《大广益会玉篇》古今字初探     

贾中锋  刘力国 《黄河科技大学学报》2007,9(1):86-88

在《大广益会玉篇》中，古字和今字都作字头，并用特定的术语、特定的方式来标示。从形音义来分析。古字和令字有7种关系。本书中的令字是根据当时的用字情况确定的。古今字反映了4种用字现象，反映了文字演变的3个特点。  相似文献   

Preparation and in vitro studies of microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2     

Jiang Qiang  Zhang Su-zhan  Peng Jia-ping  Wang Xu-lin 《浙江大学学报(A卷英文版)》2005,6(9):859-864

Objective: To prepare microencapsulated cells releasing human tissue inhibitor of metalloproteinase-2 (TIMP-2), and investigate their biological characteristics in vitro. Methods: Chinese hamster ovary (CHO) cells were stably transfected with a human TIMP-2 expression vector, encapsulated in barium alginate microcapsules and cultured in vitro. Morphological appearance of the microcapsules was observed under a light microscope. Cell viability was assessed using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Enzyme linked immunosorbent assay (ELISA) and reverse zymography were used to confirm the release of biologically active TIMP-2 from the microcapsules. Cryopreservation study of the microencapsulated cells was carried out using dimethyl sulfoxide (DMSO) as preservative agent. Results: The microcapsules appeared like a sphere with diameter of 300–600 μm. The surface of the capsule wall was clearly smooth. The microencapsulated cells survived well and kept proliferating over the 6 weeks observed. No significant difference in TIMP-2 secretion was found between encapsulated and unencapsulated cells. Reverse zymography confirmed the bioactivity of MMP (matrix metalloproteinase) inhibition of TIMP-2. The cryopreservation process did not damage the microcapsule morphology nor the viability of the cells inside. Conclusion: Microencapsulated engineered CHO cells survive at least 6 weeks after preparation in vitro, and secrete bioactive TIMP-2 freely from the microcapsules.  相似文献   

题目：PGC-1α在CH1细胞中通过ATP和ROS调控细胞周期   总被引：2，自引：0，他引：2  

Xu-feng Fu  Kun Yao  Xing Du  Yan Li  Xiu-yu Yang  Min Yu  Mei-zhang Li  Qing-hua Cui 《Journal of Zhejiang University. Science. B》2016,17(2):136-146

  相似文献   

无脑儿心脏的组织学观察与比较     

付学清  梅盛平  万能章  雷天晓 《孝感职业技术学院学报》2006,9(2):80-81

目的:探讨无脑儿与正常胎儿心脏结构的差异,为畸形胎儿的心脏发育研究提供形态学方面的资料。方法:用H.E染色,通过光镜观察无脑儿及正常胎儿的心脏结构。结果:无脑儿心脏的蒲肯野细胞数量明显低于同龄正常胎儿。结论:无脑儿神经系统发育异常影响其心脏内蒲肯野细胞的发育。  相似文献   

《长生殿》的主题思想究竟是什么?——《长生殿·自序》解读     

刘宏 《阜阳师范学院学报(社会科学版)》2001,(1)

重新解读《长生殿·自序》,开辟一条寻绎《长生殿》主题思想的新途径,得出该剧主题思想的新结论,使《长生殿》主题思想的研究走出困境,提高了《长生殿》的思想价值。  相似文献   

Al3+对玉米根芽生长影响的研究     

田茂洁  杨艳  周平 《绵阳师范学院学报》2005,24(2):63-65

近年来，土壤中可溶性铝增加，对作物生长产生重要影响，本文研究了酸性条件下不同浓度Al^3＋对玉米根芽生长的影响。结果表明，Al对玉米根芽的生长有显著的抑制作用．且随着浓度的升高抑制作用显著加强，但时于根的生长Al^3＋浓度有一个相对促进其生长的浓度（0．5mg／L）；同时，高浓度的Al^3＋能诱导玉米根尖染色体发生多种类型畸变。  相似文献   

《说文解字》新证四则     

连劭名 《北京教育学院学报》2002,16(1):18-19

本文对<说文解字>中日、夙、外、应四个字的解释做了新的论证.日与实古代音义皆通,夙同肃,外的字义是"夕卜",龙是神,同时在古代哲学中代表中和之德.  相似文献   

COMPUTER SIMULATION FOR ELECTRICALLY DRIVEN MOTION OF FLUID     

崔铮 《东南大学学报》1989,(1)

In this paper,an equation system of electrohydrodynamics(EHD)based onthe fluid motion equations is discussed.Emphasis is put on the effects of electrical forceand surface tension upon the fluid motion.The Marker and Cell method is used to set up acomputational simulation program of electrically driven motion of fluid.With the help ofthe program,a cylindrical fluid under the influences of electrical field and surface tensionhas been calculated.The result is in good agreement with the experimental observation.  相似文献   

Review:DNA-damage response network at the crossroads of cell-cycle checkpoints, cellular senescence and apoptosis     

SCHMITT Estelle  PAQUET Claudie  BEAUCHEMIN Myriam  BERTRAND Richard 《Journal of Zhejiang University. Science. B》2007,(6)

Tissue homeostasis requires a carefully-orchestrated balance between cell proliferation, cellular senescence and cell death. Cells proliferate through a cell cycle that is tightly regulated by cyclin-dependent kinase activities. Cellular senescence is a safeguard program limiting the proliferative competence of cells in living organisms. Apoptosis eliminates unwanted cells by the coordinated activity of gene products that regulate and effect cell death. The intimate link between the cell cycle, cellular senes- cence, apoptosis regulation, cancer development and tumor responses to cancer treatment has become eminently apparent. Extensive research on tumor suppressor genes, oncogenes, the cell cycle and apoptosis regulatory genes has revealed how the DNA damage-sensing and -signaling pathways, referred to as the DNA-damage response network, are tied to cell proliferation, cell-cycle arrest, cellular senescence and apoptosis. DNA-damage responses are complex, involving “sensor” proteins that sense the damage, and transmit signals to “transducer” proteins, which, in turn, convey the signals to numerous “effector” proteins implicated in specific cellular pathways, including DNA repair mechanisms, cell-cycle checkpoints, cellular senescence and apoptosis. The Bcl-2 family of proteins stands among the most crucial regulators of apoptosis and performs vital functions in deciding whether a cell will live or die after cancer chemotherapy and irradiation. In addition, several studies have now revealed that members of the Bcl-2 family also interface with the cell cycle, DNA repair/recombination and cellular senescence, effects that are generally distinct from their function in apoptosis. In this review, we report progress in understanding the molecular networks that regulate cell-cycle checkpoints, cellular senescence and apoptosis after DNA damage, and discuss the influence of some Bcl-2 family members on cell-cycle checkpoint regulation.  相似文献   

茶叶细胞壁组分的分离及其稀土元素的分布与定位     

郭俊明  闵蔚宗  钟淑琳 《蒙自师范高等专科学校学报》1996,(2)

本研究结果表明,细胞壁确实是稀土元素沉积的主要地方之一;茶叶细胞壁经无水乙二胺萃取处理后,得到了三种组分,组分A经SephadexG—100上柱分离后,表明它至少含有两种大分子化合物。分子量较小的大分子物质的分子量约为5.0×10~3。氧化稀土总量在三种组分中的含量,按组分A、B和C顺序依次降低,即132.6μg/g、87.8μg/g和12.3μg/g。组分A和B中的稀土元素含量比细胞壁中的要高,表现出富集。本研究基本实现了从细胞水平和分子水平上探索稀土元素在植物体中的分布与定位。  相似文献