A simple and inexpensive enteric-coated capsule for delivery of acid-labile macromolecules to the small intestine     

Darren S. Miller  Anne Michelle Parsons  John Bresland  Paul Herde  Duc Minh Pham  Angel Tan  Hung-yao Hsu  Clive A. Prestidge  Tim Kuchel  Rezaul Begg  Syed Mahfuzul Aziz  Ross N. Butler 《Journal of Zhejiang University. Science. B》2015,16(7):586-592

Understanding the ecology of the gastrointestinal tract and the impact of the contents on the host mucosa is emerging as an important area for defining both wellness and susceptibility to disease. Targeted delivery of drugs to treat specific small intestinal disorders such as small bowel bacterial overgrowth and targeting molecules to interrogate or to deliver vaccines to the remote regions of the small intestine has proven difficult. There is an unmet need for methodologies to release probes/drugs to remote regions of the gastrointestinal tract in furthering our understanding of gut health and pathogenesis. In order to address this concern, we need to know how the regional delivery of a surrogate labeled test compound is handled and in turn, if delivered locally as a liquid or powder, the dynamics of its subsequent handling and metabolism. In the studies we report on in this paper, we chose ¹³C sodium acetate (¹³C-acetate), which is a stable isotope probe that once absorbed in the small intestine can be readily measured non-invasively by collection and analysis of ¹³CO₂ in the breath. This would provide information of gastric emptying rates and an indication of the site of release and absorptive capacity. In a series of in vitro and in vivo pig experiments, we assessed the enteric-protective properties of a commercially available polymer EUDRAGIT®L100-55 on gelatin capsules and also on DRcaps®. Test results demonstrated that DRcaps®coated with EUDRAGIT®L100-55 possessed enhanced enteric-protective properties, particularly in vivo. These studies add to the body of knowledge regarding gastric emptying in pigs and also begin the process of gathering specifications for the design of a simple and cost-effective enteric-coated capsule for delivery of acid-labile macromolecules to the small intestine.  相似文献   

Maltreatment and coping strategies among male adolescents living in the Gaza Strip   总被引：2，自引：0，他引：2  

Thabet AA  Tischler V  Vostanis P 《Child abuse & neglect》2004,28(1):77-91

OBJECTIVE: To establish the nature and extent of maltreatment experiences, coping strategies, and behavioral/emotional problems, and their relationships, in a sample of Palestinian adolescents. METHOD: A study of 97 male adolescents aged 15-19 years, and attending a vocational training center based in the Gaza Strip. Adolescents completed the Child Maltreatment Schedule and the Ways of Coping Scale (WAYS). The Strengths and Difficulties Questionnaire (SDQ) was completed by adolescents and by their teachers. RESULTS: Findings revealed high rates of emotional and physical maltreatment. Reliance on emotion-focused or avoidant coping strategies was associated with exposure to maltreatment. Use of maladaptive coping also predicted emotional difficulties in the respondents. CONCLUSIONS: Coping strategies are an important indicator of psychosocial functioning in adolescents who have experienced maltreatment. Identification of coping styles can augment the assessment of at-risk adolescents. Emotion-focused strategies, in particular, appear to be widely used by young people from non-Western cultural backgrounds.  相似文献   

Endophytes from medicinal plants and their potential for producing indole acetic acid,improving seed germination and mitigating oxidative stress     

Abdul Latif Khan  Syed Abdullah Gilani  Muhammad Waqas  Khadija Al-Hosni  Salima Al-Khiziri  Yoon-Ha Kim  Liaqat Ali  Sang-Mo Kang  Sajjad Asaf  Raheem Shahzad  Javid Hussain  In-Jung Lee  Ahmed Al-Harrasi 《Journal of Zhejiang University. Science. B》2017,18(2):125-137

Medicinal plants have been used by marginal communities to treat various ailments. However, the potential of endophytes within these bio-prospective medicinal plants remains unknown. The present study elucidates the endophytic diversity of medicinal plants (Caralluma acutangula, Rhazya stricta, and Moringa peregrina) and the endophyte role in seed growth and oxidative stress. Various organs of medicinal plants yielded ten endophytes, which were identified as Phoma sp. (6 isolates), Alternaria sp. (2), Bipolaris sp. (1), and Cladosporium sp. (1) based on 18S rDNA sequencing and phylogenetic analysis. The culture filtrates (CFs; 25%, 50%, and 100% concentrations) from these endophytes were tested against the growth of normal and dwarf mutant rice lines. Endophytic CF exhibited dose-dependent growth stimulation and suppression effects. CF (100%) of Phoma sp. significantly increased rice seed germination and growth compared to controls and other endophytes. This growth-promoting effect was due to the presence of indole acetic acid in endophytic CF. The gas chromatography/mass spectrometry (GC/MS) analysis showed the highest indole acetic acid content ((54.31±0.21) μmol/L) in Bipolaris sp. In addition, the isolate of Bipolaris sp. exhibited significantly higher radical scavenging and anti-lipid peroxidation activity than the other isolates. Bipolaris sp. and Phoma sp. also exhibited significantly higher flavonoid and phenolic contents. The medicinal plants exhibited the presence of bio-prospective endophytic strains, which could be used for the improvement of crop growth and the mitigation of oxidative stresses.  相似文献   

Assessment of the phase synchronization effect in modal testing during operation     

Zhi Chao Ong  Hong Cheet Lim  Shin Yee Khoo  Zubaidah Ismail  Keen Kuan Kong  Abdul Ghaffar Abdul Rahman 《浙江大学学报(A卷英文版)》2017,18(2):92-105

The impact-synchronous modal analysis (ISMA), which uses impact-synchronous time averaging (ISTA), allows modal testing to be performed during operation. ISTA is effective in filtering out the non-synchronous cyclic load component, its harmonics, and noises. However, it was found that at operating speeds that coincide with the natural modes, ISMA would require a high number of impacts to determine the dynamic characteristics of the system. This finding has subsequently reduced the effectiveness and practicality of ISMA. Preservation of signatures during ISTA depends on the consistency of their phase angles on every time block but not necessarily on their frequencies. Thus, the effect of phase angles with respect to impact is seen to be a very important parameter when performing ISMA on structures with dominant periodic responses due to cyclic load and ambient excitation. The responses from unaccounted forces that contain even the same frequency as that contained in the response due to impact are diminished with the least number of impacts when the phase of the periodic responses is not consistent with the impact signature for every impact applied. The assessment showed that a small number of averages are sufficient to eliminate the non-synchronous components with 98.48% improvement on simulation and 95.22% improvement on experimental modal testing when the phase angles with respect to impact are not consistent for every impact applied.  相似文献   

Book reviews     

Jacques vauclair  João Batista Araujo e Oviveira  Guy Neave  Abdel Aziz el Koussy 《Prospects》1976,6(3):481-494

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Effect of dietary fish on the lipid profile of cholesterolstressed rats during copper overload     

M. Nandini  M. Abdul Rahiman  S. Narasimha Rao 《Indian journal of clinical biochemistry : IJCB》2000,15(2):161-166

The effect of dietary supplementation with Oil sardine (Sardinella longiceps) fish at 2/3 fat replacement, on serum lipids in the cholesterol-stressed copper loaded rats was studied. Hypercholesterolemic state observed during copper toxicity was not alleviated by sardines. Copper loading significantly decreased the triglyceride levels and the activity of β-hydroxy β-methyl glutaryl (HMG) CoA reductase. Fish supplementation was further effective in reducing the triglyceride levels in copper loaded rats. Significant increase in the serum phospholipid was observed in the fish supplemented rats during copper overload. The finding suggests that hypolipidemic potential of fish is certainly altered to some extent in copper toxicity since, it is well known that copper enhances the formation of free radicals. Thus it may be concluded that sardine fish is not effective in reducing the cholesterol levels during copper toxicity.  相似文献   

Interpreting Cues of Goodness: Thin Sliced Judgment of Malaysians and Chinese towards Political Leaders     

Madiha A. Hashmi  Ezhar Tamam  Moniza Waheed  Abdul Muati Ahmad 《Journal of Intercultural Communication Research》2016,45(5):419-436

Thin slice studies have demonstrated that quick personality inferences of unknown others can be made fairly accurately. Utilizing focus group interviews, the current study examines thin sliced judgments and perceptions of six leaders in the context of goodness. Findings revealed that perceivers were able to form relatively accurate impressions of some leaders based on brief glimpses of nonverbal footage. Across all groups (Malaysian and Chinese) paralinguistic cues, dynamic cues and static cues were instrumental in the formation of positive perceptions of leaders in the context of goodness. These findings have implications for the areas of nonverbal behaviour, political communication, and personality judgement.  相似文献   

The religious,the plural,the secular and the modern: a brief critical survey on Islam in Malaysia     

Azmi Aziz  A. B. Shamsul 《Inter-Asia Cultural Studies》2013,14(3):341-356

Abstract

The embedization of Islam in Malaysia has gone through a long and complex process that involved an interaction with three major world civilizations (Indian, Chinese and European) and two colonial systems (Dutch and British) during which many aspects of its practices were reconfigured. This paper provides a brief critical survey of the evolution of the said embedization process during which Islam and the Muslims in Malaysia were moulded by a series of sociological realities, namely plural society, secularism and modernity. This has resulted, we argue, in the creation of 'moderate' Islam in Malaysia, one that is quite different from the fundamentalist image of Islam profiled in the contemporary worldwide discourse on global Islam  相似文献   

Teacher professional experience and performance: impact of the work environment and general welfare in Malaysian secondary schools     

Suseela Malakolunthu  Abdul Rahman Idris  Nagappan C. Rengasamy 《Asia Pacific Education Review》2010,11(4):609-617

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Piper sarmentosum as an antioxidant on oxidative stress in human umbilical vein endothelial cells induced by hydrogen peroxide     

Abdul Hamid HAFIZAH  Zakaria ZAITON  Amom ZULKHAIRI  Adenan MOHD ILHAM  Megat Mohd Nordin NOR ANITA  Abdullah Mahdy ZALEHA 《Journal of Zhejiang University. Science. B》2010,11(5):357-365

Endothelial cell death due to increased reactive oxygen species(ROS) may contribute to the initial endothelial injury,which promotes atherosclerotic lesion formation.Piper sarmentosum(PS),a natural product,has been shown to have an antioxidant property,which is hypothesized to inhibit production of ROS and prevent cell injury.Thus,the present study was designed to determine the effects of PS on the hydrogen peroxide(H2O2)-induced oxidative cell damage in cultured human umbilical vein endothelial cells(HUVECs).In this experiment,HUVECs were obtained by collagenase perfusion of the large vein in the umbilical cord and cultured in medium M200 supplemented with low serum growth supplementation(LSGS).HUVECs were treated with various concentrations of H2O2(0-1000 μmol/L) and it was observed that 180 μmol/L H2O2 reduced cell viability by 50% as denoted by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay.Using the above concentration as the positive control,the H2O2-induced HUVECs were concomitantly treated with various concentrations(100,150,250 and 300 μg/ml) of three different extracts(aqueous,methanol and hexane) of PS.Malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase(GPX) levels showed a significant increase(P0.05) in HUVECs compared to the negative control.However,PS extracts showed a protective effect on HUVECs from H2O2-induced cell apoptosis with a significant reduction in MDA,SOD,CAT and GPX levels(P0.05).Furthermore,PS had exhibited ferric reducing antioxidant power with its high phenolic content.Hence,it was concluded that PS plays a beneficial role in reducing oxidative stress in H2O2-induced HUVECs.  相似文献