Molecular Characterization of Iranian Patients with Possible Familial Hypercholesterolemia     

Farrokhi E  Shayesteh F  Asadi Mobarakeh S  Roghani Dehkordi F  Ghatreh Samani K  Hashemzadeh Chaleshtori M 《Indian journal of clinical biochemistry : IJCB》2011,26(3):244-248

Familial hypercholesterolemia (FH) is an autosomal dominant disorder of lipoprotein metabolism caused mainly by mutations in the low-density lipoprotein receptor (LDLR) and apolipoprotein B 100 (APOB) genes. Until now, the molecular basis of FH has been demonstrated in detail in many populations, but there is still very limited Molecular data concerning FH in Iran. The aim of this study was to characterize the LDLR and APOB gene mutations in an Iranian population. A total of 30 non-related Iranian possible FH subjects were studied. Diagnosis of FH was based on the Dutch Lipid Clinic Network diagnostic criteria. All samples were initially tested for three common APOB gene mutations including R3500Q, R3500 W and R3531C using PCR-RFLP assay. Subsequently, promoter and coding region of the LDLR gene was screened by PCR-SSCP analysis and positive results were confirmed by DNA sequencing. Four previously reported polymorphisms 1413G > A, 1725C > T, 1773T > C and 2140 + 5G > A were found in ~17% (5/30) of population studied. Moreover, no variation was found in APOB gene. Our data indicated that LDLR and APOB gene mutations have not contribution to possible FH in Iranian population studied here. However, we examined three common APOB mutations and LDLR in only 30 patients, and to determine the role of these genes in developing FH in Iran, more FH samples and populations needed to be investigated for the mutations of the related genes.  相似文献   

Mechanism of Antidiabetic Action of Compound GII Purified from Fenugreek (<Emphasis Type="Italic">Trigonella foenum graecum</Emphasis>) Seeds     

D. Puri  K. M. Prabhu  G. Dev  S. Agarwal  P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》2011,26(4):335-346

To study the mechanism of action of water soluble compound GII purified from fenugreek (Trigonella foenum graecum) seeds which was shown earlier to have antidiabetic effect in the subdiabetic, moderately and severely diabetic rabbits. In rabbits (1–1.5 kg bw) diabetes was induced by intravenous injection of 80 mg/kg bw of alloxan. They were fed with GII at a dose of 50 mg/kg bw daily once in the morning for 15 days in the subdiabetic and moderately diabetic and 30 days in the severely diabetic rabbits. Serum total cholesterol (TC), triglycerides (TG), LDL + VLDL cholesterol [(LDL + VLDL)C], HDL cholesterol [(HDL)C], total tissue lipids, glycogen and enzymes of carbohydrate metabolism (glycolysis, gluconeogenesis, polyol pathway) hexokinase, glucokinase, pyruvate kinase, malic enzyme, glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, aldose reductase and sorbitol dehydrogenase and antioxidant enzymes glutathione peroxidase, glutathione reductase and superoxide dismutase were estimated. Liver and kidney function parameters were also estimated. Treatment with GII for 15 days in the subdiabetic and moderately diabetic rabbits and for 30 days in the severely diabetic rabbits (i) decreased the elevated lipids TC, TG, (LDL + VLDL)C and increased the decreased (HDL)C, (ii) decreased the elevated liver and heart total lipids, TC and TG, (iii) increased the decreased liver and muscle glycogen, (iv) increased the decreased hexokinase, glucokinase, pyruvate kinase, malic enzyme, glucose-6-phosphate dehydrogenase, superoxide dismutase, glutathione peroxidase, (v) decreased the increased glucose-6-phosphatase, sorbitol dehydrogenase, aldose reductase. Results thus show that treatment with GII compound purified from fenugreek seeds for 15 days in the subdiabetic and moderately diabetic and 30 days in the severely diabetic rabbits corrects the altered serum lipids, tissue lipids, glycogen, enzymes of glycolysis, gluconeogenesis, glycogen metabolism, polyol pathway and antioxidant enzymes. Histopathological abnormalities (fatty infiltration and other cellular changes) seen in the pancreas, liver, heart and kidneys were repaired after treatment with GII. In fact partially damaged pancreas was repaired. Liver and kidney function test results were normal in the GII treated animals indicating that GII treatment is safe and free from any side effects.  相似文献   

Diagnostic Dilemma of HbA1c Detection in Presence of a Hemoglobinopathy: A Case Report     

Bhat VS  Dewan KK  Krishnaswamy PR 《Indian journal of clinical biochemistry : IJCB》2011,26(1):91-95

We report a case of a diabetic, heterozygote with near normal hematology, marginally low level of hemoglobin A₂(HbA₂) having an increased level of hemoglobin F(HbF) that was pancellularly distributed among the red cells. BioRad DiaSTAT measurements gave a high glycated hemoglobin A1c(HbA1c) of 31.5% and the BioRad Variant analyzer recorded an HbA1c value which was very low, in discordance with the detected blood glucose levels. Flow cytometry and polymerization chain reaction (PCR) based studies were carried out which revealed the case to be that of the common hereditary persistence of fetal hemoglobin (HPFH)-3, an Asian Indian mutation. Fructosamine estimation and HbA1c by Boronate affinity chromatography were able to resolve the discordant value detected and was able to confirm the diabetes status. The case would have been a diagnostic dilemma, if reported without correlation.  相似文献   

BMD and Serum Intact Osteocalcin in Postmenopausal Osteoporosis Women     

Jagtap VR  Ganu JV  Nagane NS 《Indian journal of clinical biochemistry : IJCB》2011,26(1):70-73

India seems to have the highest prevalence of osteoporosis. With growing awareness of osteoporosis and its impact on life span especially in India, special attention is being paid to early detection, management and treatment of postmenopausal osteoporosis in women. Measurement of BMD and osteocalcin are of value in estimating bone turnover rates. The aim of this study is (1) to measure the specific, sensitive bone formation marker such as osteocalcin and BMD in postmenopausal osteoporosis women and postmenopausal non-osteoporosis women; (2) the follow up study to evaluate the impact of specific antiresorptive therapy (alendronate + calcium + vitamin D) regimen in postmenopausal osteoporosis by assaying osteocalcin and BMD. Sixty clinically diagnosed postmenopausal osteoporosis patients and 60 normal subjects (postmenopausal non-osteoporosis women) were recruited as control. Mean bone mineral density T score and Z score was significantly decreased (P < 0.001) in postmenopausal osteoporosis patients as compared to controls. Highly significant increase in the mean score of BMD—T score and Z score from baseline to post therapy of 3 months was observed in postmenopausal osteoporosis women. Serum osteocalcin levels were significantly increased (P < 0.001) as compared to control group. Serum osteocalcin levels were decreased significantly (P < 0.001) from baseline to post therapy of 3 months in postmenopausal osteoporosis women. BMD is the best quantifiable predictor of osteoporotic fracture and osteocalcin is specific, sensitive, promising, currently used marker for better prognosis of osteoporosis and for monitoring responses to antiresorptive therapy.  相似文献   

A Review on Salivary Genomics and Proteomics Biomarkers in Oral Cancer     

Franky D. Shah  Rasheedunnisa Begum  Bhairavi N. Vajaria  Kinjal R. Patel  Jayendra B. Patel  Shilin N. Shukla  Prabhudas S. Patel 《Indian journal of clinical biochemistry : IJCB》2011,26(4):326-334

Oral cancer has emerged as an alarming public health problem with increasing incidence and mortality rates all over the world. Therefore, the implementation of newer screening and early detection approaches are of utmost importance which could reduce the morbidity and mortality associated with this disease. Sensitive and specific biomarkers for oral cancer are likely to be most effective for screening, diagnosis, staging and follow-up for this dreaded malignancy. Unlike other deep cancers, oral cancer is located in oral cavity. Hence, the direct contact between saliva and oral cancer lesion makes the measurement of tumor markers in saliva an attractive alternative to serum and tissue testing. The DNA, RNA and protein molecules derived from the living cancer cells can be conveniently obtained from saliva. Thus, salivary biomarkers, a non-invasive alternative to serum and tissue-based biomarkers may be an effective modality for early diagnosis, prognostication and monitoring post therapy status. In the current post-genomic era, various technologies provide opportunities for high-throughput approaches to genomics and proteomics; which have been used to evaluate altered expressions of gene and protein targets in saliva of oral cancer patients. The emerging field of salivary biomarkers has great potentials to prove its clinical significance to combat oral cancer. Hence, we have reviewed importance of several salivary genomics and proteomics biomarkers for oral cancer.  相似文献   

The European university landscape: A micro characterization based on evidence from the Aquameth project   总被引：2，自引：0，他引：2  

Cinzia Daraio  Andrea BonaccorsiAldo Geuna  Benedetto LeporiLaurent Bach  Peter BogetoftMargarida F. Cardoso  Elena Castro-MartinezGustavo Crespi  Ignacio Fernandez de LucioHarold Fried  Adela Garcia-AracilAnnamaria Inzelt  Ben JongbloedGerhard Kempkes  Patrick LlerenaMireille Matt  Maria OlivaresCarsten Pohl  Tarmo RatyMaria J. Rosa  Cláudia S. SarricoLéopold Simar  Stig SlipersaeterPedro N. Teixeira  Philippe Vanden Eeckaut 《Research Policy》2011,40(1):148-164

This paper provides a new and systematic characterization of 488 universities, from 11 European countries: Finland, France, Germany, Hungary, Italy, Netherlands, Norway, Portugal, Spain, Switzerland and UK. Using micro indicators built on the integrated Aquameth database, we characterize the European university landscape according to the following dimensions: history/foundation of university, dynamics of growth, specialization pattern, subject mix, funding composition, offer profile and productivity.  相似文献   

Reliability modelling and analysis of a two unit continuous casting plant     

A.G. Mathew  S.M. Rizwan 《Journal of The Franklin Institute》2011,348(7):1488-1505

The aim of this paper is to present a reliability modeling and analysis of a two-unit system of a continuous casting (CC) plant. Real maintenance data of a steel production plant have been used for the purpose. Three types of failures were noted: repairable, replaceable and reconditioning/reinstallation. The two-unit system is operating in parallel, on full-reduced installed capacity. Upon the unit failure, an inspection is carried out to decide the type of maintenance task to be performed. Optimized reliability indices of the plant such as mean time to system failure, availability, expected busy periods of the repairman for various maintenance tasks, expected number of visits by the repairman, expected number of repairs, replacements and reconditioning/reinstallation are obtained. Semi-Markov processes and regenerative point techniques are used for the analysis. Profit incurred to the CC plant is also evaluated and related graphs are plotted to demonstrate the results.  相似文献   

Exact analysis for the identification of non-minimum phase processes     

P.K. Padhy  S. Majhi 《Journal of The Franklin Institute》2011,348(10):2734-2743

Analytical expressions are derived from a single symmetrical relay feedback test for the identification of open loop stable and unstable non-minimum phase processes. The second derivative of the limit cycle output helps in finding the time delay of the process. The derived expressions can be used to identify the process models with up to five unknowns. The effectiveness of the proposed identification method is verified through different simulation results.  相似文献   

New results on BIBO stability analysis for a class of neutral delay systems     

B. Wang  J. Wang  S.M. Zhong 《Journal of The Franklin Institute》2011,348(2):426-437

This paper studies bounded input bounded output (BIBO) stability for a class of neutral systems with time-varying delays. Based on Lyapunov method and linear matrix inequalities, some new BIBO stability criteria are established. The numerical simulation is made to demonstrate the effectiveness of the theoretical results obtained in this paper.  相似文献   

A simple design method for the cosine-modulated filter banks using weighted constrained least square technique   总被引：1，自引：0，他引：1  

A. Kumar  G.K. Singh  R.S. Anand 《Journal of The Franklin Institute》2011,348(4):606-621

This paper presents a simple and efficient design method for cosine-modulated filter banks with prescribed stopband attenuation, passband ripple, and channel overlap. The method casts the design problem as a linear minimization of filter coefficients such that their value at ω=π/2M is 0.707, which results in a simpler, more direct design procedure. The weighted constrained least squares technique is exploited for designing the prototype filter for cosine modulation (CM) filter banks. Several design examples are included to show the increased efficiency and flexibility of the proposed method over the exiting methods. An application of the proposed method is considered in the area of sub-band coding of the ECG and speech signals.  相似文献