Free DNA – new potential analyte in clinical laboratory diagnostics?     

Wagner J 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2012,22(1):24-38

The existence of cell free DNA in the human circulatory system has been known since the 1950s, however, intensive research in this area has been conducted for the last ten years. This review paper brings a short overview of the existing literature concerning the cell free DNA research in various clinical fields and pathological states and considers the application possibilities of this new analyte in clinical laboratory diagnostics. At the moment, cell free DNA is most widely used for the purpose of non-invasive prenatal diagnosis of fetal sex or fetal RhD status. The recent discovery of epigenetic changes in placental/fetal DNA and the detection of fetal/placental-specific RNAs have made it possible to use this technology in all pregnancies irrespective of the gender of the fetus. With the application of new techniques such as next generation sequencing, digital PCR and mass spectrometry, it is now possible to detect very small amounts of specific DNA in the presence of excess of other nonspecific nucleic acids. Second most probable application is in oncology, where detection and monitoring of tumors is now possible by the detection of tumor-derived nucleic acids. Third promising field for near future implementation of this analyte is transplantation medicine, where free DNA level could serve as a marker of transplant rejection. Before any further utilization of this new biomarker, pre-analytical and analytical aspects of free DNA analysis remain to be standardized. In the field of noninvasive prenatal diagnosis, important ethical, legal and social questions remain to be discussed.  相似文献   

默契造化 与道同机——中国传统哲学思想中的空间观念与中国古代山水画的空间构造     

唐凤鸣 《郴州师范高等专科学校学报》2009,(4):79-84

空间在美术中始终是一个重要的概念,不同的空间观会导致截然不同的关术观;中国山水画的空间处理,是以中国古代空间观为基础的,这种空间观念对中国画家产生了极大的影响,在中国画家眼里空间不仅仅是一种客观存在,而且与人的艺术情感相融相谐,主体融入客体,客体融入主体,从而达到人与自然相通、相合的状态,感受自然、生命、宇宙的交融;中国山水画在处理空间时具有极大的灵活性和机动性,认为空间必须服从画面景物的配置与变化;这种独特的艺术手法,既体现了中国画家独特的观察自然的方法,同时也蕴含着中华民族的审美胸襟,体现了中国人的自然观和空间概念。  相似文献   

引入临床路径理念 提高护生实习质量   总被引：2，自引：0，他引：2  

郑冬云  王道富 《成都教育学院学报》2009,23(7):109-110

目的：将临床路径理念引入实习教学工作中,提高护生实习质量。方法：将120名护生随机分为两组．实验组采用根据临床路径理念制定的实习路径进行教学,对照组采用传统教学法,评价其教学效果。结果：两组护生实习满意度和考核成绩均有显著差异,实验组显著高于对照组。结论：实习路径能显著提高实习质量。  相似文献   

5种特色观赏植物室内环境的适应性研究     

陈建洪  陈必新  练发良 《丽水学院学报》2009,31(2):43-46

主要就朱砂根、红凉伞、大罗伞树、披针叶茴香和小叶蚊母等5种浙西南特色观赏植物进行室内摆放试验,观察其对室内环境的适应性情况。通过3种不同环境下250天的摆放,结果发现大罗伞树在室内的适应性最好,披针叶茴香次之,朱砂根和红凉伞又次之,均比从广东引进的鸭掌木表现出更好的适应性,但小叶蚊母仅能在室内安全摆放30余天,其对室内环境的适应性不如鸭掌木。  相似文献   

用有限元方法分析玻璃载芯片封装中的热力耦合问题     

侯芳  李军浩 《江西电力职业技术学院学报》2009,22(4)

超薄液晶显示器(LCD)模块在经历了热压键合后,由于驱动芯片和玻璃基板之间的温度差异较大,会引起一定程度上的翘曲,并最终导致连接点界面分层现象的发生,影响器件连接可靠性。用实验的方法对比进行研究比较困难,我们用有限元数值模拟的方法并选择通用软件ANSYS对这一现象进行了分析;并详细分析了键合压力、温度、基板温度和IC/玻璃厚度对翘曲的影响。从分析中得知,导致玻璃翘曲的主要原因是键合温度。  相似文献   

视觉设计在数字博物馆中的应用价值     

张浩达  刘江娜  张欣 《数字图书馆论坛》2010,(1):68-82

数字博物馆不同于传统的实体博物馆,随着新媒体技术的发展与应用,博物馆中展品展示对于视觉上的要求更加多元化,视觉设计在数字博物馆的建设中成为尤为重要的一个环节。文章阐述了视觉设计在数字博物馆中的价值、功能及作用,从数字博物馆网站用户界面、展品展示、互动娱乐三方面分析了视觉设计的应用价值,并提出了数字博物馆视觉设计中应该注意的问题。  相似文献   

Barriers and enablers to clinical fieldwork education in rural public and private allied health practice     

Phoebe Maloney  Karen Stagnitti  Adrian Schoo 《高等教育研究与发展》2013,32(3):420-435

There is a need to maximise rural clinical fieldwork placement to build health workforce capacity. This study investigated allied health professionals' (AHPs) experience of supervising students as part of work-integrated learning in public and private rural health settings. An anonymous postal questionnaire with 30 questions was used to collect quantitative and qualitative data about the barriers and enablers that AHPs encounter when supervising students in their clinical setting. A total of 113 public and private AHPs from Southwest Victoria, Australia, returned the questionnaire. The AHPs were trained in the disciplines of occupational therapy, physiotherapy, speech pathology, dietetics, podiatry or psychology. The majority of respondents (75%) had previously supervised students. Most respondents had only provided fieldwork education in the public sector. Allied health professionals working in public and private sectors had positive experiences with clinical fieldwork education and often had increased job satisfaction while supervising students. They experienced similar enablers to involvement in clinical fieldwork education programs, however the barriers they encountered were different. The findings highlight the differing issues between rural public and private settings that need to be addressed for successful clinical fieldwork education and work-integrated learning. Strategies to address the identified barriers need to be specific to the work conditions of each setting.  相似文献   

Mini-CEX对专业学位硕士研究生消化内科临床培养效果的研究     

苏思标  郭晓云  陶霖  罗薇  覃山羽  姜海行 《科教文汇》2021,(6)

目的:探讨Mini-CEX在专业学位硕士研究生临床能力培养中的应用效果。方法:选取2019年1月至2019年12月在广西医科大学第一附属医院消化内镜病区轮转的60名专业学位硕士研究生作为研究对象,在入科、轮科满1月、轮科满2月和出科时(满3月)进行Mini-CEX测评,同时结合问卷调查,评判Mini-CEX的教学效果及认可程度。结果:全体研究对象出科时,在病史采集、体格检查、知情告知、临床判断、组织协调、人文关怀和整体评价7个测评项目上的得分,与入科时相比均有明显提高(P<0.01),约93.33%的研究对象认可Mini-CEX教学方法。结论:Mini-CEX是一种简单、易行且有效的教学方法,能够显著提高医学生的临床综合能力,值得在专业学位硕士研究生的临床培养中应用与推广。  相似文献   

浅析招生工作对临床医学专业学位硕士研究生生源质量的保障     

马琳 《科教文汇》2021,(13):103-104

社会对临床医学专业学位研究生的需求日益增长,为选拔优秀生源,招生工作的重点是在复试中多维度地考查学生的理论基础、英语水平、临床实践能力。通过Pear s on相关分析,研究生的入学总成绩与校内临床医学第一阶段考核成绩显著相关,说明可以有效选拔出优秀生源。  相似文献   

唐城墙地基展示坑保存现状调查及其保护方法探讨     

水碧纹  邵振宇  曹明静 《天水师范学院学报》2011,31(5):140-144

针对唐城墙地基展示坑遗址保存现状的调查和治理方法的研究,通过对两段唐城墙地基展示坑保护和展示现状的调查,全面了解遗址面临的主要病害和保护、展示中存在的问题,并结合国内外有关研究,对两段唐城墙遗址的病害治理方法进行探讨,同时还要解决遗址的展陈方式中存在的问题,包括玻璃罩表面结雾、遗址的密封材料等.  相似文献