Immobilization of foreign protein into polyhedra of Bombyx mori nucleopolyhedrovirus (BmNPV)     

Xing-wei Xiang  Rui Yang  Lin Chen  Xiao-long Hu  Shao-fang Yu  Cui-ping Cao  Xiao-feng Wu 《Journal of Zhejiang University. Science. B》2012,13(2):111-117

In the late phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection, a large amount of polyhedra appear in the infected cell nucleolus, these polyhedra being dense protein crystals protecting the incorporated virions from the harsh environment. To investigate whether the foreign protein could be immobilized into the polyhedra of BmNPV, two recombinant baculoviruses were generated by a novel BmNPV polyhedrin-plus (polh⁺) Bac-to-Bac system, designated as vBmBac(polh⁺)-enhanced green fluorescent protein (EGFP) and vBmBac(polh⁺)-LacZ, which can express the polyhedrin and foreign protein simultaneously. Light microscopy analysis showed that all viruses produced polyhedra of normal appearance. Green fluorescence can be apparently detected on the surface of the vBmBac(polh⁺)-EGFP polyhedra, but not the BmNPV polyhedra. Fluorescence analysis and anti-desiccation testing confirmed that EGFP was embedded in the polyhedra. As expected, the vBmBac(polh⁺)-LacZ polyhedra contained an amount of LacZ and had a higher β-galactosidase activity. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting were also performed to verify if the foreign proteins were immobilized into polyhedra. This study provides a new inspiration for efficient preservation of useful proteins and development of new pesticides with toxic proteins.  相似文献   

Heuristic algorithm for off-lattice protein folding problem   总被引：1，自引：0，他引：1  

Chen M  Huang WQ 《Journal of Zhejiang University. Science. B》2006,7(1):7-12

Enlightened by the law of interactions among objects in the physical world, we propose a heuristic algorithm for solving the three-dimensional （3D） off-lattice protein folding problem. Based on a physical model, the problem is converted from a nonlinear constraint-satisfied problem to an unconstrained optimization problem which can be solved by the well-known gradient method. To improve the efficiency of our algorithm, a strategy was introduced to generate initial configuration. Computational results showed that this algorithm could find states with lower energy than previously proposed ground states obtained by nPERM algorithm for all chains with length ranging from 13 to 55.  相似文献   

Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis     

Xu-fei Tan  Feng Chen  Shan-shan Wu  Yu Shi  Dong-cheng Liu  Zhi Chen 《Journal of Zhejiang University. Science. B》2010,11(3):221-226

Objective: To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis. Methods: Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel elec-trophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results. Results: In mice with Con A-induced hepatitis, expression levels of four proteins were increased: RIKEN, fructose bisphosphatase 1 (fbp1), ketohexokinase (khk), and Chain A of class pi glutathione S-transferase. Changes in fbp1 and khk were confirmed by qRT-PCR. Conclusion: Levels of two proteins, fbp1 and khk, are cleady up-regulated in mice with Con A-induced hepatitis.  相似文献   

腺苷酸活化蛋白激酶与运动性骨骼肌适应     

宋刚  严翊  周多奇 《成都体育学院学报》2006,32(4):86-90

通过文献检索方法对腺苷酸活化蛋白激酶参与运动引起骨骼肌适应的基因表达调节机制进行综述.从腺苷酸活化蛋白激酶相关生物知识、运动调节腺苷酸活化蛋白激酶激活和腺苷酸活化蛋白激酶参与运动训练与基因表达的可能作用三个方面进行探讨,为运动训练提供参考.  相似文献   

Preparation of protein samples for gel electrophoresis by sequential extraction   总被引：1，自引：0，他引：1  

钟伯雄  翁宏飚  方维焕 《浙江大学学报(A卷英文版)》2002,3(5):606-610

Since preparation and solubilization of protein samples are crucial factors in proteome research, the authors established a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm,Bombyx mori. Two kinds of protein samples were obtained from the body wall using the method. Between the two types of samples only about 15% proteins were identical; the majority were different, indicating that more species of proteins could be obtained with the sequential extraction method; which will be useful for preparation of protein samples for proteome study.  相似文献   

Protein Thiols and Butryrylcholinestrase in Saliva of Oral Cancer Patients     

Yousef Rezaei Chianeh  Rashmi Manjunath  Krishnananda Prabhu  Donald Fernandes  M. Vidyasagar  Asha Kamath 《Indian journal of clinical biochemistry : IJCB》2014,29(2):238-241

Oral squamous cell carcinoma is one of the most common malignancies recognized. Biomarkers which can predict presence of cancer and its progression can help in better management of these disorders. Over production of lipid peroxidation byproducts and disturbances in antioxidant defense system have been implicated in the pathogenesis of several diseases including oral cancer. Studies have shown a correlation of butyrylcholinesterase (BChE), with tumourigenesis, cell proliferation and cell differentiation. Earlier we have observed a significant elevation in plasma BChE and protein thiols in oral cancer patients which correlated well with stages of cancer. As it was not clear whether the above markers will be altered in saliva of oral cancer patients this study was undertaken. Institutional Ethics Committee gave permission to carry out this study. Total of 55 subjects comprising healthy controls (n = 30) and biopsy proven oral cancer patients (n = 25) consented to participate in this study. Salivary samples from cases were taken before any definitive treatment. Protein thiols and BChE were estimated in salivary samples using validated assay methods. Oral cancer patients had a significant increase in pre-treatment salivary BChE levels (p ≤ 0.001) and a significant decrease (p ≤ 0.001) in salivary thiols as compared to respective values in controls. Salivary protein thiols and BChE may have a role in pathophysiology of oral cancer. Saliva can be used as a potential non-invasive screening tool in oral cancer patients.  相似文献   

Correlation Between Proinflammatory Serum Markers: High Sensitivity C-Reactive Protein,Interleukin-6 with Disability Score in Acute Ischemic Stroke     

Bharosay A  Saxena K  Varma M  Bharosay VV  Pandey A 《Indian journal of clinical biochemistry : IJCB》2011,26(3):279-282

Stroke being the third leading cause of death and foremost cause of disability, if potential diagnostic utility of blood borne protein biomarkers in predicting acute stroke is established, it would be a substantial adjunct to computerized tomography and magnetic resonance imaging which have their own limitations. This study was done to correlate serum Interleukin 6, high sensitivity C reactive protein at the time of admission with neurological worsening assessed by NIHSS at the time of admission and 7 days after admission. 46 Patients admitted in neurology department SAIMS, Indore with first ever ischemic stroke within 72 h of onset were included in the study. All patients with history of stroke of more than 72 h onset, Infection & peripartum stroke were excluded from the study. Disability scoring was done by NIHSS and their serum samples assayed for hsCRP, IL6 by commercially available quantitative sandwich enzyme-linked immunoadsorbent assay kits. Serum samples of 50 control cases which included healthy volunteers and staff from SAIMS were also analyzed for hsCRP, IL6 for comparative study. A significant correlation was observed between NIHSS scoring and serum hsCRP and IL6 at the time of admission. Patients with initial high serum IL6 and hsCRP also showed significant clinical deterioration as assessed by NIHSS scoring 7 days after admission. Elevated hsCRP and IL6 within 72 h of admission strongly correlated with functional disability in study population in India and may serve as useful adjunct to CT Scan in emergency setting.  相似文献   

Combinatorial Determination of Ischemia Modified Albumin and Protein Carbonyl in the Diagnosis of NonST-Elevation Myocardial Infarction     

Kritsanee Maneewong  Titiporn Mekrungruangwong  Saowanee Luangaram  Tomon Thongsri  Sarawut Kumphune 《Indian journal of clinical biochemistry : IJCB》2011,26(4):389-395

Ischemia modified albumin (IMA) and Protein Carbonyl (PC) have known as proteins that are modified on the similar basis of oxidative stress induced protein modification and may have diagnostic potential in acute myocardial infarction. This study aims to evaluate the ability of using IMA and PC content to diagnose Non-ST elevation myocardial infarction (NSTEMI) and efficiency of combining these two markers. Serum from NSTEMI and healthy control were determined for serum IMA and PC content. The results showed that both of serum IMA level and PC content in NSTEMI was significantly higher than that of healthy controls. However, the PC content showed greater diagnostic performance than IMA. Combinatorial determination of serum IMA level with PC content level was enhanced test efficiency. In conclusion, our finding demonstrated that IMA and PC content can be used as a diagnostic marker for NSTEMI.  相似文献   

Genetic mapping of quantitative trait loci associated with β-amylase and limit dextrinase activities and β-glucan and protein fraction contents in barley     

Kang Wei  Da-wei Xue  You-zong Huang  Xiao-li Jin  Fei-bo Wu  Guo-ping Zhang 《Journal of Zhejiang University. Science. B》2009,10(11):839-846

High malting quality of barley (Hordeum vulgate L.) relies on many traits, such as β-amylase and limit dextrinase activities and β-glucan and protein fraction contents. In this study, interval mapping was utilized to detect quantitative trait loci (QTLs) affecting these malting quality parameters using a doubled haploid (DH) population from a cross of CM72 (six-rowed) by Gairdner (two-rowed) barley cultivars. A total of nine QTLs for eight traits were mapped to chromosomes 3H, 4H, 5H, and 7H. Five of the nine QTLs mapped to chromosome 3H, indicating a possible role of loci on chromosome 3H on malting quality. The phenotypic variation accounted by individual QTL ranged from 8.08% to 30.25%. The loci of QTLs for β-glucan and limit dex-trinase were identified on chromosomes 4H and 5H, respectively. QTL for hordeins was coincident with the region of silica eluate (SE) protein on 3HS, while QTLs for albumins, globulins, and total protein exhibited overlapping. One locus on chromosome 3H was found to be related to β-amylase, and two loci on chromosomes 5H and 7H were found to be associated with glutelins. The identification of these novel QTLs controlling malting quality may be useful for marker-assisted selection in improving barley malting quality.  相似文献   

花粉运动休闲饮料的研制     

孔瑾  赵功玲  宋照军  李富伟 《河南职业技术师范学院学报》2009,(1):66-69

以蛋白质提取率为主要指标,对花粉的发酵破壁提取和酶法破壁提取进行了研究．实验表明,当花粉加2．5倍水过胶体磨细化后,采用酶法提取工艺,在温度为45℃,pH为4．6的条件下,酶解30h,然后在60℃下浸泡10h,蛋白质提取率最高,效果最好．通过对花粉运动休闲饮料的调配试验,当花粉母液为20％、氯化钠为2％、蛋白糖为0．04％、柠檬酸为0．1％时,饮料的品质最佳．  相似文献