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In this report, we demonstrate a simple and low cost method that can be reproducibly used for fabrication of microfluidic devices in nitrocellulose. The fluidic patterns are created via a laser-based direct-write technique that induces polymerisation of a photo-polymer previously impregnated in the nitrocellulose. The resulting structures form hydrophobic barriers that extend through the thickness of the nitrocellulose and define an interconnected hydrophilic fluidic-flow pattern. Our experimental results show that using this method it is possible to achieve microfluidic channels with lateral dimensions of ∼100 μm using hydrophobic barriers that form the channel walls with dimensions of ∼60 μm; both of these values are considerably smaller than those that can be achieved with other current techniques used in the fabrication of nitrocellulose-based fluidic devices. A simple grid patterned nitrocellulose device was then used for the detection of C-reactive protein via a sandwich enzyme-linked immunosorbent assay, which served as a useful proof-of-principle experiment. 相似文献
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Higher Education - In recent years, government policies that target the rapid internationalization of Japanese higher education have provided new career opportunities especially for scholars with... 相似文献
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Ioannis Papadopoulos 《Science & Education》2014,23(1):61-77
This paper describes a classroom experiment where students use techniques found in the history of mathematics to learn about an important mathematical idea. More precisely, sixth graders in a primary school follow Archimedes’s method of exhaustion in order to compute the number π. Working in a computer environment, students inscribe and circumscribe regular polygons inside and around a circle in order to find the approximate area of the circle. They then compute the ratio of that approximation to the area of a square with side-length equal to the radius of the circle. This ratio indicates how many times larger the area of the circle is than the area of the square. Mirroring Archimedes’s findings, students discover that as they increase the number of sides in their polygons, the numerical results they obtain convince them that this number is almost equal to 3.14. 相似文献
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This paper uses an autoethnography to recount my experiences with SportHelp, a UK youth sports charity. Using a layered account format, which jumps through time and space, I demonstrate the extent to which neoliberal values have influenced the continuity and change of SportHelp. This paper does not constitute an attack on the charity, its staff, nor the charity sector. The focus is on how the wider neoliberal context shapes how SportHelp operates. The findings are analysed in terms of Foucault’s (2008, The birth of biopolitics. Lectures at the Collége de France, 1978–79. Basingstoke: Palgrave Macmillan) notion of governmentality by examining SportHelp’s monitoring and reporting practices, as well as the managers’ use of New Public Management discourse. The conclusion reflects on the extent to which neoliberal governmentality, though in some instances beneficial for SportHelp, ultimately does more harm than good. This paper, by offering an ‘insider’s view’, adds to the literature calling for a change in how policy makers and funders shape the current hypercompetitive socio-political landscape. Charities should be supported, not discouraged, to develop holistic programmes that move beyond ‘economic rationales’ and are capable of addressing the multifaceted needs of their service users. 相似文献