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11.
SNP（单核苷酸多态性）在生物基因组中具有数量多、分布广等特点,是目前广泛应用的第3代分子标记,在现代生物研究方面具有重要的应用价值。本研究综述了SNP的检测方法及其在农作物遗传育种中的应用。 相似文献
12.
目的：分析β2肾上腺素受体（ADRB2）基因位点＋46（A/G）单核苷酸多态性（SNP）与运动耐力相关性。方法：选取南方汉族人群,依据运动耐力素质的差异分成优秀运动耐力组（EEA）48人和普通组（SC）55人,采用片段长度差异等位基因特异性PCR的方法对受试者ADRB2基因多态性进行基因分型,通过χ2检验分析优秀运动耐力组和普通组之间的等位基因和基因型分布频率。结果：两组中ADRB2基因型的发生频率符合Hardy-Weinberg平衡;EEA组和SC组的SNP基因型和等位基因频率分布经χ2检验发现,两组该位点的基因型频率分布有显著差异（P〈0.05）,两组间等位基因频率分布也呈显著性差异（P〈0.05）。结论：数据表明在广东汉族人群中ADRB2基因A46G多态性与运动耐力相关。 相似文献
13.
Jing Li Xin-yue Liu Lin-jing Li Chong-ge You Lei Shi Shang-di Zhang Qian Liu Jun Wang Ze-jing Liu Ting-hong Lv 《Journal of Zhejiang University. Science. B》2015,16(7):632-639
A total of 64 patients with β-lactam allergy and 30 control subjects were enrolled in a case-control study. This study is aimed to analyze the relationship between β-lactam allergy and 10 single nucleotide polymorphisms (SNPs) in interleukin-10 (IL-10), IL-13, IL-4Rα, high-affinity immunoglobulin E-receptor β chain (FcεRIβ), interferon γ receptor 2 (IFNGR2), and CYP3A4, and within the Han Chinese population of Northwest China. Genotyping for the SNPs was conducted using the Sequenom MassARRAY®platform. SPSS 17.0 was employed to analyze the statistical data and SHEsis was used to perform the haplotype reconstruction and analyze linkage disequilibrium of SNPs of IL-10 and IL-13. The results showed that the genotype distribution of CYP3A4 rs2242480/CT differed significantly between case and control groups of males (P=0.022; odds ratio (OR)=0.167, 95% confidence interval (CI): 0.032–0.867). Further analysis showed that CCA, CCG, and TAA haplotypes of IL-10 had no significant correlation in patients with β-lactam allergy. The correlation between CCT and CAC haplotypes of IL-13 and β-lactam allergy needs to be further studied. The analysis did not reveal any differences in the distribution of others gene polymorphisms between cases and controls. 相似文献
14.
Jagdish Kaur Chahil Khamsigan Munretnam Nurulhafizah Samsudin Say Hean Lye Nikman Adli Nor Hashim Nurul Hanis Ramzi Sharmila Velapasamy Ler Lian Wee Livy Alex 《Indian journal of clinical biochemistry : IJCB》2015,30(2):134-139
Genome-wide association studies have discovered multiple single nucleotide polymorphisms (SNPs) associated with the risk of common diseases. The objective of this study was to demonstrate the replication of previously published SNPs that showed statistical significance for breast cancer in the Malaysian population. In this case–control study, 80 subjects for each group were recruited from various hospitals in Malaysia. A total of 768 SNPs were genotyped and analyzed to distinguish risk and protective alleles. A total of three SNPs were found to be associated with increased risk of breast cancer while six SNPs showed protective effect. All nine were statistically significant SNPs (p ≤ 0.01), five SNPs from previous studies were successfully replicated in our study. Significant modifiable (diet) and non-modifiable (family history of breast cancer in first degree relative) risk factors were also observed. We identified nine SNPs from this study to be either conferring susceptibility or protection to breast cancer which may serve as potential markers in risk prediction.
Electronic supplementary material
The online version of this article (doi:10.1007/s12291-013-0414-0) contains supplementary material, which is available to authorized users. 相似文献15.
16.
王骁力 《南阳师范学院学报》2007,6(3):1-4
讨论关于单体型的无间隙的最小单核苷酸多态性位点的移去问题.通过分析其对应图模型的性质讨论问题等价形式;证明求解该问题等价于求对应图的最大独立集与独立数;给出求最大独立集与独立数的算法,从而得到此问题的有效的多项式时间算法. 相似文献
17.
P. K. Manchanda H. K. Bid B. R. Achyut B. Mittal N Srivastava R. D. Mittal 《Indian journal of clinical biochemistry : IJCB》2007,22(1):61-64
The main adverse consequences of excess bodyweight are cardiovascular disease, type II diabetes, and several cancers, IL-1Ra
serum concentration has been reported earlier to increase in human obesity and it is therefore assumed that the polymorphism
of IL-1Ra may influence cytokine production. We designed this study to investigate whether the IL-1Ra polymorphism was associated
with obesity. A total number of 103 individuals; 19 lean (BMI<25 Kg/m2), 51 overweight (BMI 25–29.9 Kg/m2) and 33 obese (BMI≥30.0 Kg/m2) were enrolled in this study. Genotyping was performed using a polymerase chain reaction PCR amplification of the intron-2
fragment harboring a variable number of tandem repeat (VNTR) nucleotide sequences 86 pb of tandem repeat. The PCR products
were separated on 2% agarose gel. Statistical analysis was performed using SPSS software (version 11.5). We found no significant
difference in genotype and allele frequencies between the three groups; lean vs. overweight and lean vs. obese (p=0.323; 0.202;
0.123 and 0.068 resp). However, an increased risk for obesity had a propensity to be higher in those having genotype II/II.
This genotype has been reported to be a ‘high producer’ of IL-1Ra. Although no statistically significant relationship between
IL-1Ra polymorphism and BMI was observed, however, a trend towards an increase of allele*II in overweight and obese group was observed. This may suggest that IL-1Ra appears to be induced by inflammatory stimuli
as well as obesity-associated factors. This is relatively a pilot study: but nevertheless, may assist in identifying the pathophysiological
cause for obesity. 相似文献
18.
Emad F. Eskander Ahmed A. Abd-Rabou Mervat S. Mohamed Shaymaa M. M. Yahya Olfat G. Shaker 《Indian journal of clinical biochemistry : IJCB》2012,27(4):344-350
The main objective of the current study is to examine the role of the statistical relation between BCL2 gene (Ala43Thr) single nucleotide polymorphism and growth hormone (GH1) levels in Egyptian HCV genotype-4 patients before and after treatment with pegylated interferon plus ribavirin. Eighty patients with HCV genotype-4 and 40 healthy volunteers as controls were enrolled in the prospective study. Gene polymorphism of BCL2 (Ala43Thr) using PCR-RFLP technique and GH1 concentrations using ELISA procedure were measured for all patients and controls. The present study resulted that Responder HCV genotype-4 Patients, with BCL2 43Ala genotype, have high significant increase in pre-treatment GH1 levels (>1 ng/ml); which represent normal levels, as compared to non-responders pre-treatment GH1 levels (<1 ng/ml); which represent low concentrations. We concluded that HCV genotype-4 patients who have normal GH1 concentrations and BCL-2 43Ala genotype can successfully achieve response to interferon based therapy. 相似文献
19.
以＂新乡青＂萝卜为试材,研究了不同浓度外源水杨酸、钙和一氧化氮处理对高、低温胁迫下萝卜种子发芽的影响。结果表明,在35℃高温胁迫下,较低浓度的水杨酸和硝普钠处理可促进萝卜种子萌发,高浓度的水杨酸和硝普钠对种子萌发有抑制作用,水杨酸和硝普钠最佳处理浓度分别为0.01mmol.L-1和0.1mmol.L-1;不同浓度的外源钙处理对萝卜种子萌发均起抑制作用。在10℃低温胁迫下,低浓度的水杨酸和外源钙能促进种子萌发,浓度分别以0.005mmol.L-1和5mmol.L-1效果最佳,高浓度的水杨酸和外源钙对种子萌发有抑制作用;硝普钠各浓度处理对萝卜种子的萌发均具有抑制作用。 相似文献
20.
BackgroundThis study aimed to explore genetic polymorphisms of the CCKAR gene and their relationship with the growth and development of Qinchuan cattle which could be used as molecular markers for the improvement of the breeding of Qinchuan cattle.ResultsHere, we have identified seven single nucleotide polymorphisms (SNPs) at loci g. 1463 C>G; g. 1532 T>A; g. 1570 G>A; g. 1594 C>A; g. 1640 T>C; g. 1677 G>C; and g. 1735 C>T in the coding region of the bovine CCKAR gene. The frequencies identified on allelic and genotypic characteristics have shown that all seven SNPs diverged from the Hardy-Weinberg-Equilibrium. The SNP2, SNP3, SNP6 and SNP7 had the lowest polymorphism information content values, and remaining SNPs were found to be moderate (0.25 < PIC < 0.50). The genotype CG in SNP1 at loci g.1463 C>G had the greatest association with WH, HW, CD and CCF, while the genotype TA at the very same loci was associated with BFT, ULA and IMF content in Qinchuan cattle. The CCKAR gene expression level in adipose tissue, small intestine, liver and skeleton muscle was found to be higher, whereas, the expression level of mRNA in organs of other digestive system including reticulum, abomasum and omasum was moderate. Some expression of CCKAR mRNA was found in the large intestine, kidney and rumen.ConclusionsIn summary, our finding suggested that the CCKAR gene could be used as a potential candidate for the improvement of carcass quality and body measurements of Qinchuan cattle.How to citeNurgulsim K, Raza SHA, Khan R, et al. Identification of genetic variants the CCKAR gene and based on body measurement and carcass quality characteristics in Qinchuan beef cattle (Bos taurus). Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.02.001 相似文献