endothelial cells, express tissue specific functions at a high level, which is advantageous for more precise drug screening and biological research. In this study, we propose rapid formation of size-controlled three-dimensional hetero-cell aggregates consisting of hepatocytes and endothelial cells using micro-rotation flow. Based on previous data, these aggregates are expected to ultimately become hetero-spheroids. The hepatocytes are coated with collagen gel films less than 200 nm thick, which were experimentally verified to increase adhesion strength between hepatocytes and endothelial cells. Gel-coated hepatocytes and endothelial cells are collected in an array by micro-rotational flow, thereby forming hetero-cell aggregates within 2 min. This array allowed the size of the three-dimensional cell aggregates to be hydrodynamically controlled, with standard deviations of less than 19%, by varying the cell density of the medium without altering the device geometry. Endothelial cells were successfully and uniformly dispersed in the aggregates. The proposed microfluidic device, with its capability of rapidly forming size-controlled hetero-cell aggregates, will offer an efficient experimental platform for future hetero-spheroid study that will contribute to drug screening and regenerative medicine. 相似文献
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Tomoyuki Matsuo Tsutomu Jinji Daisaku Hirayama Daiki Nasu Hiroki Ozaki Daisuke Kumagawa 《Sports biomechanics / International Society of Biomechanics in Sports》2018,17(2):180-191
The objectives of this study were to investigate middle finger movements and dynamics of ball movements around the instant of ball release during baseball pitching. Baseball pitching from an indoor mound among 14 semi-professional pitchers was captured using a motion capture system with 16 high-speed cameras (1,000 Hz). Kinematics of middle finger joints, ball rotation, and force applied to the ball were calculated. The proximal and distal interphalangeal joints continued to extend until the instant of ball release, then abruptly flexed. The abrupt flexion lasted for only several milliseconds, followed by a short extension phase. The finger made a quick double cycle of extension-flexion movement, suggesting that it attained high stiffness resulting from co-contraction. The ball began to roll up to the tip of the finger 8 ± 1 ms before ball release owing to the start of extension or the increased angular velocity of extension for the proximal interphalangeal joint. A mean force of 195 ± 27 N was applied in the proximal direction of the hand at the same time as the beginning of ball rolling, and a mean force of 109 ± 22 N was applied to the throwing direction just before ball release. 相似文献
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Kai Makita Mika Yamazaki Hiroki C. Tanabe Takahiko Koike Takanori Kochiyama Hirokazu Yokokawa Haruyo Yoshida Norihiro Sadato 《Mind, Brain, and Education》2013,7(4):213-224
Psychological research suggests that foreign‐language vocabulary acquisition recruits the phonological loop for verbal working memory. To depict the neural underpinnings and shed light on the process of foreign language learning, we conducted functional magnetic resonance imaging of Japanese participants without previous exposure to the Uzbek language using learning of novel Uzbek words. During encoding, spoken Uzbek words and corresponding visual objects were shown, and subjects either overtly repeated the words (phonological rehearsal) or overtly rehearsed numbers (phonological suppression). Phonological rehearsal improved the encoding performance. A learning‐related decrease in rehearsal‐specific activation was found in the left fusiform gyrus, right inferior temporal gyrus, and right cerebellum. Recollection of the phonologically rehearsed words activated the right cerebellum and left fusiform gyrus more prominently than recollection of the phonologically suppressed words in a performance‐dependent manner. The phonological loop might provide the temporal and fragile registration of the articulatory pattern that is converted into a more durable form in the right cerebellum, which is in turn integrated with the object information in the fusiform gyrus. 相似文献
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Tomoyuki Matsuo Tsutomu Jinji Daisaku Hirayama Daiki Nasu Hiroki Ozaki 《Sports biomechanics / International Society of Biomechanics in Sports》2016,15(2):220-233
This study was conducted to determine whether a supination phase of the forearm exists around ball release (BRL), and, if present, to determine whether this supination is explained by a reaction force or by the body configuration required for this task. A 16-camera motion analysis system with a sampling frequency of 1,000 Hz recorded 20 healthy male semi-professional pitchers pitching from an indoor pitching mound. A short supination phase around BRL was confirmed for all participants in the current study. Correlation analyses revealed that the supination angle at BRL had significant relationships with several measurements of shoulder movement kinematics. Mechanical work analysis of the forearm’s longitudinal axis revealed several variations in joint power curve and various patterns of mechanical work among the participants, suggesting that a reaction force originating from accelerating a ball might not be the main cause of supination. The raw data also were down-sampled to a sampling frequency of 250 Hz, to match previous studies and to investigate the discrepancy among previous studies concerning the existence of the supination phase. The experience of participants and methodological differences, such as the definition of BRL and the time-normalisation technique, influenced whether the supination phase was observed. 相似文献
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Aerodynamic properties of an archery arrow 总被引：1，自引：0，他引：1
Takeshi Miyazaki Keita Mukaiyama Yuta Komori Kyouhei Okawa Satoshi Taguchi Hiroki Sugiura 《Sports Engineering》2013,16(1):43-54
Two support-interference-free measurements of aerodynamic forces exerted on an archery arrow (A/C/E; Easton Technical Products) are described. The first measurement is conducted in a wind tunnel with JAXA’s 60 cm Magnetic Suspension and Balance System, in which an arrow is suspended and balanced by magnetic force against gravity. The maximum wind velocity is 45 m/s, which is less than a typical velocity of an arrow (about 60 m/s) shot by an archer. The boundary layer of the arrow remains laminar in the measured Re number range (4.0 × 103 < Re < 1.5 × 104), and the drag coefficient is about 1.5 for Re > 1.0 × 104. The second measurement is performed by a free flight experiment. Using two high-speed video cameras, we record the trajectory of an archery arrow and analyze its velocity decay rate, from which the drag coefficient is determined. In order to investigate Re number dependence of the drag coefficient in a wider range (9.0 × 103 < Re < 2.4 × 104), we have developed an arrow-shooting system using compressed air as a power source, which launches the A/C/E arrow at an arbitrary velocity up to 75 m/s. We attach two points (piles) of different type (streamlined and bullet) to the arrow-nose. The boundary layer is laminar for both points for Re less than about 1.2 × 104. It becomes turbulent for Re larger than 1.2 × 104 and the drag coefficient increases to about 2.6, when the bullet point is attached. In the same Re range, two values of drag coefficient are found for the streamlined point, of which the lower value is about 1.6 (laminar boundary layer) and the larger value is about 2.6 (turbulent boundary layer), confirming that the point-shape has a crucial influence on the laminar to turbulent transition of the boundary layer. 相似文献