Functional characterization of an invertase inhibitor gene involved in sucrose metabolism in tomato fruit     

Ning ZHANG  Jing JIANG  Yan-li YANG  Zhi-he WANG 《Journal of Zhejiang University. Science. B》2015,16(10):845-856

In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato.  相似文献   

CRP,but not TNF-α or IL-6, decreases after weight loss in patients with morbid obesity exposed to intensive weight reduction and balneological treatment     

Danuta Ro??  Przemys?aw Adamczyk  Joanna Boinska  Robert Szafkowski  Irena Ponikowska  Katarzyna Stankowska  Barbara Góralczyk  Barbara Ruszkowska-Ciastek 《Journal of Zhejiang University. Science. B》2015,16(5):404-411

  相似文献   

Cytotoxicity and effect of extraction methods on the chemical composition of essential oils of Moringa oleifera seeds     

Rowland Monday Ojo Kayode  Anthony Jide Afolayan 《Journal of Zhejiang University. Science. B》2015,16(8):680-689

Renewed interest in natural materials as food flavors and preservatives has led to the search for suitable essential oils. Moringa oleifera seed essential oil was extracted by solvent-free microwave and hydrodistillation. This study assessed its chemical constituents. Cytotoxicity of the oils was investigated using hatchability and lethality tests on brine shrimps. A total of 16 and 26 compounds were isolated from the hydrodistillation extraction (HDE) and solvent-free microwave extraction (SME) oils, respectively, which accounted for 97.515% and 97.816% of total identifiable constituents, respectively. At 24 h when the most eggs had hatched, values of the SME (56.7%) and HDE (60.0%) oils were significantly different (P<0.05) from those of sea water (63.3%) and chloramphenicol (15.0%). Larva lethality was different significantly (P<0.05) between HDE and SME oils at different concentrations and incubation periods. The median lethal concentration (LC₅₀) of the oils was >1000 mg/ml recommended as an index for non-toxicity, which gives the oil advantage over some antioxidant, antimicrobial, therapeutic, and preservative chemicals.  相似文献   

Microcalorimetry studies of the antimicrobial actions of Aconitum alkaloids     

Yan-bin Shi  Lian Liu  Wei Shao  Ting Wei  Gui-mei Lin 《Journal of Zhejiang University. Science. B》2015,16(8):690-695

The metabolic activity of organisms can be measured by recording the heat output using microcalorimetry. In this paper, the total alkaloids in the traditional Chinese medicine Radix Aconiti Lateralis were extracted and applied to Escherichia coli and Staphylococcus aureus. The effect of alkaloids on bacteria growth was studied by microcalorimetry. The power-time curves were plotted with a thermal activity monitor (TAM) air isothermal microcalorimeter and parameters such as growth rate constant (μ), peak-time (T_m), inhibitory ratio (I), and enhancement ratio (E) were calculated. The relationships between the concentration of Aconitum alkaloids and μ of E. coli or S. aureus were discussed. The results showed that Aconitum alkaloids had little effect on E. coli and had a potentially inhibitory effect on the growth of S. aureus.  相似文献   

Using Six Sigma and Lean in the Library     

Elizabeth Nelson 《College & Undergraduate Libraries》2015,22(3-4):312-324

Businesses have long relied on a variety of strategies to improve quality, efficiency, and service. Some ideas that have persisted over time are Six Sigma and Lean. While Six Sigma, originally intended for manufacturing, focuses on quality and Lean mainly addresses waste, many of the tools and principles involved can also apply to libraries. This article will review the literature on Lean and Six Sigma as they are used in business and then as they have been used in libraries. The resulting discussion will cover how the principles can be applied to better connect with stakeholders by mapping processes and discovering what is value added (VA) versus nonvalue added (NVA), anticipating failure modes using the failure mode and effects analysis (FMEA) framework, and implementing control plans to maintain quality processes and service.  相似文献   

What Makes Access Services Staff Happy? A Job Satisfaction Survey     

Bethany Badgett Sewell  Charla Gilbert 《Journal Of Access Services》2015,12(3-4):47-74

This article reports the results of a survey that analyzed the job satisfaction of 361 library access services practitioners. Library access services staff were surveyed and asked to rate job satisfaction based on Maslow's Hierarchy of Needs and Herzberg's Two-Factor Theory. Results show that access services staff have opportunities for satisfaction based on higher-level needs. Personal fulfillment, job opportunities, and relationships with coworkers rated as the top three most important factors. There are also contributing factors that affect job satisfaction based on lower-level needs, such as salary. Age, education level, and administrative responsibilities also play a role in job satisfaction of access services staff. The survey results demonstrated that most employees are satisfied, while improvements are needed in certain areas to provide employees with a more fulfilling work environment.  相似文献   

Making It Fit: Reshaping Library Services and Spaces for Today's Students     

Paul Mosley  Jim Alderman  Lisandra R. Carmichael 《Journal Of Access Services》2015,12(3-4):91-103

University course assignments increasingly focus on group work and group projects to help students collaboratively discover more about their fields of study. The Thomas G. Carpenter Library realized the need to investigate means for reimagining itself to better accommodate today's researchers. The complex process of planning for the redesign of both services and space undertaken by the library is described and explained in this first of a planned series of articles.  相似文献   

The rhetorical construction of Popular Front France in the fascist Italian press during the ‘Paris, 1937’ international exhibition     

Francescomaria Evangelisti 《Media History》2015,21(4):481-492

This article will discuss propaganda in the press used by the Italian fascist regime to create and disseminate a negative image of France and socialism. The ‘Paris, 1937’ exhibition represented a pretext for the Italian press to attack the Popular Front on both policy and competence. Manipulating information, the propaganda fabricated an idea of ‘otherness’, presenting France as the chaotic antithesis to the orderly Italian regime. France was presented as a country full of drunks and idlers, as a nation descending into chaos due to the social economic reforms of the Blum government; images that were juxtaposed with a state thriving under fascist corporatism. With the use of archive files and press-clippings, this work intends to illustrate the construction of stereotypes to feed the fear of socialism in Italy by the fascist press.  相似文献   

Three ideas to advance our research base     

I. Diane Cooper 《Journal of the Medical Library Association》2015,103(4):169-170

  相似文献   

Starting the data conversation: informing data services at an academic health sciences library     

Kevin B. Read  Alisa Surkis  Catherine Larson  Aileen McCrillis  Alice Graff  Joey Nicholson  Juanchan Xu 《Journal of the Medical Library Association》2015,103(3):131-135

Objective

The research obtained information to plan data-related products and services.

Methods

Biomedical researchers in an academic medical center were selected using purposive sampling and interviewed using open-ended questions based on a literature review. Interviews were conducted until saturation was achieved.

Results

Interview responses informed library planners about researchers’ key data issues.

Conclusions

This approach proved valuable for planning data management products and services and raising library visibility among clients in the research data realm.  相似文献