Single-cell attachment and culture method using a photochemical reaction in a closed microfluidic system     

Jang K  Xu Y  Tanaka Y  Sato K  Mawatari K  Konno T  Ishihara K  Kitamori T 《Biomicrofluidics》2010,4(3):32208

Recently, interest in single cell analysis has increased because of its potential for improving our understanding of cellular processes. Single cell operation and attachment is indispensable to realize this task. In this paper, we employed a simple and direct method for single-cell attachment and culture in a closed microchannel. The microchannel surface was modified by applying a nonbiofouling polymer, 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, and a nitrobenzyl photocleavable linker. Using ultraviolet (UV) light irradiation, the MPC polymer was selectively removed by a photochemical reaction that adjusted the cell adherence inside the microchannel. To obtain the desired single endothelial cell patterning in the microchannel, cell-adhesive regions were controlled by use of round photomasks with diameters of 10, 20, 30, or 50 μm. Single-cell adherence patterns were formed after 12 h of incubation, only when 20 and 30 μm photomasks were used, and the proportions of adherent and nonadherent cells among the entire UV-illuminated areas were 21.3%±0.3% and 7.9%±0.3%, respectively. The frequency of single-cell adherence in the case of the 20 μm photomask was 2.7 times greater than that in the case of the 30 μm photomask. We found that the 20 μm photomask was optimal for the formation of single-cell adherence patterns in the microchannel. This technique can be a powerful tool for analyzing environmental factors like cell-surface and cell-extracellular matrix contact.  相似文献   

Significant life experiences affect environmental action: a confirmation study in eastern Taiwan     

Shih‐Jang Hsu 《Environmental Education Research》2009,15(4):497-517

Two field studies form the basis of this article. The major purposes of Study 1 were to examine significant life experiences affecting the cultivation of environmental activists in eastern Taiwan, and to reconstruct the life paths followed by those active people who engaged in effective environmental action. 40 usable autobiographical memories were collected and content‐analysed to derive 17 significant life experiences. Based on the 17 accounts, a quantitative questionnaire of 24 items on significant life experiences variables was developed for Study 2. Four hundred and thirty valid questionnaires were analysed. Eighty two respondents with a high level of environmental action were determined to be environmental activists, and 153 with a low level of environmental action were determined to be people apathetic towards environmental protection. It was found that the significant life experiences identified in Study 1 could effectively distinguish environmentally committed people from those apathetic to environmental protection. Study 2 also found that 54.6% of the variances in environmental actions could be explained by the significant life experiences.  相似文献   

试论手机媒体对新闻传播活动的影响   总被引：3，自引：0，他引：3  

周怡  姜岩 《新闻界》2009,(1):76-78

手机改变了新闻摄影与摄像,将大批受众纳入到新闻图片、影像采集者的行列中.手机给记者采访带来巨大的方便,对新闻制作提出新的要求.手机为受众带来新的媒介体验,使用户同传者的身份界限变得模糊,成为用户与新闻节目互动的工具.  相似文献   

Disability in higher education: a position paper     

King LH  Aguinaga N  O'Brien C  Young W  Zgonc K 《American annals of the deaf》2010,155(3):386-391

Leadership in education, particularly special education, implies both knowledge and application of professional behaviors, decisions, and ethics within the field. University students look for best practices in education to be modeled by faculty; this, in turn, underscores a fundamental belief in inclusionary principles. Such modeling is important on every level in teacher education programs, particularly special education programs. The higher a student's level (i.e., undergraduate vs. doctoral), the more notice that student will take of disparities between rhetoric and actions. While reflective practices are explicitly taught in teacher preparation programs to enable professionals to reflect on their own personal and professional practices, teacher education faculty need to employ the same strategy to ensure that they are indeed modeling the practices they teach. This position paper documents the impressions of a doctoral cohort in special education in terms of inclusionary practices demonstrated in a real-life context.  相似文献   

TEACHING TO UNLEARN COMMUNITY IN ORDER TO MAKE A CLAIM TO COMMUNITY     

Duck‐Joo Kwak 《Educational theory》2010,60(4):405-417

In this essay Duck‐Joo Kwak explores a moral perfectionist approach to citizenship education, which is distinct from liberal and communitarian models. One of educational challenges to this approach is how to cultivate our students' sense of membership, which is shaped by a thick sense of the good life, while being not merely compatible with but open to the pluralist perspective. Kwak maintains that what is required for this form of membership to society or community is our future citizens' ability to engage in highly self‐reflexive philosophical reflection on the human condition; such reflection gives them the skills necessary to live up to the tension between different selves of diverse origins or within a divided self. Examining Stanley Cavell's view of political education as an exemplar of the moral perfectionist approach to citizenship education, Kwak shows how the practice of his ordinary language philosophy can be a good way to cultivate this ability by teaching us how to “speak for others” by way of “speaking for oneself.”  相似文献   

A destination development by building a brand image and sport event tourism: a case of Sport City USA     

Young Hoon Kim  Hongxin Li  John Nauright 《Sport in Society》2018,21(8):1196-1203

Abstract

While visitors look for a complete ‘experience’ and select travel destinations on the basis of the total set of destination attributes, individual suppliers that operate independently usually market offer in fragmented pieces. A destination brand with strong equity leads to greater commitment in the form of loyalty and willingness to revisit the destination. As one of the key components for destination characteristics, the sports industry has rapidly developed in recent years. Two cities were discussed as the known for sport city case and Frisco, Texas was examined for the current study. The results of this study show that the economic impact is significant for a destination. Some critical factors (e.g. sustainability) should be discussed in future study.  相似文献   

Sixth Grade Students’ Content-Specific Competencies and Challenges in Learning the Seasons Through Modeling     

Ji Young Sung  Phil Seok Oh 《Research in Science Education》2018,48(4):839-864

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Sharing practices,but what is the story? Exploring award-winning teachers’ conceptions of teaching     

Mark Gan Joo Seng  Johan Geertsema 《高等教育研究与发展》2018,37(2):254-269

This paper critically considers the attempt by a research-intensive university in Asia to draw on and make public the practices of teaching award winners in different disciplines through the use of narrative vignettes. More specifically, this paper examines what the vignettes suggest about the connection between award-winning teachers’ conceptions of teaching (CT) on the one hand, and scholarly investigations of practice on the other, and to what extent the vignettes meet the aim of using the scholarship of teaching and learning as an instrument of effective academic development. Content analysis of the vignettes revealed that award-winning teachers’ CT tend to focus predominantly on enhancing learning, followed by encouraging collective knowledge creation and transmission of knowledge. A two-dimensional framework was also used to analyse the vignettes along a tacit-integrated continuum and within an intuitive-reflective frame of inquiry. The findings are discussed in relation to teachers’ perception of their impact on students’ learning and ways to enhance their own practice, with implications for teaching awards, documentation, and dissemination of high-level teaching achievement.  相似文献   

Poly(vinyl alcohol)-heparin biosynthetic microspheres produced by microfluidics and ultraviolet photopolymerisation     

Cara Young  Kester Rozario  Christophe Serra  Laura Poole-Warren  Penny Martens 《Biomicrofluidics》2013,7(4)

Biosynthetic microspheres have the potential to address some of the limitations in cell microencapsulation; however, the generation of biosynthetic hydrogel microspheres has not been investigated or applied to cell encapsulation. Droplet microfluidics has the potential to produce more uniform microspheres under conditions compatible with cell encapsulation. Therefore, the aim of this study was to understand the effect of process parameters on biosynthetic microsphere formation, size, and morphology with a co-flow microfluidic method. Poly(vinyl alcohol) (PVA), a synthetic hydrogel and heparin, a glycosaminoglycan were chosen as the hydrogels for this study. A capillary-based microfluidic droplet generation device was used, and by varying the flow rates of both the polymer and oil phases, the viscosity of the continuous oil phase, and the interfacial surface tension, monodisperse spheres were produced from ∼200 to 800 μm. The size and morphology were unaffected by the addition of heparin. The modulus of spheres was 397 and 335 kPa for PVA and PVA/heparin, respectively, and this was not different from the bulk gel modulus (312 and 365 for PVA and PVA/heparin, respectively). Mammalian cells encapsulated in the spheres had over 90% viability after 24 h in both PVA and PVA/heparin microspheres. After 28 days, viability was still over 90% for PVA-heparin spheres and was significantly higher than in PVA only spheres. The use of biosynthetic hydrogels with microfluidic and UV polymerisation methods offers an improved approach to long-term cell encapsulation.  相似文献   

The origins of the modern olympics: a new version     

David C. Young 《国际体育史杂志》2013,30(3):271-300

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