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Sun HC Xue FM Qian K Fang HX Qiu HL Zhang XY Yin ZH 《Journal of Zhejiang University. Science. B》2006,7(4):324-330
To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme （hLYZ） cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400μg of the p215C3LYZ vector, over 2.0 μg/ml ofrhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhL YZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hL YZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis. 相似文献
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情商水平的高低对一个人能否取得成功有着重大的影响,其作用有时甚至要超过智力水平.然而,当代大学生并投有从思想上意识到情商培养对自身发展的重要性以及必要性,导致了当代大学生群体情商普遍偏低现状的出现.立足于当代大学生自我激励和心理调节能力较差,情绪波动大,容易被情绪左右,人际交往能力不足等现状,结合大学生由情商偏低引起的就业瓶颈现象,从树立正确的人生观和价值观、学会认识和调控自身及他人情绪以及广交人脉等角度出发,提出了大学生的情商培养方案. 相似文献
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INTRODUCTION Lipase (EC 3.1.1.3) is an important hydrolysis enzyme (Jaeger et al., 1994) excreted to the envi-ronment by plant roots and microorganisms. Many pollutants such as organophosphorus pesticides and synthetic polyesters and polyamides can be degraded by lipase (Monroy-Noyola et al., 1999; Marten et al., 2003). In soil, enzymatic reactions occur in a het-erogeneous rather than homogeneous environment (Huang et al., 1998). Catalytic function of lipase, an extracelluar enzyme, … 相似文献
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Objective： To investigate the allocation and management of large medical equipment （LME） in Xuzhou, Jiangsu Province, China, in order to make the best use of LME to meet the medical needs of local people. Methods： The research collected data from all hospitals that have LME in Xuzhou using questionnaire; 38 （97.4%） hospitals returned the questionnaire. Results： In Xuzhou, there are a total of 71 pieces of LME, each serving 126 600 people in an area of 163 km^2. Sixty-two percent of them are allocated in urban areas, with Gini coefficient at 0.52, indicating imbalance and biased allocation of LME. Conclusion： The allocation of LME in Xuzhou is out of control and unfairly allocated. 相似文献
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Chylothorax is an uncommon disease where fatty fluid accumulates within the chest cavity. Conservative management, including repeated thoracentesis or pleurodesis, seems to be suitable to most cases. Herein, we present a case of efficacious pleurodesis by intrapleural injection of Sapylin, a streptococcus preparation, for the treatment of chylothorax. A 52-year-old non-smoking female farmer was diagnosed as idiopathic chylothorax after we ruled out possible causes including chest trauma, lymphoma, lung cancer, filariasis, tuberculosis, and etc. Two-time intra-thoracic injection of 3 Klinische Einheit （KE） Sapylin achieved rapid and effective control of chylothorax with no severe side effects. Sapylin may facilitate pleurodesis by producing a strong inflammatory response. 相似文献
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RsrhonthWynhoioMrinLisurSportsMngmnt—sStuyoZhoushnrhiplgo张腾ZHNGTng 《四川体育科学》2014,(1):96-98,117
通过问卷调查、专家访谈法并结合当前舟山地区政治经济背景,在分析了海洋休闲体育开展现状的基础上,提出了如何有效进行管理以改进海洋休闲体育在本地区的发展思路、方法、策略,旨在探讨海洋休闲体育管理的路径,并提出了海洋休闲体育发展中政府作用的重点与方向的相关建议,为海洋休闲体育的发展提供实践指导。 相似文献