Clones identification of Sequoia sempervirens (D.Don) Endl.in Chile by using PCR-RAPDs technique     

Manuel TORAL IBAEZ  Margarita CARU  Miguel A.HERRERA  Luis GONZALEZ  Luis M.MARTIN  Jorge MIRANDA  Rafael M.NAVARRO-CERRILLO 《Journal of Zhejiang University. Science. B》2009,10(2)

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D.Don) Endl.in Chile.Ten (out of 34) clones from introduction trial located in Voipir-Viilarrica,Chile,were studied.The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.The PCR tests were carried out employing 10-mer random primers.The amplification products were detected by electrophoresis in agarose gels.Forty nine polymorphic bands were obtained with the selected primers (BG04,BF07,BF 12,BF13,and BF 14) and were ordered according to their molecular size.The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).Of the primers tested,5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism.A total of 49 polymorphic RAPD bands were detected out of 252 bands.The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones,suggesting a geographic relationship.The results indicate that the RAPD markers permitted the identification of the assayed clones,although they are derived from the same geographic origin.  相似文献   

Clones identification of Sequoia sempervirens (D. Don) Endl. in Chile by using PCR-RAPDs technique     

Manuel Toral Ibañez  Margarita Caru  Miguel A. Herrera  Luis Gonzalez  Luis M. Martin  Jorge Miranda  Rafael M. Navarro-Cerrillo 《Journal of Zhejiang University. Science. B》2009,10(2):112-119

A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D. Don) Endl. in Chile. Ten (out of 34) clones from introduction trial located in Voipir-Villarrica, Chile, were studied. The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction. The PCR tests were carried out employing 10-mer random primers. The amplification products were detected by electrophoresis in agarose gels. Forty nine polymorphic bands were obtained with the selected primers (BG04, BF07, BF12, BF13, and BF14) and were ordered according to their molecular size. The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA). Of the primers tested, 5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism. A total of 49 polymorphic RAPD bands were detected out of 252 bands. The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones, suggesting a geographic relationship. The results indicate that the RAPD markers permitted the identification of the assayed clones, although they are derived from the same geographic origin. Project (No. CONICYT-FONDEF PROYECT D01 I 1008) supported by the National Commission of Science and Research of Chile  相似文献   

分子标记及其在甘薯种质资源和遗传育种中的应用     

邓平  李仁全 《四川职业技术学院学报》2007,17(3):21-123

植物分子生物学和植物生物技术利用分子标记技术得到了大力发展,分子标记的创新、发展与应用对其起了积极的推动作用,本文就RFLP、AFLP、RAPD、DAF、SSR、CAPS分子标记的原理与技术及其在甘薯种质资源和遗传育种中的应用作一介绍  相似文献   

具有势函数V(r)=A1r-10-A2r-6+A3r2分子晶体的Hamiltonian本征方程的精确解     

周国中 《金华职业技术学院学报》2003,3(2):39-41,61

本文采用连分法得到描述分子晶体中分子之间的相互作用势V(r)=Atr-10-A2r-6+A3r2(A1,A2,A3＞0)的分子晶体的Hamiltonian算子的精确的能量本征值和能量本征函数.  相似文献   

研究生细胞与分子免疫学课程的改革探索     

曾常茜  张庆镐 《教育教学论坛》2019,(27):122-123

课程改革是培养研究生创新能力,提高教学质量的重要途径。本文从课程教学内容、教学模式和教学资源等方面入手,对研究生细胞与分子免疫学课程进行改革和实践,取得了很好的教学效果,实现了知识传承与知识创新的融合,知识的学习与学生素质、能力的培养同步,对于提高研究生教学质量有着借鉴意义。  相似文献   

Exploration of Circulating Tumour Cell (CTC) Biology: A Paradigm Shift in Liquid Biopsy     

Anshika Chauhan  Rajandeep Kaur  Sushmita Ghoshal  Arnab Pal 《Indian journal of clinical biochemistry : IJCB》2021,36(2):131

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Molecular dynamics simulation of the interactions between EHD1 EH domain and multiple peptides     

Hua Yu  Mao-Jun Wang  Nan-Xia Xuan  Zhi-Cai Shang  Jun Wu 《Journal of Zhejiang University. Science. B》2015,16(10):883-896

Objective: To provide essential information for peptide inhibitor design, the interactions of Eps15 homology domain of Eps15 homology domain-containing protein 1 (EHD1 EH domain) with three peptides containing NPF (asparagine-proline-phenylalanine), DPF (aspartic acid-proline-phenylalanine), and GPF (glycine-proline-phenylalanine) motifs were deciphered at the atomic level. The binding affinities and the underlying structure basis were investigated. Methods: Molecular dynamics (MD) simulations were performed on EHD1 EH domain/peptide complexes for 60 ns using the GROMACS package. The binding free energies were calculated and decomposed by molecular mechanics/generalized Born surface area (MM/GBSA) method using the AMBER package. The alanine scanning was performed to evaluate the binding hot spot residues using FoldX software. Results: The different binding affinities for the three peptides were affected dominantly by van der Waals interactions. Intermolecular hydrogen bonds provide the structural basis of contributions of van der Waals interactions of the flanking residues to the binding. Conclusions: van der Waals interactions should be the main consideration when we design peptide inhibitors of EHD1 EH domain with high affinities. The ability to form intermolecular hydrogen bonds with protein residues can be used as the factor for choosing the flanking residues.  相似文献   

红豆杉科及三尖杉科的分子系统发育——兼论竹柏属的系统位置     

汪小全  舒艳群 《中国科学院研究生院学报》2000,38(3):201-210

测定了红豆杉科、三尖杉科、罗汉松科及松科共9属13种植物的matK基因序列,发现3′端的单碱基插入造成mat k基因在这些类群间有较大的长度变异(1488∽1548bp),其它插入或缺失的长度为3、6或9bp(白豆杉中有一个长为27bp的缺失),且绝大多数插入或缺失事件具有系统发育信息。matK基因中,密码子第1、2及3位的变异率较为相近,平均同义替代率约为平均非同义替代率的2倍。以松科2属3种植物为外类群,运用PAUP软件进行的系统发育分析仅得到一棵最简约树(步长为895,CI=0．850,RI=0．876),该简约树的各分支均得到bootstrap分析的极强支持。结果表明：红豆杉科与三尖杉科均为单系群,二者互为姐妹群;白豆杉属与穗花杉属为红豆杉科中两个很自然的属,白豆杉属为红豆杉属的姐妹群,穗花杉属为榧树属的姐妹群。竹柏属与罗汉松属聚为一支,二者间的亲缘关系得到boot-strap分析的100％支持,从分支分类学及遗传距离角度,作者不赞同将竹柏属提升为科。  相似文献   

Current Situation of Molecular Diagnostic Assay in Iran: Is it Necessary to Revise of Health Surveillance System at Laboratory Level?     

Massoud Hajia  Amir Sohrabi 《Indian journal of clinical biochemistry : IJCB》2021,36(2):250

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基于分子信标的DNA自组装模型研究     

张文逸  张喆  李楠 《中国科教创新导刊》2014,(13):115-116

分子信标操作简单且不必与未反应的探针分离即可进行实时检测,将分子信标与自组装结合,将为DNA计算研究提供新的思路及方法.本文介绍了基于分子信标的DNA自组装模型的算法思想,阐明了分子信标设计要点,描述了生物实现步骤,并对如何提高分子信标的检测准确性做了展望.  相似文献