Identification of drug-resistantMycobacterium tuberculosis by single strand conformation polymorphism and cleavase fragment length polymorphism     

Manju Sharma  Manuel Altamirano 《Indian journal of clinical biochemistry : IJCB》2000,15(2):88-93

Twenty isolates ofMycobacterium tuberculosis resistant to rifampicin(RIF), isoniazid(INH) and streptomycin(STR) were analysed by Polymerase Chain Reaction (PCR) amplification of rpoB, katG and rrs genes to evaluate comparative diagnostic significance of genetic assays. Mutations were identified by single strand conformation polymorphism (SSCP) and cleavase fragment length polymorphism (CFLP) and were confirmed by DNA sequencing. SSCP of 4 RIF resistant and 14 INH resistant isolates showed an extra peak at the level of 75-bp and 85-bp respectively, while 2 STR resistant isolates showed 2 peaks with 9 bases difference. CFLP showed a different pattern among RIF, INH and STR sensitive and resistant isolates Thus SSCP and CFLP can be used as alternative diagnostic methods for identification of mutations in RIF, INH and STR resistant strains of M.tuberculosis.  相似文献   

Breast and prostate cancer     

B. K. Sharma  A. Ray 《Indian journal of clinical biochemistry : IJCB》2000,15(1):110-117

There are interesting similarities between carcinomas of the breast and prostate. Both genetic and exogenous factors are probably important in the development of breast and prostate cancers. Since there is an alarming increasing trend in the incidence of both cancers worldwide including India, high level of apprehension/awareness has been created among the general educated population. Furthermore, both cancers are strongly linked with the expression of the c-erbB-2 oncogene which has been the focus of basic research in the recent past. This gene belongs to the family of growth factor receptors and it has important implication in diagnosis and future treatment modalities.  相似文献   

Role of Cultural Intelligence in Acculturation: Explorations on a Physiognomic Minority Diaspora in India     

Namrata Sharma 《Journal of Intercultural Communication Research》2019,48(3):274-291

The aim of this study was to analyse how cultural intelligence (CQ) influences the acculturation process and adaptive outcomes of a physiognomic minority indigenous cultural group in India. This study (N = 246) concerning the northeastern (NE) Indian diaspora in Indian cities outside the NE region. Path analysis showed that integration and assimilation predicted higher adaptation; while marginalization and separation lowered adaptation. Likewise, CQ positively predicted adaptation and also moderated the relationship between acculturation strategies and adaptation. Therefore, findings reflect the flexible facilitative role of CQ in promoting adaptation of minority groups in acculturation context.  相似文献   

Current Status and Future Directions for Cultural Intelligence     

Namrata Sharma 《Journal of Intercultural Communication Research》2017,46(1):96-110

The concept of cultural intelligence was formulated to ease and understand multicultural interactions in business organizations. Based on the theory of multiple intelligence; it is the cross-cultural facet of intelligence that assists adaptive cultural adjustment. Due to its implications for the contemporary world witnessing increased cultural contact and clashes; it has attracted multidisciplinary scholarly interest. This article reviews the development, validation and major approaches to the construct, along with a focus on its critical analysis. Significance of the construct in promoting intercultural discourse to encourage pacific multicultural existence is implicated. Furthermore, suggestions are made to extend the scope of cultural intelligence research and integrate it in diverse fields encompassing intercultural contact and communication.  相似文献   

The impact of early life trauma on health and disease: the hidden epidemic     

Dev Sharma 《Educational Psychology in Practice》2018,34(1):105-106

  相似文献   

Pre‐service teachers’ attitudes towards inclusion of students with high support needs in regular classrooms in Bangladesh          下载免费PDF全文

Tariq Ahsan  Umesh Sharma 《British Journal of Special Education》2018,45(1):81-97

In the process of educational reforms for promoting equity and inclusion in education, pre‐service teacher preparedness has been identified as a vital factor that has an impact on the success of inclusive education. Bangladesh, like other parts of the world, has taken various initiatives to promote inclusive education in the country context. This study is an attempt to explore factors related to pre‐service teachers’ preparedness for inclusive education in Bangladesh. The current study is an extension of the work completed in previous studies and specifically examined participants’ attitudes and factors that influenced their attitudes. Following a mixed method design, this multi‐phase study measured 1,623 pre‐service teachers’ attitudes towards inclusive education in phase 1. By applying a multiple regression procedure, several variables were identified that impacted on pre‐service teachers’ attitudes towards inclusion. The results indicated that Bangladeshi pre‐service teachers hold a less positive attitude towards the inclusion of children who have high support needs (that is, those who use Braille or sign language, or those who need to have an Individualised Academic Programme). In order to better understand the findings about apprehensive attitudes among pre‐service teachers, semi‐structured interviews were conducted with six institutional heads of pre‐service teacher education institutions. The interviews provided a new perspective on apprehensive attitudes among participants. Several curriculum reform suggestions are made that may have implications for policy‐makers and researchers in Bangladesh and beyond.  相似文献   

One Year of COVID-19: The “New Normal”     

Prasenjit Mitra  Sanjeev Misra  Praveen Sharma 《Indian journal of clinical biochemistry : IJCB》2021,36(1):1

  相似文献   

Epigenetics in Lead Toxicity: New Avenues for Future Research     

Prasenjit Mitra  Sanjeev Misra  Praveen Sharma 《Indian journal of clinical biochemistry : IJCB》2021,36(2):129

  相似文献   

A Three-Dimensional Print Model of the Pterygopalatine Fossa Significantly Enhances the Learning Experience     

Jordan A. Tanner  Beeran Jethwa  Jeff Jackson  Maria Bartanuszova  Thomas S. King  Arunabh Bhattacharya  Ramaswamy Sharma 《Anatomical sciences education》2020,13(5):568-580

The pterygopalatine fossa (PPF) is a bilateral space deep within the skull that serves as a major neurovascular junction. However, its small volume and poor accessibility make it a difficult space to comprehend using two-dimensional illustrations and cadaveric dissections. A three-dimensional (3D) printed model of the PPF was developed as a visual and kinesthetic learning tool for completely visualizing the fossa, its boundaries, its communicating channels, and its neurovascular structures. The model was evaluated by analyzing student performance on pre- and post-quizzes and a student satisfaction survey based on the five-point Likert scale. The first cohort comprised of 88 students who had never before studied the PPF. The second cohort consisted of 30 students who were previously taught the PPF. Each cohort was randomly divided into a control group who were provided with a half skull and an intervention group that were provided with the 3D printed model. The intervention group performed significantly better on the post-quiz as compared to the control group in cohort I (P = 0.001); while not significant, it also improved learning in cohort II students (P = 0.124). Satisfaction surveys indicated that the intervention group found the 3D printed model to be significantly more useful (P < 0.05) as compared to the half skull used by the control group. Importantly, the effect sizes for cohorts I and II (0.504 and 0.581, respectively) validated the statistical results. Together, this study highlights the importance of 3D printed models as teaching tools in anatomy education.  相似文献   

Utility of serum LDH isoforms in the assessment of mycobacterium tuberculosis induced pathology in TB patients of Sahariya tribe     

P. R. Sharma  S. Jain  R. N. K. Bamezai  P. K. Tiwari 《Indian journal of clinical biochemistry : IJCB》2010,25(1):57-63

The present study was carried out in the Sahariya tribe of Central India, which reportedly have high prevalence of pulmonary tuberculosis. Total serum LDH and its tissue specific isoforms were estimated in TB patients and matched healthy controls to test the utility of LDH as diagnostic marker for tuberculosis. About 210 sputum positive cases and 328 age and sex matched sputum negative controls were recruited. The spectrophotometeric and densitometric analysis of each LDH isoform was carried out in both cases and controls. The mean values of serum LDH were estimated and compared for each class by t-test. The statistical comparisons were made between sputum negative controls and sputum positive cases by Mann-Whitney’s U test. The spectrophotometric estimation of serum LDH revealed significant (P=0.0016) increase in its level in cases (290 IU/L) as compared to controls (248 IU/L). The densitometric analysis of individual LDH isoforms in cases and controls demonstrated significant elevation in LDH1 (P>0.05), LDH2 (P>0.05) and LDH3 (P<0.005) in sputum positive cases in comparison to sputum negative controls. Our study revealed a positive correlation between serum LDH level and the presence of mycobacteria and their load, suggesting utility of LDH as an important diagnostic marker of tuberculosis induced stress, at least in tribal areas lacking access to modern clinical tests.  相似文献