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We introduce a method for improved dielectrophoretic (DEP) discrimination and separation of viable and nonviable yeast cells. Due to the higher cell wall permeability of nonviable yeast cells compared with their viable counterpart, the cross-linking agent glutaraldehyde (GLT) is shown to selectively cross-link nonviable cells to a much greater extent than viable yeast. The DEP crossover frequency (cof) of both viable and nonviable yeast cells was measured over a large range of buffer conductivities (22 μS∕cm–400 μS∕cm) in order to study this effect. The results indicate that due to selective nonviable cell cross-linking, GLT modifies the DEP cof of nonviable cells, while viable cell cof remains relatively unaffected. To investigate this in more detail, a dual-shelled oblate spheroid model was evoked and fitted to the cof data to study cell electrical properties. GLT treatment is shown to minimize ion leakage out of the nonviable yeast cells by minimizing changes in cytoplasm conductivity over a large range of ionic concentrations. This effect is only observable in nonviable cells where GLT treatment serves to stabilize the cell cytoplasm conductivity over a large range of buffer conductivity and allow for much greater differences between viable and nonviable cell cofs. As such, by taking advantage of differences in cell wall permeability GLT magnifies the effect DEP has on the field induced separation of viable and nonviable yeasts. 相似文献
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Joshua T. Weinhandl Bobbie S. Irmischer Zachary A. Sievert Kevin C. Fontenot 《Journal of sports sciences》2017,35(2):166-174
Limb dominance theory suggests that females tend to be more one-leg dominant and exhibit greater kinematic and kinetic leg asymmetries than their male counterparts, contributing to the increased risk of anterior cruciate ligament injury among female athletes. Thus, the purpose of this study was to examine the influences of sex and limb dominance on lower extremity joint mechanics during unilateral land-and-cut manoeuvres. Twenty-one women and 21 men completed land-and-cut manoeuvres on their dominant limb as well as their nondominant limb. Three-dimensional kinematics and kinetics were calculated bilaterally for the entire stance phase of the manoeuvre. Women performed land-and-cut manoeuvres with altered hip motions and loads as well as greater knee abduction at touchdown compared to men. Dominant limb land-and-cut manoeuvres where characterised by decreased hip flexion at touchdown as well as decreased hip flexion and adduction range of motion compared to nondominant land-and-cuts regardless of sex. The observed sex differences are consistent with previous research regarding mechanisms underlying the sex disparity in anterior cruciate ligament injury rates. However, observed differences regarding limb dominances appear somewhat arbitrary and did not suggest that the dominant or nondominant limb would be at increased risk of anterior cruciate ligament injury. 相似文献
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Keith C. Radley Roderick D. O'Handley Zachary C. Labrot 《Psychology in the schools》2015,52(4):363-378
Assessment in social skills training often utilizes procedures such as partial‐interval recording (PIR) and momentary time sampling (MTS) to estimate changes in duration in social engagements due to intervention. Although previous research suggests PIR to be more inaccurate than MTS in estimating levels of behavior, treatment analysis decisions have been found to be similar across procedures. To addresses limitations of previous studies that did not find substantial differences in treatment analysis decisions, PIR and MTS estimates were compared to those of continuous duration recording of social engagements to determine the effect on treatment interpretation. Five previously conducted social skills training data sets were coded using PIR, MTS, and duration recording. Treatment analysis interpretations using MTS were found to closely match those made using duration recording, but interpretations using PIR significantly overestimated the effectiveness of the intervention. Implications of findings are discussed in terms of assessment for social skills training in both research and applied settings. 相似文献
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Eleazar Vasquez III Angel Lopez Carrie Straub Selma Powell Tracy McKinney Zachary Walker Tiphanie Gonzalez Timothy A. Slocum Lee Mason Breda V. Okeeffe Peña L. Bedesem 《Learning disabilities research & practice》2011,26(2):84-93
Ethnic minority disproportionality has been a topic of extensive discussion and research for many years. In 1997, Artiles, Trent, and Kuan conducted a seminal review of the special education research literature to identify how often researchers report and disaggregate data in ways that would support conclusions about specific ethnic minority groups. These authors found alarmingly low rates of publication on identifiable minority groups. The purpose of this review is to replicate the work of Artiles et al. and extend this literature analysis to the subsequent 15‐year period (1995–2009). We found increases in the proportion of articles reporting ethnic minority information 15 years following the Artiles et al. publication. Discussion focuses on the gap in our knowledge of evidence‐based practices for ethnic minority students in special education. 相似文献
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Janice M. Y. Cheong Zachary M. Walker Kara Rosenblatt 《International Journal of Disability, Development & Education》2017,64(2):150-168
Mathematics is an important aspect of daily life. Basic numeracy skills are needed to accomplish everyday tasks. However, research regarding the relationship between cognitive ability, mental age, and basic numeracy skills for children with intellectual disability (ID) is scarce. This research study investigated the correlation between intellectual ability and the development of number sense and arithmetic abilities in individuals with ID. Thirty-two students from a special school for individuals with ID in Singapore participated in this research study. Based on the results, basic numeracy is more closely correlated to the mental ages of the students than their intelligence quotient. This finding will be discussed and suggestions for future research will be provided. 相似文献
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Joseph M. Currin Randolph D. Hubach André R. Durham Katherine E. Kavanaugh Zachary Vineyard Julie M. Croff 《Sex education》2017,17(6):667-681
The information shared in schools on sex education in the USA is highly variable depending on the state and sometimes city in which a student lives. Gay and bisexual students living in a socially conservative, primarily rural state such as Oklahoma often receive little information about sexual health information that pertains to their behaviours unless it is discussing the dangers of sex. Using a qualitative approach, 20 gay and bisexual men currently residing in Oklahoma completed semi-structured interviews about their experiences with sex education and alternate ways to compensate for lack of information provided. Participants noted an awareness of the lack of meaningful sex education or if it was offered, a primary focus on abstinence-only until marriage heteronormative curricula. Participants described various ways they compensated for lack of sex education including accessing pornography and information from the internet. With the current socio-political environment of Oklahoma, it is unlikely wholesale changes to the sex education curricula can be made. The importance of public health professionals using the internet and peer educators to deliver inclusive and informative sexual health information remains salient and viable for men who reside in socio-politically conservative areas like Oklahoma. 相似文献