Differential pH sensitivity of tissue superoxide dismutases     

Samir P Patel  Surendra S Katyare 《Indian journal of clinical biochemistry : IJCB》2006,21(2):129-133

Superoxide dismutase (SOD) activities in the human and rat RBCs and rat liver, kidney, brain and heart mitochondria as well as cytosolic fractions were determined by the pyrogallol assay procedure with slight modifications. Measurements were carried out in 0.1 M potassium phosphate buffer pH 8.0 and 9.2 to assess the pH stability of the SODs from various systems. Under these conditions the SODs from different systems including RBCs exhibited differential pH stability i.e. they displayed differential susceptibility at pH 9.2. Even in a given tissue, the mitochondrial and cytosolic SODs contents show a tissue-specific pattern. Our results also suggest that measurements carried out at pH 8.0 may give more realistic estimates of SOD activities.  相似文献   

基于GIS的中国酸雨控制区酸雨空间分布特征研究   总被引：1，自引：0，他引：1  

徐晓华  徐光来 《石家庄学院学报》2014,(3):77-83

利用全国74个酸雨监测站点2004-2006年降水-pH数据,通过ArcGIS空间插值,模拟酸雨pH空间分布,根据酸雨"两控区"方案,提取酸雨控制区的酸雨pH空间分布,进行GIS空间分析.结果表明:中国的酸雨严重区主要分布在四川盆地、长江以南广大地区,酸雨强度沿长江向西北和东部有扩大趋势,四川盆地酸雨区已与华南酸雨区连成一片;3年间,pH<5.6的酸雨范围相对研究区面积比值由56.73%扩大到73.11%,pH<4.5的严重酸雨区由2.15%扩大到10.41%,2006年增幅最大;不同等级酸雨范围扩大趋势明显,酸雨污染趋于严重.  相似文献   

pH值及热处理对黄连素抑菌活性的影响     

王信  罗琴  杨雪梅  谭军  陈菊梅  茶映妍  鲁青 《楚雄师范学院学报》2014,29(6):53-57

用滤纸片法测定黄连素的抑菌活性。结果显示黄连素有较宽的抗菌谱,黄连素对以下五种细菌:革兰氏阴性菌大肠杆菌及革兰氏阳性菌枯草芽孢杆菌、金黄色葡萄球菌的抑制作用较强,对真菌黑曲霉菌的抑制作用稍弱;黄连素抑菌活性不受热处理的影响,而受pH值影响较大,在pH值为4—8范围内,黄连素抑菌活性较好且稳定。采用液体稀释法,研究了黄连素的最小抑菌浓度(MIC)。结果表明,黄连素对金黄色葡萄球菌的最小抑菌浓度为62.5mg/L,对枯草芽孢杆菌的最小抑菌浓度为31.5mg/L,对大肠杆菌的最小抑菌浓度为125mg/L,对黑曲霉的最小抑菌浓度为500mg/L。  相似文献