罗格列酮改善链脲霉素诱导的糖尿病小鼠认知障碍及机制研究     

张亭亭  刘丽萍  姜荔莹  胡伟  龙燕  洪浩 《河北北方学院学报(医学版)》2011,1(2)

目的:研究罗格列酮(rosiglitazone)对链脲霉素诱导的1 型糖尿病小鼠认知功能的影响及其作用机制.方法:单剂量尾静脉注射(iv)链脲霉素(150 mg·kg-1)诱导小鼠1 型糖尿病模型,将造模成功(空腹血糖>11.1 mmol·L-1)的小鼠随机分为3 组:模型组,罗格列酮3.2,1.6 mg·kg-1·d-1 剂量组;另设正常对照组,每组10～12只,连续灌胃(ig)给药6 周.第6 周采用Morris 水迷宫和Y 迷宫实验评价认知功能,并检测空腹血糖、血清胰岛素、海马和皮层区Aβ40,Aβ42,糖尿病动物脑内β分泌酶(β-site amyloid precursor protein cleaving enzyme,BACE1)含量.结果:与模型组相比,罗格列酮3.2 mg·kg-1 剂量组能显著缩短糖尿病小鼠定位航行试验的潜伏期(P<0.05),增加空间探索实验中平台所在象限的停留时间百分率(P<0.05),并增加糖尿病小鼠在Y 迷宫测试中的正确反应次数(P<0.05);罗格列酮对1 型糖尿病小鼠空腹血糖和胰岛素水平无显著影响;罗格列酮3.2 mg·kg-1 剂量显著降低糖尿病小鼠脑内海马和皮层区Aβ40(P<0.05,P<0.05)和Aβ42(P<0.05,P<0.05)的水平而对海马和皮层区BACE1 水平无显著影响.结论:罗格列酮3.2 mg·kg-1 剂量可通过降低脑内Aβ水平而改善1 型糖尿病小鼠认知障碍.  相似文献   

结肠腺瘤性息肉病蛋白在大脑皮层早期发育中的作用     

左玮  季晖  王文 《河北北方学院学报(医学版)》2011,1(2)

结肠腺瘤性息肉病(adenomatous polyosis coli,APC)蛋白是一个大型的多功能蛋白,在发育的大脑皮层中,增殖的细胞可以表达APC,且当细胞向皮层板迁移的时候它的表达会增加.其对于Wnt/β-catenin 信号,细胞骨架动态以及细胞极性十分重要.本文对APC 在大脑皮层早期发育中的作用进行了总结.APC 在大脑皮层早期发育过程中是调节细胞数目,核迁移运动,细胞极性以及细胞类型的特化所必须的.  相似文献   

远志总皂苷对AD模型大鼠海马nAChRα4及PSD-95表达的影响     

李晓峰  赵大鹏  陈树沙  景玮  李新毅 《河北北方学院学报(医学版)》2011,1(3):16-22

目的:通过观察中药远志总皂苷(Tenuigenin,TEN)对阿尔茨海默病(Alzheimer’s disease,AD)模型大鼠烟碱型乙酰胆碱受体α4亚基(nicotinic acetylcholine receptor subunit alpha-4,nAChRα4)和突触后致密蛋白95(postsynaptic density 95,PSD-95)表达的影响,旨在探讨TEN对AD干预作用的机制。方法:32只雄性Wistar大鼠随机分为对照组,模型组,TEN 12.5,37.5 mg.mL-1剂量组,应用D-半乳糖致衰老的基础上定向Meynert基底核损毁建立AD大鼠模型,TEN 12.5,37.5 mg.mL-1治疗组则在造模的同时用远志总皂苷灌胃(ig)治疗,采用免疫组化方法来检测大鼠海马CA1区nAChRα4及PSD-95的表达。结果:模型组大鼠海马CA1区nAChRα4及PSD-95平均灰度值显著高于对照组,平均光密度值显著降低(P<0.01);TEN 12.5,37.5 mg.mL-1剂量组大鼠海马nAChRα4及PSD-95平均灰度值显著低于模型组,两组平均光密度值均显著增高(P<0.05);...  相似文献   

Evaluation of the antioxidant effects of four main theaflavin derivatives through chemiluminescence and DNA damage analyses   总被引：1，自引：0，他引：1  

Wu YY  Li W  Xu Y  Jin EH  Tu YY 《Journal of Zhejiang University. Science. B》2011,12(9):744-751

Theaflavins (TFs) are the dimers of a couple of epimerized catechins, which are specially formed during black tea fermentation. To explore the differences among four main TF derivatives (theaflavin (TF₁), theaflavin-3-gallate (TF₂A), theaflavin-3′-gallate (TF₂B), and theaflavin-3,3′-digallate (TF₃)) in scavenging reactive oxygen species (ROS) in vitro, their properties of inhibiting superoxide, singlet oxygen, hydrogen peroxide, and the hydroxyl radical, and their effects on hydroxyl radical-induced DNA oxidative damage were systematically analyzed in the present study. The results show that, compared with (−)-epigallocatechin gallate (EGCG), TF derivatives were good antioxidants for scavenging ROS and preventing the hydroxyl radical-induced DNA damage in vitro. TF₃ was the most positive in scavenging hydrogen peroxide and hydroxyl radical, and TF₁ suppressed superoxide. Positive antioxidant capacities of TF₂B on singlet oxygen, hydrogen peroxide, hydroxyl radical, and the hydroxyl radical-induced DNA damage in vitro were found. The differences between the antioxidant capacities of four main TF derivatives in relation to their chemical structures were also discussed. We suggest that these activity differences among TF derivatives would be beneficial to scavenge different ROS with therapeutic potential.  相似文献   

Characterization of (68)Zn uptake, translocation, and accumulation into developing grains and young leaves of high Zn-density rice genotype     

Wu CY  Feng Y  Shohag MJ  Lu LL  Wei YY  Gao C  Yang XE 《Journal of Zhejiang University. Science. B》2011,12(5):408-418

Zinc (Zn) is an essential micronutrient for humans, but Zn deficiency has become serious as equally as iron (Fe) and vitamin A deficiencies nowadays. Selection and breeding of high Zn-density crops is a suitable, cost-effective, and sustainable way to improve human health. However, the mechanism of high Zn density in rice grain is not fully understood, especially how Zn transports from soil to grains. Hydroponics experiments were carried out to compare Zn uptake and distribution in two different Zn-density rice genotypes using stable isotope technique. At seedling stage, IR68144 showed higher ⁶⁸Zn uptake and transport rate to the shoot for the short-term, but no significant difference was observed in both genotypes for the long-term. Zn in xylem sap of IR68144 was consistently higher, and IR68144 exhibited higher Zn absorption ratio than IR64 at sufficient (2.0 μmol/L) or surplus (8.0 μmol/L) Zn supply level. IR64 and IR68144 showed similar patterns of ⁶⁸Zn accumulation in new leaves at seedling stage and in developing grains at ripening stage, whereas ⁶⁸Zn in new leaves and grains of IR68144 was consistently higher. These results suggested that a rapid root-to-shoot translocation and enhanced xylem loading capacity may be the crucial processes for high Zn density in rice grains.  相似文献   

甲磺酸双氢麦角毒碱片含量均匀度测定方法学研究     

牟微  常明  任蕾  王婧  熊婷  余云霓 《石家庄学院学报》2011,13(3):33-35

建立甲磺酸双氢麦角毒碱片的含量均匀度测定方法.采用对二甲氨基苯甲醛作为显色剂与甲磺酸双氢麦角毒碱显色,以580 nm为测定波长,采用紫外分光光度法进行测定.本方法测定的线性范围47.79～77.66 mg/L(r=0.999 9),平均回收率(n=9)为100.9%.本法简便、快速、准确,可较好评价甲磺酸双氢麦角毒碱片的含量均匀度,为其质量控制提供依据.  相似文献   

依托度酸双层渗透泵控释片的制备     

常明  岳红坤  牟微  李超  刘树彬 《石家庄学院学报》2011,13(3):14-17,78

目的:制备依托度酸双层渗透泵控释片.方法:采用单因素法考察依托度酸渗透泵控释片体外释药行为的影响.结果:最佳片剂处方组成为:包衣膜增重为15%,聚氧乙烯(PEO)65%,CMS-Na 56 mg,HPMCK100M 45 mg,CP971NF 11.6 mg,PVPK30 22 mg,NaCl 32.5 mg.控释片批间重复性良好.结论:自制依托度酸双层渗透泵控释片工艺稳定,零级释药特征明显,24 h内释药完全.  相似文献   

项目导向、案例教学在VB程序设计教学中的运用   总被引：1，自引：0，他引：1  

项伟 《长江工程职业技术学院学报》2011,28(1):75-76

本文结合项目导向、案例教学的优点,根据VB课程的内容及特点,把项目导向、案例教学运用于VB课程的教学中进行研究与探索,取得了良好的教学效果。  相似文献   

A nonlinear dynamic macro-element for demand assessment of bridge substructures subjected to ship collision     

Wei Fan  Wan-cheng Yuan  Mi Zhou 《浙江大学学报(A卷英文版)》2011,12(11):826-836

For the dynamic demand assessment of bridge structures under ship impact loading, it may be prudent to adopt analytical models which permit rapid analysis with reasonable accuracy. Herein, a nonlinear dynamic macro-element is proposed and implemented to quantify the demand of bridge substructures subjected to ship collisions. In the proposed nonlinear macro-element, a combination of an elastic-plastic spring and a dashpot in parallel is employed to describe the mechanical behavior of ship-bows with strain rate effects. Based on the analytical model using the proposed macro-element, a typical substructure under 5000 deadweight tonnage (DWT) ship collision is discussed. Our analyses indicate that the responses of the structure using the nonlinear macro-element agree with the results from the high resolution model, but the efficiency and feasibility of the proposed method increase significantly in practical applications. Furthermore, comparisons between some current design codes (AASHTO, JTGD60-2004, and TB10002.1-2005) and the developed dynamic analysis method suggest that these design codes may be improved, at least to consider the effect of dynamic amplification on structural demand.  相似文献   

Optimal linear attitude estimators via geometric analysis     

De-ren Gong  Xiao-wei Shao  Wei Li  Deng-ping Duan 《浙江大学学报(A卷英文版)》2011,12(11):873-882

Three optimal linear attitude estimators are proposed for single-point real-time estimation of spacecraft attitude using a geometric approach. The final optimal attitude is represented by modified Rodrigues parameters (MRPs). After introducing incidental right-hand orthogonal coordinates for each pair of measured values, three error vectors are obtained by the use of dot or/and cross products. Corresponding optimality criteria are rigorously quadratic and unconstrained, which do not coincide with Wahba’s constrained criterion. The singularity, which occurs when the principal angle is close to π, can be easily avoided by one proper rotation. Numerical simulations show that the proposed three optimal linear estimators can provide a precision comparable with those complying with the Wahba optimality definition, and have faster computational speed than the famous quaternion estimator (QUEST).  相似文献