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21.
Liquid dielectrophoresis (L-DEP), when deployed at microscopic scales on top of hydrophobic surfaces, offers novel ways of rapid and automated manipulation of very small amounts of polar aqueous samples for microfluidic applications and development of laboratory-on-a-chip devices. In this article we highlight some of the more recent developments and applications of L-DEP in handling and processing of various types of aqueous samples and reagents of biological relevance including emulsions using such microchip based surface microfluidic (SMF) devices. We highlighted the utility of these devices for on-chip bioassays including nucleic acid analysis. Furthermore, the parallel sample processing capabilities of these SMF devices together with suitable on- or off-chip detection capabilities suggest numerous applications and utility in conducting automated multiplexed assays, a capability much sought after in the high throughput diagnostic and screening assays. 相似文献
22.
H. Ravi Kumar K. S. Devaraju B. M. Prakash S. Sampath Kumar S. V. Suresh Babu N. Ramachandraswamy H. P. Puttaraju 《Indian journal of clinical biochemistry : IJCB》2009,24(3):275-279
To establish/develop an assay method for measuring Ornithine Aminotransferase (EC.2.6.1.13) activity using rat brain mitochondria
as a source of enzyme in presence and absence of Pyridoxal Phosphate (PLP). The modified method, with the improved sensitivity,
is adopted for the assay of ornithine amino transferase activity in rat brain mitochondria. The enzyme activity was measured
at 620 nm, the study showed that reaction was optimum at 37°C for 30 minutes. The assay is sensitive enough to detect activity
at the order of nanomoles pyrroline-5-carboxylate/mg protein/minute and can be compared as an alternative to the radio isotopic
method which is more cumbersome and aminobenzaldehyde method which is less sensitive. The Km & Vmax shows maximum activity in the presence of Pyridoxal Phosphate (Coenzyme) concentration at 0.05mM when compared with absence
of Pyridoxal Phosphate as higher the concentration of Pyridoxal Phosphate affects the affinity of the enzyme to substrate.
The OAT activity in different tissues of the rat was also studied and highest activity was found in liver and kidney. 相似文献
23.
Ravi Parkash 《Resonance》1998,3(2):28-34
The increasing power of molecular techniques of gene cloning, DNA sequencing and use of restriction enzymes has revolutionised our ability to understand mechanisms of evolution at the level of genes. Exciting new findings on pseudogenes and gene elongation have led to an increased understanding of how our genes themselves change through evolutionary time, often in bizzarre ways. 相似文献
24.
We present here a set of simple experiments through which various characteristics of a DC motor can be investigated. The experiments also provide insights on how to control a DC motor. We first study the back-emf versus angular speed and the torque versus current characteristics. We then extract the moment of inertia of the rotor and the speedtorque curve for the load from the start-stop dynamics of the motor. Finally, we study an alternate way of obtaining the angular speed from the back-emf waveform. 相似文献
25.
S. Pandey Ravi Ranjan R. M. Mishra Sw. Pandey R. Saxena 《Indian journal of clinical biochemistry : IJCB》2012,27(3):314-317
Hemoglobin D exist in four form; HbD trait, HbD-thalassemia, HbD sickle cell and HbD homozygous. HbD trait and HbD homozygous generally asymptomatic condition but when HbD co-inherit with thalassemia and sickle cell anemia, produces clinically significant conditions like chronic hemolytic anemia. Here we present a case of HbD Punjab with α 3.7 kb deletion and IVS-1-5 β-thalassemia across a family. Diagnosis of HbD patient was performed by high performance liquid chromatography and complete blood count was measured by automated cell analyzer. Molecular study for common alpha deletions done by Gap-PCR while beta thalassemia mutation identified by ARMS-PCR. Case was clinically significant due to the inheritance of HbD/β+thalassemia genotype. Thus observed case behaved like thalassemia intermedia due to co-existence of α 3.7 deletions with IVS 1-5 β-thalassemia mutation in HbD Punjab patient. 相似文献
26.
S. Pandey R. M. Mishra A. Suhail S. Rahul K. Ravi Sw. Pandey T. Seth R. Saxena 《Indian journal of clinical biochemistry : IJCB》2012,27(3):270-273
Usually sickle cell traits are asymptomatic but co-existence of various factrors may alter the clinical as well as biochemical levels. In India sickle cell traits are neglected condition. Here we are presenting the alpha deletion in association with low serum iron and increased HbF level with Xmn-1 carriers in sickle cell traits. Sickle traits with alpha deletions had significantly low level of serum iron (P-value <0.05) with low level of reticulocytes and red cell indices while Xmn-1 polymorphism associated with increased HbF level. Study concludes low serum iron associated with alpha deletions and high level of HbF associated with Xmn-1 polymorphism in sickle cell traits. 相似文献
27.
Anuradha Bharosay Vivek Vikram Bharosay Meena Varma Kiran Saxena Ajoy Sodani Ravi Saxena 《Indian journal of clinical biochemistry : IJCB》2012,27(2):186-190
Stroke is the third major cause of death and foremost cause of disability worldwide. Cerebrovascular stroke remains largely a clinical diagnosis. The use of biomarkers in diagnosing stroke and assessing prognosis is an emerging and rapidly evolving field. The study aimed to investigate the predictive value of neurobiochemical marker of brain damage (neuron-specific enolase [NSE]) with respect to degree of disability at the time of admission and neurological worsening in acute ischemic stroke patients. We investigated 150 patients with cerebrovascular stroke who were admitted within 72 h of onset of stroke in the Department of Neurology at SAIMS. Venous blood samples were taken after admission and NSE was analyzed by solid enzyme linked immunosorbent assay using Analyzer and microplate reader from Biored: Code 680. In all patients, the neurological status was evaluated by a standardized neurological examination and the National Institutes of Health Stroke Scale on admission and on day 7. Serum NSE concentration was found to significantly correlate with both degree of disability and neurological worsening in acute ischemic stroke cases in the present study. The maximum serum NSE level within 72 h of admission was significantly higher in patients with greater degree of disability at the time of admission. Serum NSE levels were also found to be significantly elevated in patients with bad neurological outcome. Our study showed that serum NSE has high predictive value for determining severity and early neurobehavioral outcome after acute stroke. 相似文献
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29.
This paper describes a research study which examined the degree to which the levels of English proficiency (high vs. low) and education (graduate vs. undergraduate) of students for whom English is a second language (ESL) were associated with differences in their reading behavior patterns. Subjects completed a survey which elicited information about several aspects of their reading behavior patterns including the type of materials read, the amount of time devoted to each type of reading, perceptions of their own reading ability, weaknesses, and needed improvements in reading. The results revealed that many of the mean scores obtained differed significantly, a fact indicating that the subjects’ levels of education and English proficiency were indeed associated with their reading behavior patterns. The paper concludes with a discussion of the instructional implications of the findings. 相似文献
30.
K. Ravi Srinivas 《The Information Society》2013,29(3):227-228
Abstract The efforts of other nations to develop comprehensive approaches to the development and application of information technology have given rise to concern in many quarters about the adequacy of the U.S. government's response to information issues. A bill, H.R. 3137, was introduced in the House of Representatives in April 1981 by Congressman George E. Brown, Jr., in an attempt to stimulate movement toward a coordinated development of U.S. information policies. Hearings on this legislation elicited a wide variety of opinion concerning how best to proceed toward this development. This paper surveys the testimony presented at the hearings and some recent actions taken by Congress and by the Reagan Administration on information issues. 相似文献