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91.
92.
Bio-electrosprays (BESs) provide a means of precisely manipulating cells and thus have the potential for many clinical uses such as the generation of artificial tissues∕organs. Previously we tested the biological safety of this technology with a variety of living cells and also embryos from the vertebrate model organisms Danio rerio (zebrafish) and Xenopus tropicalis (frog). However, the viability and fertility of the treated embryos could not be fully assessed due to animal licensing laws. Here we assay the viability and fertility of Drosophila melanogaster (fruit fly) embryos in conjunction with the bio-electrospray procedure. Bio-electrosprayed Drosophila embryos developed into fully fertile adult flies that were indistinguishable from wild-type. Thus, we demonstrate that the bio-electrospray procedure does not induce genetic or physical damage that significantly affects the development or fertility of a multicellular organism. This study along with our previous investigations demonstrates the potential of this approach to be developed for the precise manipulation of sensitive biological materials.  相似文献   
93.
The aim of this study was to examine the potential motivational and behavioural benefits of two peer tutoring programmes for tutors in a sport setting. Differences between the sexes were also explored. Thirty two college-age males and females, all novices on a French boxing task, were assigned to a 2 x 2 [sex x training type: physical practice associated with trained peer tutoring (TPT) vs physical practice associated with untrained peer tutoring (UPT)] factorial design. All participants were given six French boxing lessons of 2 h each. The TPT programme included structured methods to prepare the participants to fulfil their role of tutors, whereas the UPT programme did not. The results demonstrated that the TPT programme resulted in higher scores for coaching skills. Furthermore, interaction effects revealed that the TPT programme yielded better offensive outcomes for males and better defensive outcomes for females. Although the UPT participants expressed higher self-efficacy, no differences emerged for intrinsic motivation and causal attributions. Finally, male tutors displayed higher self-efficacy and offensive outcomes than female tutors. The results are discussed in the light of previous findings in the educational and sport psychology literature.  相似文献   
94.
During the 1960s and 1970s, “traditional” secondary educational systems in various Western European countries made way for comprehensive education curricula. In contrast to the reforms within the “intellectual” subjects, the field of physical education (PE) remained largely underexposed in research. This study focuses on PE reform in Belgium, the first country in the world (apart from Sweden itself) to introduce, in 1908, so-called “Swedish gymnastics” in all levels of state public education and, in 1968, the last country apart from Portugal to abandon it. The question arises as to who was responsible for Swedish gymnastics being applied for so long and, ultimately, how and by whom reform was implemented. The results show that the power of the past and the positions of “Swedists” in the Government Inspectorate, universities and professional associations, perpetuated the domination of Swedish gymnastics in Belgian education. The final “dynamisation” of the subject of PE around 1968 did not come about through “revolution” but a “natural” generational transition, whereby a new guard of young government inspectors with a drive for reform took over from their Swedish-minded predecessors. The educational climate prevailing in Belgium at the end of the 1960s facilitated this reform. The state university PE institutes lost their position as pedagogical profile keepers during this process of transformation, while the hidden curriculum of the (Catholic) university institute in Leuven implicitly shaped the educational climate. Further comparative international research into the (changed) profile, position and power of school inspectors in educational reform appears necessary.  相似文献   
95.
We report how cell rheology measurements can be performed by monitoring the deformation of a cell in a microfluidic constriction, provided that friction and fluid leaks effects between the cell and the walls of the microchannels are correctly taken into account. Indeed, the mismatch between the rounded shapes of cells and the angular cross-section of standard microfluidic channels hampers efficient obstruction of the channel by an incoming cell. Moreover, friction forces between a cell and channels walls have never been characterized. Both effects impede a quantitative determination of forces experienced by cells in a constriction. Our study is based on a new microfluidic device composed of two successive constrictions, combined with optical interference microscopy measurements to characterize the contact zone between the cell and the walls of the channel. A cell squeezed in a first constriction obstructs most of the channel cross-section, which strongly limits leaks around cells. The rheological properties of the cell are subsequently probed during its entry in a second narrower constriction. The pressure force is determined from the pressure drop across the device, the cell velocity, and the width of the gutters formed between the cell and the corners of the channel. The additional friction force, which has never been analyzed for moving and constrained cells before, is found to involve both hydrodynamic lubrication and surface forces. This friction results in the existence of a threshold for moving the cells and leads to a non-linear behavior at low velocity. The friction force can nevertheless be assessed in the linear regime. Finally, an apparent viscosity of single cells can be estimated from a numerical prediction of the viscous dissipation induced by a small step in the channel. A preliminary application of our method yields an apparent loss modulus on the order of 100 Pa s for leukocytes THP-1 cells, in agreement with the literature data.  相似文献   
96.
There is an increasing demand for spatial data within the social sciences as such data promise to contribute to a better understanding of how the concrete living environment influences individuals’ attitudes and behaviors. Spatial data can complement data from surveys; however, as of yet there is no spatial data infrastructure for the social sciences in Germany that facilitates merging of spatial data with survey data. This article explores avenues for creating such a spatial data infrastructure (SDI) for the social sciences in Germany. We examine the role that librarians, archivists, and curators can play in offering spatial data infrastructure services for social scientists, and show the scope and nature of necessary tasks in areas such as harmonization, archiving, dissemination, and user support. As the case of Germany is similar to that of other European countries, we expect our results to be helpful in the creation of SDIs in other countries as well. This article suggests technical and organizational measures for merging survey data and spatial data in compliance with German privacy legislation. Measures for storage and dissemination of enriched survey data, for example by providing restricted access to the data in a secured environment, also are explored.  相似文献   
97.
In a course on biological psychology and neuropsychology, study questions were provided that also appeared as test questions in the course exam. This method was introduced to support students in active processing and reproduction of the study texts, and study planning. Data were gathered to test the hypothesis that study question use would be beneficial to course grades. In addition, the way in which students evaluated the method was studied. It appeared that the number of study questions answered prior to the exam had a positive effect on course grades. Students were generally positive about the use of the questions in terms of their study planning.  相似文献   
98.
This article explores characteristics of school violence inFrance and the privileged means by which school counsellors can address this increasinglywidespread problem. In the first section, the status of counsellors in theFrench schools is outlined, and it is shown that counselling activities are reallyonly undertaken for students in junior high or high school. Two importantcharacteristics of the French view of school violence are: The experts in psychology takelittle interest in problems of school violence; and adults, rather than students,are seen as the primary victims. In this context, counsellors try to reduceschool violence primarily through training and reflecting with school staff. Severalempirical arguments suggest, in effect, that the adults' perceptions of the students is adetermining factor in the social regulation of school violence. This violence consistsmostly of incivilities that are only slightly reprehensible but oftenunbearable for certain teachers. Depending on whether the school staff try to understandthe incivilities or can only see them as pure savagery, they either try to controlthem through education or they lose confidence in their professional mission.The goal of counsellors is both to help the personnel develop theircapacities and motivate them to analyze the students' behavior, and to engender a senseof team spirit.  相似文献   
99.
European Journal of Psychology of Education - The massification and diversification of the student population who enters university has become a challenge for Higher Education institutions. A...  相似文献   
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