Use of antigen prepared from in vitro culture ofPlasmodium falciparum for the detection of antibodies in sera from patients exposed toPlasmodium vivax     

A. A. Mahdi  S. Ahmad  R. K. Singh  K. Jawad 《Indian journal of clinical biochemistry : IJCB》1994,9(1):35-36

The blood stage parasites fromin vitro cultures ofPlasmodium falciparum were employed for the detection of malaria antibodies in patient sera havingP. vivax infections employing indirect fluorescent antibody (IFA) test. The antibody titres obtained for theP. vivax infected sera ranged from 1∶128 to 1∶1024. Control slides smeared with non-immune sera did not show any fluorescence. On the basis of the results of the present study it maybe concluded that antigen prepared fromin vitro cultures ofP. falciparum cross-reacts withP. vivax antibodies.  相似文献   

Spectrum of monoclonal gammapathies in Andhra Pradesh     

T. Malati  B. Yadagiri  D. Murali Mohan Krishna  V. Shantaram  D. Raghunadharao  K. Subbarao 《Indian journal of clinical biochemistry : IJCB》2001,16(1):52-59

In the present study, monoclonal gammapathy was identified in a total of 245 patients of plasma cell dyscrasias during period of 1987 to 2000. The monoclonal band was identified in serum by agar gel electrophoresis in all the cases and in urine in a few cases. Characterization of paraprotein (monoclonal immunoglobulin class and light chain type) was carried out by employing immunoelectrophoresis and/or immunofixation electrophoresis using heavy chain specific gamma, alpha, mu, delta and epsilon and light chain specific kappa (K), lambda (λ) antisera. Serum immunoglobulins Ig G, Ig A, and Ig M were estimated by immunoturbidometry. Serum urea, creatinine, uric acid, alkaline phosphatase, total proteins, albumin, calcium and phosphorus were estimated by using routine biochemical methods. Among the 245 cases, 73.1% monoclonal gammapathies were of secretory type and 7.3% were non-secretory. Monoclonal gammapathies were associated with 80.4% of multiple myeloma, 8.9% of solitary plasmacytoma, 4.1% of extra-medullary plasmacytoma, 3.3% of lymphoma and 2.9% of plasma cell leukemia. Classification of secretory monoclonal immunoglobulin revealed monoclonal immunoglobulin Ig G in 74%, Ig A 15% and Ig M in 2.9% cases.  相似文献   

杀鲑气单胞菌单克隆抗体的制备及其特性分析   总被引：1，自引：0，他引：1  

林娟娟  陈强  刘荭  杨金先 《莆田学院学报》2009,16(2)

应用杂交瘤单克隆抗体技术制备了6株分泌抗杀鲑气单胞菌杀鲑亚种的单抗细胞株,并对其特性进行分析。结果显示:6株单抗中IgM有3株,IgG1有2株,IgG2a有1株,且抗体效价为1:12800—1:51200,检测灵敏度为1.0×105—1.0×108cfu·mL-1。进一步实验证实这些单抗与其他病原菌都无交叉反应。但单抗5C7、7H6与杀日本鲑亚种有交叉反应;单抗8A2与无色亚种存在阳性反应。表明杀鲑气单胞菌亚种之间既有独特的抗原决定簇,又有共同抗原位点。制备的单抗可用于杀鲑气单胞菌的快速诊断和亚种鉴定,为该菌的进一步研究提供必要手段。  相似文献   

Double lock of a potent human therapeutic monoclonal antibody against SARS-CoV-2     

Ling Zhu  Yong-Qiang Deng  Rong-Rong Zhang  Zhen Cui  Chun-Yun Sun  Chang-Fa Fan  Xiaorui Xing  Weijin Huang  Qi Chen  Na-Na Zhang  Qing Ye  Tian-Shu Cao  Nan Wang  Lei Wang  Lei Cao  Huiyu Wang  Desheng Kong  Juan Ma  Chunxia Luo  Yanjing Zhang  Jianhui Nie  Yao Sun  Zhe Lv  Neil Shaw  Qianqian Li  Xiao-Feng Li  Junjie Hu  Liangzhi Xie  Zihe Rao  Youchun Wang  Xiangxi Wang  Cheng-Feng Qin 《国家科学评论(英文版)》2021,8(3):24-36

Receptor recognition and subsequent membrane fusion are essential for the establishment of successful infection by SARS-CoV-2. Halting these steps can cure COVID-19. Here we have identified and characterized a potent human monoclonal antibody, HB27, that blocks SARS-CoV-2 attachment to its cellular receptor at sub-nM concentrations. Remarkably, HB27 can also prevent SARS-CoV-2 membrane fusion. Consequently, a single dose of HB27 conferred effective protection against SARS-CoV-2 in two established mouse models. Rhesus macaques showed no obvious adverse events when administrated with 10 times the effective dose of HB27. Cryo-EM studies on complex of SARS-CoV-2 trimeric S with HB27 Fab reveal that three Fab fragments work synergistically to occlude SARS-CoV-2 from binding to the ACE2 receptor. Binding of the antibody also restrains any further conformational changes of the receptor binding domain, possibly interfering with progression from the prefusion to the postfusion stage. These results suggest that HB27 is a promising candidate for immuno-therapies against COVID-19.  相似文献   

日本血吸虫压电免疫传感器的研究     

何春萍  吴朝阳  沈国励 《怀化学院学报》1999,(2)

在压电石英晶体金电极表面形成MPA（疏基丙酸）单分子膜,经NHS和EDC活化后固定血吸虫抗原（SjAg）,研制成血吸虫抗体（SjAb）压电免疫传感器．研究了包被抗原浓度、抗原抗体结合时间,TSM声波免疫传感的响应特性、重现性等实验条件的影响．压电晶体的频移值与IRS浓度在0－36μg／ml范围内有良好的线性关系．将此传感器用于兔抗血吸虫抗体血清样品的测定,结果令人满意．  相似文献   

日本血吸虫免疫反应中抗原价数及平衡常数Kass的测定     

何春萍  吴朝阳  沈国励 《怀化学院学报》1998,(5)

在压电石英晶体金电极表面形成流基丙酸（MPA）单分子膜,经NHS和EDC活化后固定血吸虫抗原（SjAg）,研制成血吸虫抗体（SjAb）压电免疫传感器．研究测定了抗原价数及免疫反应平衡常数Kass,结果令人满意．  相似文献   

Production and Purification of Rabbit’s Polyclonal Antibody Against Factor VIII     

Simin Sohrabi  Azim Akbarzadeh  Dariush Norouzian  Ali Farhangi  Mehri Mortazavi  Mohammad Reza Mehrabi  Mohsen Chiani  Zahra Saffari  Soheil Ghassemi 《Indian journal of clinical biochemistry : IJCB》2011,26(4):354-359

The attempt is made to produce recombinant factor VIII but the first step in producing such product is production and purification of rabbit’s polyclonal antibody against factor VIII. The second and third steps involve monoclonal antibody and recombinant factor VIII production. Factor VIII is one of the most important coagulating factor where its deficiency leads to diseases like hemophilia type A or classic. It is an inherited disease. Previously, it was obtained through fractionation of blood plasma of blood donors. After processing, factor VIII could be used to manage such patients. Due to transfer of viral disease like hepatitis and HIV through factor VIII obtained by fractionation, high cost of production, insufficiency of the donors and the process of virus removal, thus production of factor VIII through recombinant technology can be useful and helpful. The reaction between antibody and antigen is one the most specific reaction; therefore, such reaction can be employed to identify factor VIII. Thereby, rabbits were injected several times with adjuvant-linked antigen to produce antibody. The antibody was separated from the blood sample, purified and used to identify factor VIII in the research.  相似文献   

不同免疫功能对有氧运动的反应   总被引：7，自引：0，他引：7  

谢红  郝选明 《天津体育学院学报》2006,21(3):246-248

通过观察有氧训练过程中SI（T淋巴细胞刺激指数）及血清中IgA、IgG、IgM抗体的应答与适应过程，比较细胞免疫和体液免疫对有氧运动的不同反应，揭示有氧运动下人体免疫机能的变化规律及可能机理。以16名非体育专业的普通大学女生为受试者，进行8周有氧训练。结果显示：（1）作为对有氧运动的应答，细胞免疫功能出现明显应答反应，且运动后1h应答恢复速度加快，至第8周基本完全恢复；而体液免疫对有氧运动的应答反应不明显。（2）作为对有氧运动的适应，体液免疫功能适应明显滞后于细胞免疫功能。（3）总体来看，人体不同免疫功能在训练中都有为期4周的免疫低下期，随后逐渐调整恢复．这与神经-免疫-内分泌网络相互调节、相互作用有关。  相似文献   

单克隆抗体在肿瘤治疗中的应用     

谷欣  陈国胜  张想竹 《安阳师范学院学报》2007,(5):71-74

随着一些单克隆抗体获准被应用于临床,单抗在肿瘤治疗中的运用得到了快速发展。其中代表性的有抗血管内皮生长因子的单抗bevacizumab(Avastin),和抗表皮生长因子的cetuximab(Erbitux)。结合常规的化疗,bevacizumab能够显著延长结肠癌、乳腺癌及肺癌患者的生命。Cetuximab无论与化疗联合使用或单独使用对于对化疗有耐药性的结肠癌直肠癌均有显著的临床意义。  相似文献   

Immunodiagnosis of tuberculosis: An update     

A. S. Bhatia  Satish Kumar  B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2003,18(2):1-5

Tuberculosis is still a major health problem in most developing countries and its incidence is rising in many developed countries. This resurgence has been attributed to the HIV epidemic and TB has been declared as a global health emergency by WHO in 1993. The diagnosis of tuberculosis mainly depends upon initial clinical suspicion and radiographic findings with subsequent bacteriological confirmation by sputum smear examination and culture. Lack of sensitivity in smear examination, non specificity of radiological findings, extended tum around time ofMycobacterium tuberculosis culture and difficulties in diagnosing paucibacillary, childhood and extrapulmonary tuberculosis has necessitated to explore the utility of immunodiagnosis of tuberculosis as a convenient and cost effective test to supplement clinical information for definite diagnosis. Many commercial tests are available in the market for diagnosis of TB. Most of these tests are based on the detection of IgG, IgA and IgM antibodies to specific mycobacterial antigen or mixture of antigens. Indigenous immunoassay systems have explored excretory-secretory ES-31 mycobacterial antigen for immunodiagnosis of TB. Many a time there is lack of consistent elevation in all the three Ig classes in active infection thus making it more important to determine the ideal antibody isotype assay for reliable diagnosis of tuberculosis and to save the costs of the patient for unnecessary investigations.  相似文献