Use of antigen prepared from in vitro culture ofPlasmodium falciparum for the detection of antibodies in sera from patients exposed toPlasmodium vivax     

A. A. Mahdi  S. Ahmad  R. K. Singh  K. Jawad 《Indian journal of clinical biochemistry : IJCB》1994,9(1):35-36

The blood stage parasites fromin vitro cultures ofPlasmodium falciparum were employed for the detection of malaria antibodies in patient sera havingP. vivax infections employing indirect fluorescent antibody (IFA) test. The antibody titres obtained for theP. vivax infected sera ranged from 1∶128 to 1∶1024. Control slides smeared with non-immune sera did not show any fluorescence. On the basis of the results of the present study it maybe concluded that antigen prepared fromin vitro cultures ofP. falciparum cross-reacts withP. vivax antibodies.  相似文献   

Evaluation of sevafilachek immunoassays and rapid ICT-filariasis test for detection of bancroftian filariasis     

R. Alli  S. Kulkarni  M. V. R. Reddy  B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2001,16(2):207-210

A comparative analysis was made on the utility of SEVAFILACHEK-stick based immunoassays and commercially available ICT-filariasis test to detect active infection in different groups of bancroftian filariasis. The SEVAFILACHEK immunoassays were found to be useful to detect filarial infection in microfilaraemia and in a significant number of clinical filarial cases with acute, chronic and occult clinical manifestations. In the clinical cases, microfilariae are not usually detected in peripheral circulation. Employing SEVAFILACHEK assays 6 and 5 of the 7 samples of patients with chronic filarial disease, and 6 and 5 of 6 microfilaraemic cases gave positivity for filarial IgG antibodies and antigen respectively. Four of the 6 occult filarial samples were positive for antibodies and antigen. Filarial antigen was detected by ICT-filariasis test in blood samples of all the 6 microfilariaemic cases, 1 chronic filarial and 2 occult filarial samples. The main advantage of ICT assay is its rapid format and convenience for field use.  相似文献   

Immunomodulation of ovalbumin-specific IgG and other classes of antibody response by honey in mice     

Govinda Rao Duddukuri  Y. Vasudeva Rao  D. N. Rao  Rao R Athota 《Indian journal of clinical biochemistry : IJCB》2001,16(1):89-94

It was reported earlier that intraperitoneal administration of honey had immunosuppressive activity on elicitation of allergen-specific murine antibody response as evaluated by passive cutaneous anaphylaxis and double immunodiffusion methods. In this study, the immunomudulatory effect of honey is evaluated by enzyme linked immunosorbent assay (ELISA) using ovalbumin as model allergen. It was found that ovalbumin (OVA)-specific IgG antibody responses elicited with various doses of OVA were significantly suppressed by rock bee honey (p<0.01). Honey was also found to have inhibited the production of OVA-specific IgM, IgA, IgG₁, and IgG_2b whereas that of IgG_2a and IgG₃ were not affected. Furthermore, honey also suppressed the OVA-specific total IgG antibody response in various inbred mice with different genetic background. In addition, the suppressive activity of honey was examined in different groups of mice by injecting honey at different time intervals, before and after immunization with OVA. The anti-OVA IgG antibody response was suppressed significantly when honey was injected 12 hours prior/latter to OVA injection. These results confirm the suppressive activity of honey on antibody response and suggest possible clinical application.  相似文献   

Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine   总被引：2，自引：0，他引：2  

Jian-Xiang Wu  Shao-en Zhang  Xue-ping Zhou 《Journal of Zhejiang University. Science. B》2010,11(1):52-60

A protein conjugate of streptomycin (streptomycin-bovine serum albumin (BSA) conjugate) was prepared and used as immunogen to produce monoclonal antibodies (MAb). One hybridoma secreting anti-streptomycin MAb was obtained and then used to produce MAb. The MAb named 13H5 showed the 50% maximal inhibitory concentration (IC50) value of 4.65 ng/ml and the IC20 value of 0.21 ng/ml in phosphate buffered saline (PBS). At optimum conditions, an indirect competitive enzyme-linked immunosorbent assay (ELISA) and a co...  相似文献   

Nicotinamide <Emphasis Type="Italic">N</Emphasis>-methyltransferase protein expression in renal cell cancer     

Jun Zhang  Xin-you Xie  Su-wen Yang  Jin Wang  Chao He 《Journal of Zhejiang University. Science. B》2010,11(2):136-143

Objective: To understand the function of nicotinamide N-methyltransferase (NNMT) protein as tumor biomarker in renal carcinoma. Methods: Recombinant NNMT protein was used to prepare monoclonal antibodies by hybridoma technique. The diagnostic and prognostic function of NNMT protein in renal carcinoma was evaluated by analyzing 74 renal cancer tissues through immunohistochemical staining for NNMT by using the prepared antibodies. Results: Two hybridomas named 2F8 and 1E7 stably secreting the monoclonal antib...  相似文献   

Interleukin-6 Perpetrator of the COVID-19 Cytokine Storm     

Jyoti Shekhawat  Kavya Gauba  Shruti Gupta  Purvi Purohit  Prasenjit Mitra  Mahendra Garg  Sanjeev Misra  Praveen Sharma  Mithu Banerjee 《Indian journal of clinical biochemistry : IJCB》2021,36(4):440

COVID-19 has emerged as a global pandemic. It is mainly manifested as pneumonia which may deteriorate into severe respiratory failure. The major hallmark of the disease is the systemic inflammatory immune response characterized by Cytokine Storm (CS). CS is marked by elevated levels of inflammatory cytokines, mainly interleukin-6 (IL-6), IL-8, IL-10, tumour necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). Of these, IL-6 is found to be significantly associated with higher mortality. IL-6 is also a robust marker for predicting disease prognosis and deterioration of clinical profile. In this review, the pivotal role played by IL-6 in the immuno-pathology of COVID-19 has been illustrated. The role of IL-6 as a pleiotropic cytokine executing both pro and anti-inflammatory activities has been reviewed. ADAM 10, a metalloproteinase switches the anti-inflammatory pathway of IL-6 to pro inflammatory hence blocking the action of ADAM 10 could be a new therapeutic strategy to mitigate the proinflammatory action of IL-6. Furthermore, we explore the role of anti-IL6 agents, IL-6 receptor antibodies which were being used for autoimmune diseases but now are being repurposed for the therapy of COVID-19.  相似文献   

Production and Purification of Rabbit’s Polyclonal Antibody Against Factor VIII     

Simin Sohrabi  Azim Akbarzadeh  Dariush Norouzian  Ali Farhangi  Mehri Mortazavi  Mohammad Reza Mehrabi  Mohsen Chiani  Zahra Saffari  Soheil Ghassemi 《Indian journal of clinical biochemistry : IJCB》2011,26(4):354-359

The attempt is made to produce recombinant factor VIII but the first step in producing such product is production and purification of rabbit’s polyclonal antibody against factor VIII. The second and third steps involve monoclonal antibody and recombinant factor VIII production. Factor VIII is one of the most important coagulating factor where its deficiency leads to diseases like hemophilia type A or classic. It is an inherited disease. Previously, it was obtained through fractionation of blood plasma of blood donors. After processing, factor VIII could be used to manage such patients. Due to transfer of viral disease like hepatitis and HIV through factor VIII obtained by fractionation, high cost of production, insufficiency of the donors and the process of virus removal, thus production of factor VIII through recombinant technology can be useful and helpful. The reaction between antibody and antigen is one the most specific reaction; therefore, such reaction can be employed to identify factor VIII. Thereby, rabbits were injected several times with adjuvant-linked antigen to produce antibody. The antibody was separated from the blood sample, purified and used to identify factor VIII in the research.  相似文献   

不同免疫功能对有氧运动的反应   总被引：7，自引：0，他引：7  

谢红  郝选明 《天津体育学院学报》2006,21(3):246-248

通过观察有氧训练过程中SI（T淋巴细胞刺激指数）及血清中IgA、IgG、IgM抗体的应答与适应过程，比较细胞免疫和体液免疫对有氧运动的不同反应，揭示有氧运动下人体免疫机能的变化规律及可能机理。以16名非体育专业的普通大学女生为受试者，进行8周有氧训练。结果显示：（1）作为对有氧运动的应答，细胞免疫功能出现明显应答反应，且运动后1h应答恢复速度加快，至第8周基本完全恢复；而体液免疫对有氧运动的应答反应不明显。（2）作为对有氧运动的适应，体液免疫功能适应明显滞后于细胞免疫功能。（3）总体来看，人体不同免疫功能在训练中都有为期4周的免疫低下期，随后逐渐调整恢复．这与神经-免疫-内分泌网络相互调节、相互作用有关。  相似文献   

Immunodiagnosis of tuberculosis: An update     

A. S. Bhatia  Satish Kumar  B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2003,18(2):1-5

Tuberculosis is still a major health problem in most developing countries and its incidence is rising in many developed countries. This resurgence has been attributed to the HIV epidemic and TB has been declared as a global health emergency by WHO in 1993. The diagnosis of tuberculosis mainly depends upon initial clinical suspicion and radiographic findings with subsequent bacteriological confirmation by sputum smear examination and culture. Lack of sensitivity in smear examination, non specificity of radiological findings, extended tum around time ofMycobacterium tuberculosis culture and difficulties in diagnosing paucibacillary, childhood and extrapulmonary tuberculosis has necessitated to explore the utility of immunodiagnosis of tuberculosis as a convenient and cost effective test to supplement clinical information for definite diagnosis. Many commercial tests are available in the market for diagnosis of TB. Most of these tests are based on the detection of IgG, IgA and IgM antibodies to specific mycobacterial antigen or mixture of antigens. Indigenous immunoassay systems have explored excretory-secretory ES-31 mycobacterial antigen for immunodiagnosis of TB. Many a time there is lack of consistent elevation in all the three Ig classes in active infection thus making it more important to determine the ideal antibody isotype assay for reliable diagnosis of tuberculosis and to save the costs of the patient for unnecessary investigations.  相似文献   

伤寒免疫学诊断方法的研究现状     

鲍行豪 《科技通报》1991,7(6):354-359

对近年伤寒免疫学诊断方法结合作者的研究工作进行了评价。伤寒免疫学诊断可分检测早期病人体液中抗原及测定患者血清中抗体两个方面。检测抗原以SPA-CoA与ELISA(包括Dot-ELISA)较为敏感和特异,而DRLAT则有简便的优点。测定抗体以PHA最为简便与快速。使用LPS-PHA可作伤寒的早期快速诊断,而Vi-PHA可作伤寒病后带菌者的初筛。ELISA有较PHA更高的敏感性,其中LPS-IgM-ELISA亦可作伤寒病人的早期诊断,而LPS-IgG-ELISA可用于该病的追溯诊断和血清流行病学调查。Vi-ELISA还可作伤寒病后带菌者初筛。不同条件实验室可根据取得试剂情况选择使用。  相似文献