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81.
为探求淮南区域1煤层瓦斯治理技术,对新集二矿1煤层首采工作面采取了不同的瓦斯治理手段,通过分析比较工作面回采期间风排瓦斯、底板巷高负压抽采瓦斯、高抽巷抽采瓦斯、风机巷顺层孔抽采瓦斯以及采空区埋管抽采瓦斯,初步得到用于新集二矿1煤层瓦斯治理有效的技术手段,以为以后1煤层的安全开采提供参考。 相似文献
82.
为了研究口模出口高度变化对螺杆分配机头内部流场和挤出均匀性的影响,参数化有限元分析技术贯穿于螺杆分配机头CAE仿真的全过程,采用VISUAL C++和APDL语言开发出专用有限元前处理程序和特殊加载宏,调用ANSYS对螺杆分配机头流道内部流场进行三维有限元模拟与分析。分析结果表明:口模出口高度变小将增加流道内的流动阻力,显著增大狭缝流道内各部分区域过渡截面中间层节点的压力值和口模出口的挤出速度值。当口模出口高度调节较大时,有必要重新调节阻尼块以提高速度分布的均匀性。 相似文献
83.
84.
Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock 总被引:1,自引:0,他引:1
Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mueosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Spragne-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluo-rescein-iso-tbiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P<0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P<0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma. 相似文献
85.
进入21世纪,远程开放教育作为构筑知识经济时代人们终身学习体系的主要手段被确定下来,为一切有志终身学习的人敞开了大门,并为他们提供了各种学习的方便条件。在这个学习过程中,由于师生准永久性地分离,以往那些学生学有所成的必要条件,例如:学习个性化的建立、学习模式的适应、学业的完成等都需要由学习支持服务系统来提供保障。这就需要我们分析学习空间的服务需求,探讨支持服务的运行机制,绘制远程开放教育的学习支持服务系统的流程图,以适应不同地区、不同层次、不同内容的支持服务。 相似文献
86.
社会分层、社会流动与高校教育之间存在着密切的关系,高等教育在现代社会中已逐渐成为向上流动的一个决定性因素。高校招生收费过高带来的一系列社会问题,尤其是对社会流动带来的负面影响说明,在注重高等教育发展的同时,绝对不能忽视社会流动的公平性:为了使社会流动更加公平,高校招生收费一定不能过高。 相似文献
87.
Objective: To investigate the cytokine response pattern (IL-4/IFN-γ) in infants with RSV infections and bronchiolitis during the acute phase. Methods: Four-color flow cytometry was used to measure intracellular IL-4 and IFN-γ expressions in peripheral blood CD3+ and CD8+ lymphocytes from RSV-infected and bronchiolitis infants. Serum IL-4 and IFN-γ levels were also determined. Results: RSV-infected and bronchiolitis infants showed no statistical differences from not-RSV-infected or pneumonia infants and control in the frequency of IL-4 and IFN-γ expressions in CD3+CD8- lymphocytes, showed no obvious Th1/Th2 imbalance, while IFN-γ was expressed much more frequently in CD3+CD8+ lymphocytes. Systematically, RSV-infected and bronchiolitis infants showed much lower levels of serum IL-4 and IL-4/IFN-γ ratios and much higher serum IFN-γ levels than control. However, there were no statistical differences in the above three indices between RSV-infected and not-RSV infected infants or between bronchiolitis and pneumonia infants, except that bronchiolitis infants had a higher level of serum IFN-γ than pneumonia infants statistically. Conclusions: There is no type-2 cytokine response predominance in the acute phase of RSV infection and bronchiolitis. IL-4 production is suppressed and IFN-γ production upregulated, the latter being most prominent in bronchiolitis infants. 相似文献
88.
89.
“双一流”战略是中国高等教育发展史上一个里程碑式的战略举措。该战略提出后,中国高校亟需高层次人才加强自身学科建设。在此背景下,高校高层次人才引进和流动日益频繁,这也为高校与高层次人才流动博弈提供了条件。本文创新点是根据理性人假设,从混合策略纳什均衡博弈和委托-代理博弈角度出发,多维度分析了高校与高层次人才之间的流动博弈。博弈研究发现高校与高层次人才博弈存在博弈均衡解问题,高层次人才流动还与薪酬奖励、城市发展、家庭幸福和科研平台有直接关系。本文立足我国高校发展现状并结合博弈研究结果提出了相应的政策建议。 相似文献
90.
Sanju Jalla Sunil Sazawal Saikat Deb Robert E. Black Maharaj K. Bhan 《Indian journal of clinical biochemistry : IJCB》2002,17(1):69-74
The development of monoclonal antibodies combined with flow cytometry has revolutionized the analysis of lymphocyte subsets.
These newer methods using the Q-prep leucocyte preparation system require only 1–2 ml of blood as compared to 10 ml required
traditionally. One of the main impediments in the use of this superior technology in Indian laboratories has been the high
cost of reagents. This study evaluated methods to reduce the cost of assays. In the first experiment from 26 healthy subjects,
2ml venous blood samples in EDTA (ethylenediamine tetra-acetate) were obtained. Each sample was divided into two equal portions,
one portion was stained using diluted monoclonal antibody, whereas the other portion was stained using standard concentrations
of antibodies. In the second experiment, blood samples from 12 subjects were again divided into 2 portions; one portion of
each pair was processed using commercial Q-prep reagents while the other portion was processed using our own reagents. In
the first experiment, which evaluated use of a diluted antibody against the standard recommended concentrations, a 5-tube
panel that estimated CD3, CD4, CD8, CD20 was used. In the second experiment CD3, CD4 and CD8 were estimated. The total cost
per sample for a 5-panel estimation was however reduced from $39.11 to $1.10.
Given the proven advantages of using a whole blood stain-lyse method for T cell subset estimations, its use should be encouraged
in developing country settings. With the suggested methods the whole blood Q-prep could be performed at appreciably reduced
costs, without loss in precision. 相似文献