Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design     

Leticia I. RAMíREZ-CAVAZOS  Charles JUNGHANNS  Rakesh NAIR  Diana L. CáRDENAS-CHáVEZ  Carlos HERNáNDEZ-LUNA  Spiros N. AGATHOS  Roberto PARRA 《Journal of Zhejiang University. Science. B》2014,15(4):343-352

研究目的：优化获得血红密孔菌（P.sanguineus）的最佳培养基组成,提高耐热漆酶的产量。创新要点：获得了目前文献报道的最高水平的漆酶活力。研究方法：通过单因素试验研究了不同培养基（番茄汁、麦麸、麦芽提取物和葡萄糖细菌蛋白胨培养基）和不同组合诱导剂（大豆油、阿魏酸、没食子酸、二甲基苯胺、酸性蓝62和活性蓝19分别与硫酸铜组合诱导剂）对P.sanguineus产耐热漆酶的影响。在此基础上采用中心组合试验设计,进一步研究了番茄汁培养基结合硫酸铜和大豆油组合诱导剂对P.sanguineus产耐热漆酶的影响。利用SAS10.0和响应面分析方法对试验结果进行了统计分析和建立回归模型。重要结论：通过中心组合设计优化得出P.sanguineus产耐热漆酶的最优培养基条件：以36.8%番茄汁为培养基,以3 mmol/L硫酸铜和1%大豆油作为组合诱导剂。该条件下在10 L搅拌槽生物反应器中漆酶活力达到了143 000 IU/L（2,2＇-联氮双（3-乙基苯并噻唑啉-6-磺酸）为底物,pH值为3.0）。  相似文献   

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勒花?拾.野尊i-安徒话象自清散文精选茶—花女_|—.I.._ 《中学生阅读(初中版)》2014,(8):66

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街角小店的情怀     

I’m  ViVi 《当代职校生》2014,(7):94-94

各种便利店越开越晚,越开越多,种类也越来越丰富。而我偏爱街角十字路口的小店。东西不多,价格便宜,有时候还有厚厚一层灰。老板不好意思地拿帕子擦干净。再附赠一张餐巾纸。这便是印象中充满人情味的小卖部。  相似文献   

Lay perceptions of collectives at the outbreak of the H1N1 epidemic: heroes, villains and victims     

Wagner-Egger P  Bangerter A  Gilles I  Green E  Rigaud D  Krings F  Staerklé C  Clémence A 《Public understanding of science (Bristol, England)》2011,20(4):461-476

Lay perceptions of collectives (e.g., groups, organizations, countries) implicated in the 2009 H1N1 outbreak were studied. Collectives serve symbolic functions to help laypersons make sense of the uncertainty involved in a disease outbreak. We argue that lay representations are dramatized, featuring characters like heroes, villains and victims. In interviews conducted soon after the outbreak, 47 Swiss respondents discussed the risk posed by H1N1, its origins and effects, and protective measures. Countries were the most frequent collectives mentioned. Poor, underdeveloped countries were depicted as victims, albeit ambivalently, as they were viewed as partly responsible for their own plight. Experts (physicians, researchers) and political and health authorities were depicted as heroes. Two villains emerged: the media (viewed as fear mongering or as a puppet serving powerful interests) and private corporations (e.g., the pharmaceutical industry). Laypersons' framing of disease threat diverges substantially from official perspectives.  相似文献   

Manipulating particle trajectories with phase-control in surface acoustic wave microfluidics     

Orloff ND  Dennis JR  Cecchini M  Schonbrun E  Rocas E  Wang Y  Novotny D  Simmonds RW  Moreland J  Takeuchi I  Booth JC 《Biomicrofluidics》2011,5(4):44107-441079

We present a 91 MHz surface acoustic wave resonator with integrated microfluidics that includes a flow focus, an expansion region, and a binning region in order to manipulate particle trajectories. We demonstrate the ability to change the position of the acoustic nodes by varying the electronic phase of one of the transducers relative to the other in a pseudo-static manner. The measurements were performed at room temperature with 3 μm diameter latex beads dispersed in a water-based solution. We demonstrate the dependence of nodal position on pseudo-static phase and show simultaneous control of 9 bead streams with spatial control of −0.058 μm/deg ± 0.001 μm/deg. As a consequence of changing the position of bead streams perpendicular to their flow direction, we also show that the integrated acoustic-microfluidic device can be used to change the trajectory of a bead stream towards a selected bin with an angular control of 0.008 deg/deg ± 0.000(2) deg/deg.  相似文献   

Dielectrophoretic manipulation of ribosomal RNA     

Giraud G  Pethig R  Schulze H  Henihan G  Terry JG  Menachery A  Ciani I  Corrigan D  Campbell CJ  Mount AR  Ghazal P  Walton AJ  Crain J  Bachmann TT 《Biomicrofluidics》2011,5(2):24116

The manipulation of ribosomal RNA (rRNA) extracted from E. coli cells by dielectrophoresis (DEP) has been demonstrated over the range of 3 kHz-50 MHz using interdigitated microelectrodes. Quantitative measurement using total internal reflection fluorescence microscopy of the time dependent collection indicated a positive DEP response characterized by a plateau between 3 kHz and 1 MHz followed by a decrease in response at higher frequencies. Negative DEP was observed above 9 MHz. The positive DEP response below 1 MHz is described by the Clausius-Mossotti model and corresponds to an induced dipole moment of 3300 D with a polarizability of 7.8×10(-32) F m(2). The negative DEP response above 9 MHz indicates that the rRNA molecules exhibit a net moment of -250 D, to give an effective permittivity value of 78.5 ε(0), close to that of the aqueous suspending medium, and a relatively small surface conductance value of ～0.1 nS. This suggests that our rRNA samples have a fairly open structure accessible to the surrounding water molecules, with counterions strongly bound to the charged phosphate groups in the rRNA backbone. These results are the first demonstration of DEP for fast capture and release of rRNA units, opening new opportunities for rRNA-based biosensing devices.  相似文献   

道德哲学对道德教育的贡献     

Chazan B.I  Soltis J.F.  戚万学 《上海教育科研》1993,(1)

当代道德教育的最显著特征就是说做不一。尽管对教育目的的大量正式陈述都断言,通过公共教育培养在道德上负责的公民乃是教育的核心,但事实上,能够认真、系统地对待道德教育的学校並不多;尽管那些市民、政治和教育领袖们口口声声说,教育在传递当今世界复杂的生活所必需的“道德和精神价值”方面起重要作用,但是为这种价值教育作出正式规定的教育体制也还毕竟是少数;人们常说教师乃是专少年品格形成的重要力量,然而,经过旨在提高道德教育工作者效能的训练的教师实为数寥寥。总之,在官方语言和当代实际的道德教育实践之间存在一种令人不安的不一致。即使我们不否认那些讲“官话”的人的诚意,有  相似文献   

透皮治疗不用打针吃药     

Wick.  I 郭永春 《科技新时代》1997,(2):28-31

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制定英国国家情报政策的迫切性     

Malley  I 董安民 《国外情报科学》1991,(4):59-62,46

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创新的六种方法     

Robert.I.Sutton 《技术创新》2004,(1):7-9

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