基于CiteSpaceII的植物科学知识图谱可视化分析     

刘玉博 《情报探索》2013,(11):17-21

以WebofScience数据库中《植物细胞》杂志自1989年创刊号到2012年底的所有文献作为研究对象,运用CiteS-paceII软件进行文献共引分析和共词分析。以知识可视化图谱方式展现20多年来植物科学领域的研究机构、知识基础、研究热点及研究前沿。  相似文献   

Parthenolide inhibits proliferation of vascular smooth muscle cells through induction of G0/G1 phase cell cycle arrest     

Shao-xiang Weng  Mei-hua Sui  Shan Chen  Jian-an Wang  Geng Xu  Ji Ma  Jiang Shan  Lu Fang 《Journal of Zhejiang University. Science. B》2009,10(7):528-535

Objective  This study is to determine the effect of the natural product parthenolide, a sesquiterpene lactone isolated from extracts of the herb Tanacetum parthenium, on the proliferation of vascular smooth muscle cells (VSMCs). Methods  Rat aortic VSMCs were isolated and cultured in vitro, and treated with different concentrations of parthenolide (10, 20 and 30 μmol/L). [³H]thymidine incorporation was used as an index of cell proliferation. Cell cycle progression and distribution were determined by flow cytometric analysis. Furthermore, the expression of several regulatory proteins relevant to VSMC proliferation including IκBα, cyclooxygenase-2 (Cox-2), p21, and p27 was examined to investigate the potential molecular mechanism. Results  Treatment with parthenolide significantly decreased the [³H]thymidine incorporation into DNA by 30%∼56% relative to control values in a dose-dependent manner (P<0.05). Addition of parthenolide also increased cell population at G₀/G₁ phase by 19.2%∼65.7% (P<0.05) and decreased cell population at S phase by 50.7%∼84.8% (P<0.05), which is consistent with its stimulatory effects on p21 and p27. In addition, parthenolide also increased IκBα expression and reduced Cox-2 expression in a time-dependent manner. Conclusion  Our results show that parthenolide significantly inhibits the VSMC proliferation by inducing G₀/G₁ cell cycle arrest. IκBα and Cox-2 are likely involved in such inhibitory effect of parthenolide on VSMC proliferation. These findings warrant further investigation on potential therapeutic implications of parthenolide on VSMC proliferation in vivo. Project (No. 491020-W50315) supported by the Foundation of the Health Bureau of Zhejiang, China  相似文献   

Knockdown of OLA1, a regulator of oxidative stress response, inhibits motility and invasion of breast cancer cells     

Jia-wei Zhang  Valentina Rubio  Shu Zheng  Zheng-zheng Shi 《Journal of Zhejiang University. Science. B》2009,10(11):796-804

To explore the role of a novel Obg-like ATPase 1 (OLA1) in cancer metastasis, small interference RNA (siRNA) was used to knockdown the protein, and the cells were subjected to in vitro cell migration and invasion assays. Knockdown of OLA1 significantly inhibited cell migration and invasion in breast cancer cell line MDA-MB-231. The knockdown caused no changes in cell growth but affected ROS production. In wound-healing assays, decreased ROS in OLA1-knockdown cells were in situ asso-ciated with the cells' decreased motile morphology. Further, treatment of N-acetylcysteine, a general ROS scavenger, blunted the motility and invasiveness of MDA-MB-231 cells, similar to the effect of OLA1-knockdown. These results suggest that knock-down of OLA1 inhibits breast cancer cell migration and invasion through a mechanism that involves the modulation of intracel-lular ROS levels.  相似文献   

JTable组件的深入剖析     

吴闻 《科技广场》2012,(3):39-44

JTable组件是一个综合性的Java界面开发组件,内部包含一套独立的消息传递机制,以协调整个组件的运作。文中对JTable组件进行了深入的分析,介绍了JTable组件采用的设计模式、消息的传递方法、JTable的使用、TableModel的常用方法、如何向JTable传递消息及如何实现对JTable的控制。最后,介绍了单元渲染器和编辑器的实现和使用。  相似文献   

Media Use During a Crisis     

《普罗米修斯》2012,30(3):201-207

After learning about the crisis of September 11, Americans overwhelmingly turned to television for more information. However, people used multiple sources of information. The Web and e-mail appear to have played important but secondary roles to television and the telephone. Overall, the media functioned well in meeting the extraordinary demands for information and communication.  相似文献   

胚胎干细胞的鉴定方法研究进展   总被引：1，自引：0，他引：1  

王正朝  王锋  庞训胜 《安徽科技学院学报》2004,18(4):1-5

胚胎干细胞的鉴定工作对于研究和利用胚胎干细胞具有重大的意义.本文综述了利用胚胎干细胞的形态特征、生长特性、染色体结构、多能性和全能性,以及免疫学方法鉴定胚胎干细胞的研究情况,并介绍了几种常用的鉴定过程.  相似文献   

新兴技术竞争优势的测度方法研究——基于四象限图示和雷达图示分析方法     

栾春娟 《科学与管理》2016,(5):42-48

探索新兴技术竞争优势的测度方法,有助于我们把握技术前沿的竞争态势,推动科技成果的转化。采用四象限图示分析方法和雷达图示分析方法,借助Relecura专业知识产权分析平台,以细胞免疫治疗为实证,对技术主体的综合竞争优势和高产机构在主要技术领域、子领域、IPC代码的竞争优势进行了分析。就专利技术战略布局的前瞻性指标而言,美国国立卫生研究院和美国卫生与公众服务部两个机构最具有前瞻性。就创造高价值专利的执行力而言,美国国立卫生研究院和Celera公司具有相对比较强的创造高价值专利技术的执行力。高产机构在主要技术领域的竞争优势各异,美国卫生与公众服务部在更多的技术子领域和IPC代码具有优势。  相似文献   

DNA-damage response network at the crossroads of cell-cycle checkpoints,cellular senescence and apoptosis   总被引：2，自引：0，他引：2  

Schmitt E  Paquet C  Beauchemin M  Bertrand R 《Journal of Zhejiang University. Science. B》2007,8(6):377-397

Tissue homeostasis requires a carefully-orchestrated balance between cell proliferation, cellular senescence and cell death. Cells proliferate through a cell cycle that is tightly regulated by cyclin-dependent kinase activities. Cellular senescence is a safeguard program limiting the proliferative competence of cells in living organisms. Apoptosis eliminates unwanted cells by the coordinated activity of gene products that regulate and effect cell death. The intimate link between the cell cycle, cellular senescence, apoptosis regulation, cancer development and tumor responses to cancer treatment has become eminently apparent. Extensive research on tumor suppressor genes, oncogenes, the cell cycle and apoptosis regulatory genes has revealed how the DNA damage-sensing and -signaling pathways, referred to as the DNA-damage response network, are tied to cell proliferation, cell-cycle arrest, cellular senescence and apoptosis. DNA-damage responses are complex, involving ＂sensor＂ proteins that sense the damage, and transmit signals to ＂transducer＂ proteins, which, in turn, convey the signals to numerous ＂effector＂ proteins implicated in specific cellular pathways, including DNA repair mechanisms, cell-cycle checkpoints, cellular senescence and apoptosis. The Bcl-2 family of proteins stands among ＂the mos＂t crucial regula＂tors of apop＂tosis and performs vi＂tal func＂tions in deciding whether a cell will live or die after cancer chemotherapy and irradiation. In addition, several studies have now revealed that members of the Bcl-2 family also interface with the cell cycle, DNA repair/recombination and cellular senescence, effects that are generally distinct from their function in apoptosis. In this review, we report progress in understanding the molecular networks that regulate cell-cycle checkpoints, cellular senescence and apoptosis after DNA damage, and discuss the influence of some Bcl-2 family members on cell-cycle checkpoint regulation.  相似文献   

Artesunate inhibits growth and induces apoptosis in human osteosarcoma HOS cell line in vitro and in vivo     

Xu Q  Li ZX  Peng HQ  Sun ZW  Cheng RL  Ye ZM  Li WX 《Journal of Zhejiang University. Science. B》2011,12(4):247-255

This paper aims to investigate the effects of artesunate (ART) on growth and apoptosis in human osteosarcoma HOS cell line in vitro and in vivo and to explore the possible underlying mechanisms. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The induction of apoptosis was detected by light and transmission electron microscopy and flow cytometry. Western blot analysis was used to investigate the related mechanisms. Nude mice were further employed to investigate the antitumour activity of ART in vivo. MTT assay results demonstrated that ART selectively inhibits the growth of HOS cells in a dose- and time-dependent manner. Based on the findings of light and transmission electron microscopy, Hoechst 33258 staining, and fluorescein isothiocyanate (FITC)-annexin V staining, the cytotoxicity of ART in HOS cells occurs through apoptosis. With ART treatment, cytosolic cytochrome c was increased, Bax expression was gradually upregulated, Bcl-2 expression was downregulated, and caspase-9 and caspase-3 were activated. Thus, the intrinsic apoptotic pathway may be involved in ART-induced apoptosis. Cell cycle analysis by flow cytometry indicated that ART may induce cell cycle arrest at G₂/M phase. In nude mice bearing HOS xenograft tumours, ART inhibited tumour growth and regulated the expressions of cleaved caspase-3 and survivin, in agreement with in vitro observations. ART has a selective antitumour activity against human osteosarcoma HOS cells, which may be related to its effects on induction of apoptosis via the intrinsic pathway. The results suggest that ART is a promising candidate for the treatment of osteosarcoma.  相似文献   

Defensin γ-thionin from Capsicum chinense improves butyrate cytotoxicity on human colon adenocarcinoma cell line Caco-2     

《Electronic Journal of Biotechnology》2021

BackgroundButyrate is a histone deacetylase inhibitor that induces apoptosis and inhibits cell proliferation of colorectal cancer cells. To improve its anticancer activity, butyrate has been evaluated mixed with drugs and different molecules. Plant antimicrobial peptides are attractive anticancer alternative molecules because they show selective cytotoxic activity against different cancer cell lines. In this work, we explore if the plant defensin γ-thionin (Capsicum chinense) can improve butyrate activity on Caco-2 cell line and we also determined the mechanism of death activated.ResultsThe combined treatment of γ-thionin (3.5 µM) and butyrate (50 mM) showed higher cytotoxicity on Caco-2 cells with respect to single treatments. Also, the combined treatment reduced cell proliferation and exhibited a higher rate of apoptosis than single treatments. Combined treatment induced caspases 8 and 9 activation to an extent comparable with that of butyrate while γ-thionin did not activate caspases. Additionally, reactive oxygen species generation preceded the onset of apoptosis, and superoxide anion production was higher in cells treated with the combined treatment.ConclusionsThe γ-thionin from Habanero chili pepper improved the butyrate cytotoxicity on Caco-2 cells. This effect occurred through apoptosis induction associated with reactive oxygen species production. Therefore, the combination of butyrate with cytotoxic antimicrobial peptides could be an attractive strategy for cancer therapy.How to cite: Velázquez-Hernández ME, Ochoa-Zarzosa A, López-Meza JE, Defensin γ-thionin from Capsicum chinense improves butyrate cytotoxicity on human colon adenocarcinoma cell line Caco-2. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.04.009  相似文献