A tapered channel microfluidic device for comprehensive cell adhesion analysis, using measurements of detachment kinetics and shear stress-dependent motion     

Rupprecht P  Golé L  Rieu JP  Vézy C  Ferrigno R  Mertani HC  Rivière C 《Biomicrofluidics》2012,6(1):14107-1410712

We have developed a method for studying cellular adhesion by using a custom-designed microfluidic device with parallel non-connected tapered channels. The design enables investigation of cellular responses to a large range of shear stress (ratio of 25) with a single input flow-rate. For each shear stress, a large number of cells are analyzed (500–1500 cells), providing statistically relevant data within a single experiment. Besides adhesion strength measurements, the microsystem presented in this paper enables in-depth analysis of cell detachment kinetics by real-time videomicroscopy. It offers the possibility to analyze adhesion-associated processes, such as migration or cell shape change, within the same experiment. To show the versatility of our device, we examined quantitatively cell adhesion by analyzing kinetics, adhesive strength and migration behaviour or cell shape modifications of the unicellular model cell organism Dictyostelium discoideum at 21 °C and of the human breast cancer cell line MDA-MB-231 at 37 °C. For both cell types, we found that the threshold stresses, which are necessary to detach the cells, follow lognormal distributions, and that the detachment process follows first order kinetics. In addition, for particular conditions’ cells are found to exhibit similar adhesion threshold stresses, but very different detachment kinetics, revealing the importance of dynamics analysis to fully describe cell adhesion. With its rapid implementation and potential for parallel sample processing, such microsystem offers a highly controllable platform for exploring cell adhesion characteristics in a large set of environmental conditions and cell types, and could have wide applications across cell biology, tissue engineering, and cell screening.  相似文献   

The planarity of the stickface motion in the field hockey hit     

Willmott AP  Dapena J 《Journal of sports sciences》2012,30(4):369-377

The field hockey hit is an important but poorly understood stroke. In this study, we investigated the planarity of the stickface motion during the downswing to better characterize the kinematics and to assess the suitability of planar pendulum models for simulating the hit. Thirteen experienced female field hockey players were filmed executing hits with a single approach step, and the kinematics of the centre of the stickface were measured. A method was developed for identifying how far back from impact the stickface motion was planar. Orthogonal regression was used to fit least-squares planes to the stickface path during sections of the downswing of varying length, with each section ending at impact. A section was considered planar if the root mean square residual between the stickface path and the fitted plane was less than 0.25% of the distance travelled by the stickface during that period. On average, the stickface motion was planar for the last 83 ± 12% of its downswing path, with the length of the planar section ranging from 1.85 m to 2.70 m. The suitability of a planar model for the stickface motion was supported, but further investigation of the stick and arm kinematics is warranted.  相似文献   

Anthropometric profiles of elite older triathletes in the Ironman Brazil compared with those of young Portuguese triathletes and older Brazilians     

Silva DA  Benedetti TR  Ferrari EP  Meurer ST  Antes DL  Silva AM  Santos DA  Matias CN  Sardinha LB  Vieira F  Petroski EL 《Journal of sports sciences》2012,30(5):479-484

The objective of this study was to describe the anthropometric profiles of elite older triathletes participating in the 2009 Ironman Brazil and to compare their somatotype, anthropometric and body composition characteristics with those of elite young triathletes and older non-athletes. The sample consisted of 64 males, divided into three groups: (1) older triathletes (n = 17), (2) young triathletes (n = 24), and (3) older non-athletes (n = 23). Somatotype was analysed according to the Carter and Heath ( 1990 ) method. Body mass index, sum of four skinfolds, percentage body fat, body fat mass, and fat-free mass were also estimated. Older non-athletes had higher endomorphy, sum of four skinfolds, fat percentage, and body fat mass than the athletes. Older athletes who participated in the Ironman and elite young triathletes had higher fat-free mass than older non-athletes. Older triathletes and older non-athletes were considered mainly endomorphic mesomorphs and young triathletes mainly ectomorphic mesomorphs. The findings that characterize the anthropometric profile of athletes in this sports modality could be used as a reference.  相似文献   

A collaborative filtering similarity measure based on singularities     

Jesús Bobadilla  Fernando OrtegaAntonio Hernando 《Information processing & management》2012

Recommender systems play an important role in reducing the negative impact of information overload on those websites where users have the possibility of voting for their preferences on items. The most normal technique for dealing with the recommendation mechanism is to use collaborative filtering, in which it is essential to discover the most similar users to whom you desire to make recommendations. The hypothesis of this paper is that the results obtained by applying traditional similarities measures can be improved by taking contextual information, drawn from the entire body of users, and using it to calculate the singularity which exists, for each item, in the votes cast by each pair of users that you wish to compare. As such, the greater the measure of singularity result between the votes cast by two given users, the greater the impact this will have on the similarity. The results, tested on the Movielens, Netflix and FilmAffinity databases, corroborate the excellent behaviour of the singularity measure proposed.  相似文献   

Fault reconstruction using a LPV sliding mode observer for a class of LPV systems     

Halim Alwi  Christopher Edwards  Andrés Marcos 《Journal of The Franklin Institute》2012,349(2):510-530

This paper proposes a new sliding mode observer for fault reconstruction, applicable for a class of linear parameter varying (LPV) systems. Observer schemes for actuator and sensor fault reconstruction are presented. For the actuator fault reconstruction scheme, a virtual system comprising the system matrix and a fixed input distribution matrix is used for the design of the observer. The fixed input distribution matrix is instrumental in simplifying the synthesis procedure to create the observer gains to ensure a stable closed-loop reduced order sliding motion. The ‘output error injection signals’ from the observer are used as the basis for reconstructing the fault signals. For the sensor fault observer design, augmenting the LPV system with a filtered version of the faulty measurements allows the sensor fault reconstruction problem to be posed as an actuator fault reconstruction scenario. Simulation tests based on a high-fidelity nonlinear model of a transport aircraft have been used to demonstrate the proposed actuator and sensor FDI schemes. The simulation results show their efficacy.  相似文献   

Generation of virtual models of cultural heritage     

Amparo Núñez Andrés  Felipe Buill Pozuelo  Joaquín Regot Marimón  Andrés de Mesa Gisbert 《Journal of Cultural Heritage》2012,13(1):103-106

Different techniques and tools currently exist to generate three-dimensional models of small elements, buildings and cities. Apart from being easier to interpret than two-dimensional drawings, these models facilitate data necessary for reconstruction projects, preservation or rehabilitation of the architectural or archaeological heritage. Traditional surveying only enables us to obtain discrete information of the characteristic lines that define each surface, edge, slope change etc. whereas work with massive capture techniques allows us to obtain continuous points of the surfaces. Photogrammetry or laser scanning combined with conventional photography provides information of the building in addition to its geometry. We carried out a review of the different techniques showing the advantages and disadvantages of each technique as well as the information that can be obtained by applying these techniques to the survey of the great Gate of Antioch in the town of Aleppo, declared a World Heritage Site by UNESCO in 1986, in Syria.  相似文献   

Fingerspelling and sign language as alternative codes for reading and writing words for Chilean deaf signers     

Puente A  Alvarado JM  Herrera V 《American annals of the deaf》2006,151(3):299-310

The study examined the role of sign language and fingerspelling in the development of the reading and writing skills of deaf children and youth. Twenty-six deaf participants (13 children, 13 adolescents), whose first language was Chilean Sign Language (CHSL), were examined. Their dactylic abilities were evaluated with tasks involving the reading and writing of dactylic and orthographic codes. The study included three experiments: (a) the identification of Chilean signs and fingerspelled words, (b) the matching of fingerspelled words with commercial logos, and (c) the decoding of fingerspelled words and the mapping of these words onto the writing system. The results provide convergent evidence that the use of fingerspelling and sign language is related to orthographic skills. It is concluded that fingerspelling can facilitate the internal representation of words and serve as a supporting mechanism for reading acquisition.  相似文献   

Δ-distance: A family of dissimilarity metrics between images represented by multi-level feature vectors     

Marta Rukoz  Maude Manouvrier  Geneviève Jomier 《Information Retrieval》2006,9(6):633-655

This article presents the Δ-distance, a family of distances between images recursively decomposed into segments and represented by multi-level feature vectors. Such a structure is a quad, a quin or a nona-tree resulting from a fixed and arbitrary image partition or from an image segmentation process. It handles positional information of image features (e.g. color, texture or shape). Δ-distance is the generalized form of dissimilarity measures between multi-level feature vectors. Using different weights on tree nodes and different distances between nodes, distances between trees or visual similarity between images can be computed based on the general definition of Δ. In this article, we present three Δ-based distance families: two families of distances between tree structures, called -distance( for Tree) and -distance ( for Segment), and a family of visual distances between images, called ( for Visual). The -distance visually compares two images using their tree representation and the other two distances compare the tree structures resulting from image segmentation. Moreover, we show how existing distances between multi-level feature vectors appear to be particular cases of the Δ-distance  相似文献   

Hirsch-type characteristics of the tail of distributions. The generalised h-index     

Wolfgang Glänzel  András Schubert 《Journal of Informetrics》2010,4(1):118-123

In this paper a generalisation of the h-index and g-index is given on the basis of non-negative real-valued functionals defined on subspaces of the vector space generated by the ordered samples. Several Hirsch-type measures are defined and their basic properties are analysed. Empirical properties are illustrated using examples from the micro- and meso-level. Among these measures, the h-index proved the most, the arithmetic and geometric g-indices, the least robust measures. The μ-index and the harmonic g-index provide more balanced results and are still robust enough.  相似文献   

Flow Injection Amperometric Evaluation of Trolox Equivalent Antioxidant Capacity of Chocolates with Different Cocoa Content at a Boron-Doped Diamond Electrode     

Tahir Arbneshi  Arbër Frangu  Michaela Frühbauerov  Libor ervenka  Liridon Berisha  Kurt Kalcher  Milan Sýs 《Food Technology and Biotechnology》2021,59(2):194

  相似文献