果蝇EF-1α蛋白的原核表达及多克隆抗体制备     

戴悦  任恋  彭佩莹  王盼盼  袁婺洲  吴秀山 《怀化师专学报》2013,(5):33-36

为进一步研究延长因子-1（elongationfactor-1α,E-1α）在调控心脏发育和果蝇心脏功能中发挥的作用,制备延长因子-1的抗体．利用生物信息学选择果蝇EF-1α基因抗原亲水区,并设计出特异性引物,PCR扩增出的片段克隆到原核表达pET-28a载体中,转入E．coli中后通过IPTG诱导融合蛋白表达,将His—EF-1α融合蛋白免疫新西兰大白兔制备多克隆抗体,再用WesternBlot检测抗体的效价和特异性．研究结果表明,获得了EF-1α原核表达重组融合蛋白以及高效价的、特异性兔抗EF-1α多克隆抗体．EF-1α多克隆抗体的效价可以达到1比1500,并且有很强的特异性,为后续研究EF-1α基因在心脏发育和心脏功能中的作用奠定了基础．  相似文献   

大理州2010年至2012年凝难配血结果分析     

何永勋  王华  杜月娥  刁丽波 《大理师专学报》2013,(9):68-70

目的：全面了解大理州近3年来各医院送检的凝难配血情况。方法：对大理州中心血站2010年至2012年血型参比室处理的凝难配血报告结果作分析。结果：显示送检的26份血样中18份存不规则抗体;而且Rh血型系统不规则抗体检出的比例最高（27．7％）;其余次之。8份为医院检验科或输血科聚凝氨试验法或卡式抗人球蛋白法出现凝集;经本室抗人球蛋白等一系列试验检测;未发现异常。结论：受检测患者中Rh血型系统不规则抗体检出的比例最高（27．7％）;其余次之。此外,医院配血时忽略了抗人球蛋白试验的操作细节,会将非特异性的凝集误判为特异性抗体引起的凝集。  相似文献   

Advances in monoclonal antibody application in myocarditis     

Li-na Han  Shuang He  Yu-tang Wang  Li-ming Yang  Si-yu Liu  Ting Zhang 《Journal of Zhejiang University. Science. B》2013,14(8):676-687

Monoclonal antibodies have become a part of daily preparation technologies in many laboratories. Attempts have been made to apply monoclonal antibodies to open a new train of thought for clinical treatments of autoimmune diseases, inflammatory diseases, cancer, and other immune-associated diseases. This paper is a prospective review to anticipate that monoclonal antibody application in the treatment of myocarditis, an inflammatory disease of the heart, could be a novel approach in the future. In order to better understand the current state of the art in monoclonal antibody techniques and advance applications in myocarditis, we, through a significant amount of literature research both domestic and abroad, developed a systematic elaboration of monoclonal antibodies, pathogenesis of myocarditis, and application of monoclonal antibodies in myocarditis. This paper presents review of the literature of some therapeutic aspects of monoclonal antibodies in myocarditis and dilated cardiomyopathy to demonstrate the advance of monoclonal antibody application in myocarditis and a strong anticipation that monoclonal antibody application may supply an effective therapeutic approach to relieve the severity of myocarditis in the future. Under conventional therapy, myocarditis is typically associated with congestive heart failure as a progressive outcome, indicating the need for alternative therapeutic strategies to improve long-term results. Reviewing some therapeutic aspects of monoclonal antibodies in myocarditis, we recently found that monoclonal antibodies with high purity and strong specificity can accurately act on target and achieve definite progress in the treatment of viral myocarditis in rat model and may meet the need above. However, several issues remain. The technology on how to make a higher homologous and weak immunogenic humanized or human source antibody and the treatment mechanism of monoclonal antibodies may provide solutions for these open issues. If we are to further stimulate progress in the area of clinical decision support, we must continue to develop and refine our understanding and use of monoclonal antibodies in myocarditis.  相似文献   

单克隆抗体研究进展——不同表达系统介绍与比较     

张晓筱 《贵州教育学院学报》2007,18(4):56-61

单克隆抗体的高度特异性使其在包括细胞生物学、基础医学、临床诊断等领域得到广泛应用,而各种表达系统的开发进一步促进了单克隆抗体及其衍生物的发展.对目前用于生产单克隆抗体的主要表达系统进行综述,同时比较各系统的特点和优缺点.  相似文献   

连接素抗体与重症肌无力     

朱惠民  张旭 《金华职业技术学院学报》2005,5(4):101-103,109

部分重症肌无力（MG）患者血清中不但有乙酰胆碱受体抗体（AChR Ab）,还存在连接素抗体（Titin Ab）.研究发现约95%的MGT及近50%的晚发MG患者血清中存在Titin Ab;Titin Ab还可以预测胸腺切除术后MG患者的预后.  相似文献   

双抗体ELISA检测鸡传染性法氏囊病病原     

王成明  蔡宝祥  陈溥言 《安徽科技学院学报》1995,(3)

用单克隆抗体双抗体ＥＬＩＳＡ法检测自然发病鸡和人工感染鸡的法氏囊及其它脏器和ＩＢＤＶ细胞毒，病鸡的法氏囊ＩＢＤＶ检出率约１００％，其它脏器较低，ＩＢＤＶ细胞毒毒公明显低于组织毒。  相似文献   

B细胞产生抗体的年龄相关性变化     

吴翰桂  徐素红 《台州学院学报》1997,(3)

衰老动物的B细胞无论是对胸腺依赖性抗原,还是对非胸腺依赖性抗原的免疫应答能力都下降。这不仅表现在抗体的数量上,而且还表现在抗体的质量(对抗原的亲和力)上。  相似文献   

ELISA法检测胰岛素依赖型糖尿病患者血清中64KD抗体最佳条件的选定     

王鸿  赵彩红  王钦富 《大连大学学报》1997,(4)

本文报告了用自己纯化的BCG65KD热休克蛋白(HSP65)作抗原建立ELISA法检测胰岛素依赖型糖尿病(IDDM)患者血清中抗胰岛细胞64KD蛋白抗体(简称64KD抗体)并对其最适检测条件进行了选定,结果表明,最适条件:BCGHSP65抗原包被浓度为10μg/mL,1:100血清37℃温育2h,辣根过氧化物酶标记的羊抗人IgG(SAH-IgG-HRP)稀释度为1:800,2％BSA-PBS-T作稀释液。  相似文献   

淋巴细胞亚群的功能及其辐射效应   总被引：1，自引：0，他引：1  

苏燎原  徐映东 《科技通报》1993,9(5):342-346

应用淋巴细胞单克隆抗体(McAb)的Panning法或亲和层析分纯 CD_4~+、CD_8~+、B细胞和非CD_4~+细胞,也应用PHA、ConA、PWM和LPS分别激活淋巴细胞,然后用~3H-TdR按入、集落形成及羟基磷灰石方法,探讨了各亚群淋巴细胞的调节功能及其辐射效应.实验结果表明PWM能激活CD_4~+、非CD_4~+和B细胞,PWM细胞能诱导LPS细胞转化,其中一种细胞受照即失去协同作用,PWM细胞受照影响更严重.ConA能激活CD_4~+和CD_8~+细胞,两者均可抑制LPS细胞转化,并能互相增强抑制效应,受照后消失.辐射敏感性比较:ConA细胞和PHA细胞大于PWM细胞,PWM细胞大于LPS细胞,CD_8~+细胞和B细胞大于CD_4~+细胞。  相似文献   

Spectrum of monoclonal gammapathies in Andhra Pradesh     

T. Malati  B. Yadagiri  D. Murali Mohan Krishna  V. Shantaram  D. Raghunadharao  K. Subbarao 《Indian journal of clinical biochemistry : IJCB》2001,16(1):52-59

In the present study, monoclonal gammapathy was identified in a total of 245 patients of plasma cell dyscrasias during period of 1987 to 2000. The monoclonal band was identified in serum by agar gel electrophoresis in all the cases and in urine in a few cases. Characterization of paraprotein (monoclonal immunoglobulin class and light chain type) was carried out by employing immunoelectrophoresis and/or immunofixation electrophoresis using heavy chain specific gamma, alpha, mu, delta and epsilon and light chain specific kappa (K), lambda (λ) antisera. Serum immunoglobulins Ig G, Ig A, and Ig M were estimated by immunoturbidometry. Serum urea, creatinine, uric acid, alkaline phosphatase, total proteins, albumin, calcium and phosphorus were estimated by using routine biochemical methods. Among the 245 cases, 73.1% monoclonal gammapathies were of secretory type and 7.3% were non-secretory. Monoclonal gammapathies were associated with 80.4% of multiple myeloma, 8.9% of solitary plasmacytoma, 4.1% of extra-medullary plasmacytoma, 3.3% of lymphoma and 2.9% of plasma cell leukemia. Classification of secretory monoclonal immunoglobulin revealed monoclonal immunoglobulin Ig G in 74%, Ig A 15% and Ig M in 2.9% cases.  相似文献