Leaf and root glucosinolate profiles of Chinese cabbage (Brassica rapa ssp. pekinensis) as a systemic response to methyl jasmonate and salicylic acid elicitation     

Yun-xiang Zang  Jia-li Ge  Ling-hui Huang  Fei Gao  Xi-shan Lv  Wei-wei Zheng  Seung-beom Hong  Zhu-jun Zhu 《Journal of Zhejiang University. Science. B》2015,16(8):696-708

Glucosinolates (GSs) are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid (JA) and salicylic acid (SA) are major regulators of plant response to pathogen attack. However, there is little information about the interactive effect of both elicitors on inducing GS biosynthesis in Chinese cabbage (Brassica rapa ssp. pekinensis). In this study, we applied different concentrations of methyl jasmonate (MeJA) and/or SA onto the leaf and root of Chinese cabbage to investigate the time-course interactive profiles of GSs. Regardless of the site of the elicitation and the concentrations of the elicitors, the roots accumulated much more GSs and were more sensitive and more rapidly responsive to the elicitors than leaves. Irrespective of the elicitation site, MeJA had a greater inducing and longer lasting effect on GS accumulation than SA. All three components of indole GS (IGS) were detected along with aliphatic and aromatic GSs. However, IGS was a major component of total GSs that accumulated rapidly in both root and leaf tissues in response to MeJA and SA elicitation. Neoglucobrassicin (neoGBC) did not respond to SA but to MeJA in leaf tissue, while it responded to both SA and MeJA in root tissue. Conversion of glucobrassicin (GBC) to neoGBC occurred at a steady rate over 3 d of elicitation. Increased accumulation of 4-methoxy glucobrassicin (4-MGBC) occurred only in the root irrespective of the type of elicitors and the site of elicitation. Thus, accumulation of IGS is a major metabolic hallmark of SA- and MeJA-mediated systemic response systems. SA exerted an antagonistic effect on the MeJA-induced root GSs irrespective of the site of elicitation. However, SA showed synergistic and antagonistic effects on the MeJA-induced leaf GSs when roots and leaves are elicitated for 3 d, respectively.  相似文献   

Detection of Bone Metastases in Breast Cancer (BC) Patients by Serum Tartrate-Resistant Acid Phosphatase 5b (TRACP 5b), a Bone Resorption Marker and Serum Alkaline Phosphatase (ALP), a Bone Formation Marker,in Lieu of Whole Body Skeletal Scintigraphy with Technetium99m MDP     

B. K. D. Sarvari  D. Sankara Mahadev  S. Rupa  S. A. Mastan 《Indian journal of clinical biochemistry : IJCB》2015,30(1):66-71

Bone metastases are a serious problem in patients with advanced cancer disease and their presence usually signifies serious morbidity prior to the patient’s death. In breast cancer patients the incidence of bone metastasis is observed to be very high at 70 %, as seen during post-mortem examination. Bone metastasis is difficult to diagnose, treat or follow clinically without radiological tools. This study was designed to evaluate the utility of a novel bone resorption marker–serum tartrate-resistant acid phosphatase 5b (TRACP5b) and the bone formation marker such as serum total alkaline phosphatase (ALP), in comparison with whole body skeletal scintigraphy with Technetium99m MDP for the diagnosis of bone metastases (BM) in breast cancer (BC) patients. This study is intended to help the clinician to diagnose bone metastasis without resorting to radiological tools, as they are not cost effective and carry the risk of radiation. Experimental design: Four groups of samples were analysed. 1st group consists 52 normal female (cancer free women), 2nd group consists 38 BC patients without bone metastasis, 3rd group consists 27 breast cancer patients with limited bone metastasis (3 or less than 3 skeletal lesions) and 4th group consists 35 breast cancer patients with extensive bone metastasis (4 or more than 4 skeletal lesions), conformed by whole body skeletal scintigraphy with Technetium99m MDP. One way ANOVA was used to compare serum TRACP5b and serum ALP among these groups. Both serum TRACP5b and serum ALP are not markedly elevated in limited bone metastasis but are strongly elevated in extensive bone metastasis (p < 0.0001). As seen in this study the biochemical bone resorption marker, serum TRACP5b, abnormally increased in extensive bone metastasis of breast cancer patients and can be used as a specific marker for bone metastasis in lieu of radiological tools.  相似文献   

聚丙烯酸吸水树脂的反相悬浮聚合研究   总被引：14，自引：0，他引：14  

陈时忠  徐伟亮 《科技通报》2001,17(1):52-55

环己烷为连续相，水为分散相，采用反相悬浮聚合法合成了聚丙烯酸高吸水性树脂，研究了碱用量，引发剂用量，交联剂用量及温度等反应条件对树脂物理状态和吸水性能的影响，制得吸水树脂最高可吸水850mL/g，吸0．9％NaCl溶液120mL/g。  相似文献   

乳清酸的单扫示波极谱研究   总被引：1，自引：0，他引：1  

邹洪  袁倬斌 《中国科学院研究生院学报》1996,(1)

乳清酸在０．１ｍｏｌ／ＬＨＣｌＯ４＋０．０２ｍｏｌ／ＬＮａＣｌＯ４底液中，于ＪＰ－２型示波极谱仪上，有一良好的二阶导数峰Ｅｐ＝－０．８６０±０．０１Ｖ（ＳＣＥ），浓度在１×１０－７～６×１０－５ｍｏｌ／Ｌ范围内，与峰高成线性关系，检测限为７．５×１０－８ｍｏｌ／Ｌ．实验证明，电极过程为具有催化性质的双电子可逆过程．  相似文献   

微波辐射合成肉桂酸     

宋桂苓 《中国科技信息》2005,(17):93

本文介绍了微波辐射合成肉桂酸的方法,该法与传统加热法相比具有简便快捷、产率和纯度高、对环境污染小的优点。  相似文献   

Serum glycoconjugates and ceruloplasmin in cancer of uterine cervix     

Sharmila Upadhya  Subramanya Upadhya  K. S. Prabhu 《Indian journal of clinical biochemistry : IJCB》2002,17(1):20-24

The present study was done to assess the levels of glycoconjugates and ceruloplasmin in sera of patients with cervical cancer. Serum hexoses, hexosamines, sialic acid and fucose are elevated in a variety of inflammatory and neoplastic conditions. All the glycoconjugates, except fucose were increased in serum of patients compared to controls. Also, hexoses and sialic acid levels were high in patients with later stages of cancer compared to patients with early stage disease (P=<0.0001, P=0.03). Serum ceruloplasmin was increased in patients with early stage cancer (51.5mg/dl) and with late stage cancer (61mg/dl) compared to controls (38mg/dl). The elevated glycoconjugates may be the result of inflammatory reaction associated with neoplasia, as serum ceruloplasmin (an acute phase reactant) is also increased in these patients.  相似文献   

SINGLE-SWEEP OSCILLOPOLARO-GRAPHIC ANALYSIS OF P-NITROBENZOIC ACID     

Yuan Zhuo-bin  Han Feng-mei  Chen Yong  Wu Yan-xia 《中国科学院研究生院学报》1995,(1)

ＳＩＮＧＬＥ－ＳＷＥＥＰＯＳＣＩＬＬＯＰＯＬＡＲＯ－ＧＲＡＰＨＩＣＡＮＡＬＹＳＩＳＯＦＰ－ＮＩＴＲＯＢＥＮＺＯＩＣＡＣＩＤ￥ＹｕａｎＺｈｕｏ－ｂｉｎ；ＨａｎＦｅｎｇ－ｍｅｉ；ＣｈｅｎＹｏｎｇ；ＷｕＹａｎ－ｘｉａ（ＧｒａｄｕａｔｅＳｃｈｏｏｌ，Ｃｈｉｎ...  相似文献   

关于氯甲基膦酸性质的研究   总被引：1，自引：0，他引：1  

黄来旺 《科技通报》1993,9(5):303-305

介绍氯甲基膦酸的合成方法、纯品的制备和主要用途以及氯甲基膦酸的物理化学性质.重点是对该化合物的酸性进行了研究,利用电位滴定法绘出了滴定曲线.研究表明,氯甲基膦酸为二元酸,可以进行二级电离.在测定温度(27℃)下,其浓度平衡常数Kα_1和Kα_2分别为1.29×10~(-2)、6.82×10~(-7);活度平衡常数K’α_1和K’α_2分别为1.26×10~(-2)和6.51×10~(-7).  相似文献   

近年来京津地区酸雨形势变化的特点分析——气溶胶影响的探讨   总被引：8，自引：0，他引：8  

汤洁  徐晓斌  巴金  王淑凤  丁国安 《中国科学院研究生院学报》2007,24(5):667-673

本文利用2000年以来中国气象局的酸雨观测站网的观测资料，分析了近年来我国酸雨形势出现的一些新的变化，其中京津地区近年来酸雨程度与2000年前相比明显地逐年加重趋势最值得关注。本文着重分析了京津地区的酸雨程度的长期变化趋势，指出：为了迎接奥运会近年来北京等地加大了空气污染的治理力度，大气颗粒物总浓度的逐年下降，一次颗粒物如扬尘、工业粉尘对降水酸性的中和能力减弱，二次颗粒物如硫酸盐、硝酸盐等浓度和比例的升高，可能是近年来京津地区降水酸性明显增加的重要原因；这种情况提示我们，区域降水酸性对近年来采取的相关环境治理措施具有敏感的响应。  相似文献   

Synthesis and Characterization of Poly（ N-Vinyl-2-Pyrrolidone／Itaconic Acid） Hydrogel     

郭锦棠  李伶  李雄勇  刘冰 《天津大学学报(英文版)》2004,10(3):176-178

With N-vinyl-2-pyrrolidone (NVP) and itaconic acid ( IA ), poly ( N-vinyl-2-pyrrolidone/itaconic acid) [ P(NVP/IA) ] hydrogel was synthesized by free radical solution polymerization. The structure of this P(NVP/IA) was characterized by IR. Effects of concentration of itaconic acid, amount of cross-link agent,N, N‘-methylene-bis-acrylamide, reaction temperature, and time on properties of swelling ratio (SR) of the hydrogel were investigated. The results show that the best swelling property of the hydrogel is obtained at 50 ℃ and 1.5 h. pH sensitivity increases as the concentration of itaconic acid in the hydrogel system increases.Swelling ratio of the hydrogel decreases as the amount of cross-link agent increases.  相似文献