Membrane-integrated microfluidic device for high-resolution live cell imaging     

Epshteyn AA  Maher S  Taylor AJ  Holton AB  Borenstein JT  Cuiffi JD 《Biomicrofluidics》2011,5(4):46501-465016

The design and fabrication of a membrane-integrated microfluidic cell culture device (five layers,≤500 μm total thickness) developed for high resolution microscopy is reported here. The multi-layer device was constructed to enable membrane separated cell culture for tissue mimetic in vitro model applications and pharmacodynamic evaluation studies. The microdevice was developed via a unique combination of low profile fluidic interconnect design, substrate transfer methodology, and wet silane bonding. To demonstrate the unique high resolution imaging capability of this device, we used oil immersion microscopy to image stained nuclei and mitochondria in primary hepatocytes adhered to the incorporated membrane  相似文献   

Effect of post-match cold-water immersion on subsequent match running performance in junior soccer players during tournament play   总被引：2，自引：1，他引：1  

Rowsell GJ  Coutts AJ  Reaburn P  Hill-Haas S 《Journal of sports sciences》2011,29(1):1-6

In this study, we investigated the effects of two hydrotherapy interventions on match running performance and perceptual measures of fatigue and recovery during a 4-day soccer tournament. Twenty male junior soccer players were assigned to one of two treatment groups and undertook either cold-water immersion (5 × 1 min at 10 °C) or thermoneutral water immersion (5 × 1 min at 34 °C) after each match. High-intensity running distance (>15 km · h?1) and total distance covered, time spent in low (<80% maximum heart rate), moderate (80-90% maximum heart rate), and high (>90% maximum heart rate) heart rate zones, and rating of perceived exertion (RPE) were recorded for each match. Perceptions of general fatigue and leg soreness were recorded approximately 22 h after each match. There were decreases in both groups across the 4-day tournament for high-intensity running distance (P = 0.006, Cohen's d = 0.63), total distance run (P < 0.001, d = 0.90), time in high heart rate zone (P = 0.003, d = 0.90), and match RPE (P = 0.012, d = 0.52). Cold-water immersion was more effective than thermoneutral immersion for reducing the perception of leg soreness (P = 0.004, d = -0.92) and general fatigue (P = 0.007, d = -0.91), ameliorating the decrement in total distance run (P = 0.001, d = 0.55), and maintaining time in the moderate heart rate zone (P = 0.01, d = 1.06). In conclusion, cold-water immersion mediates the perceptions of fatigue and recovery and enhances the restoration of some match-related performance measures during a 4-day tournament.  相似文献   

The Influence of Rurality and Parental Affect on Kindergarten Children's Social and Behavioral Functioning     

Susan M. Sheridan  Natalie A. Koziol  Brandy L. Clarke  Kristin M. Rispoli  Michael J. Coutts 《Early education and development》2014,25(7):1057-1082

Research Findings: Children's early academic achievement is supported by positive social and behavioral skills, and difficulties with these skills frequently gives way to underachievement. Social and behavioral problems often arise as a product of parent–child interactional patterns and environmental influences. Few studies have examined the role of a salient aspect of children's environments, community locale, in the relationship between parenting practices and child outcomes. Using a large, nationally representative sample, we examined whether preschool parenting practices and children's social-behavioral skills in kindergarten were related to geographic setting (rural vs. city, suburban, and town). Results indicated that rural children experienced greater difficulties with parent-reported externalizing behaviors. Furthermore, rural parents displayed less emotional support than parents in other settings. Preschool parenting behaviors were associated with social skills and behavior problems in kindergarten, as reported by both parents and teachers. Parents' emotional supportiveness was found to account for the relationship between geographic setting and parent-reported children's social skills, such that rural parents who provided less emotional support had children with lower social skills in kindergarten. Practice or Policy: Findings of this research indicate that rural children may face particular risk for behavioral issues and highlight the need for increased behavioral supports in rural communities. Moreover, our results suggest that interventions designed to promote parents' support of children's emotions may have particular utility for rural families.  相似文献   

A Behind‐the‐Scenes Guide to School‐Based Research     

Benjamin D. Plummer  Brian M. Galla  Amy S. Finn  Sarah D. Patrick  David Meketon  Julia Leonard  Calvin Goetz  Elizabeth Fernandez‐Vina  Sara Bartolino  Rachel E. White  Angela L. Duckworth 《Mind, Brain, and Education》2014,8(1):15-20

Schools are an important context for both basic and applied scientific research. Unlike the laboratory, however, the physical and social conditions of schools are not under the exclusive control of scientists. In this article, we liken collecting data in schools to putting on a theatrical production. We begin by describing the large cast of characters whose collaborative efforts make school‐based research possible. Next, we address the critics, including the university Institutional Review Board (IRB) and school administrators, whose feedback often improves the final study design. We then turn our attention to set building, stage directions, and rehearsals—key steps in the iterative process of refining study procedures. We end with a discussion of the day of data collection itself and activities that take place after the curtain drops. Throughout, we make recommendations based on our recent experience collecting data at several high schools.  相似文献   

Use of the information budget     

Angela R. Haygrath-Jackson 《Learned Publishing》1989,2(2):78-86

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Domestic Dilemmas 1     

Angela Fisher 《English in Education》1990,24(1):40-40

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Some Queries About Examiners' Criteria and Students' Results Raised by the 'A' Level English Literature Examination     

Angela Dale 《English in Education》1972,6(1):63-74

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The wardenly role: the academic presence in university residence     

Barry Pashley  Angela Shepherd 《Higher Education Quarterly》1975,29(3):332-352

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Evaluation for growth: growth of an evaluation; a case study of a local TVEI evaluation     

Angela Fraser 《Cambridge Journal of Education》1986,16(2):84-93

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Learning set-ups     

Doris Allen  Angela Collins 《Early Childhood Education Journal》1977,4(5):34-35

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