超分子化合物[Ni(INAIP)(Him)(H2O)]·H2O的水热合成和晶体结构     

陈满生  邓奕芳  陈志敏  邝代治  杨颖群 《衡阳师范学院学报》2010,31(6)

由水热法合成了镍化合物[Ni(INAIP)(Him)(H2O)]·H2O,(1)(INAIP=5-异烟酰胺基异酞酸根),并对其进行了元素分析、IR及X-射线衍射法表征.晶体结构表明:配合物属于单斜晶系,P21/c空间群.配合物是由桥联配体5-异烟酰胺基异酞酸连接成二维(6,3)拓扑结构,该二维层被氢键拓展成三维超分子结构.  相似文献   

Science Letters:Proteomic analysis of differentially expressed proteins in mice with concanavalin A-induced hepatitis     

Xu-fei TAN  Feng CHEN  Shan-shan WU  Yu SHI  Dong-cheng LIU  Zhi CHEN 《Journal of Zhejiang University. Science. B》2010,(3)

Objective:To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis.Methods:Twenty-five mice were randomly divided into five groups:an untreated group,a control group injected with phosphate buffered saline (PBS),and groups with Con A-induced hepatitis evaluated at 1,3 and 6 h.Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identif...  相似文献   

Metachronous contralateral testicular and bilateral adrenal metastasis of chromophobe renal cell carcinoma: a case report and review of the literature     

Hai-yang Wu  Li-wei Xu  You-yun Zhang  Yan-lan Yu  Xin-de Li  Gong-hui Li 《Journal of Zhejiang University. Science. B》2010,11(5):386-389

Chromophobe renal cell carcinoma(ChRCC) metastatic to the testis has not,to the best of our knowledge,been reported in the literature.Nor have there been reports of delayed bilateral adrenal metastasis of ChRCC.Here we report a case of metachronous contralateral testicular and bilateral adrenal metastasis of ChRCC in a 70-year-old man who underwent right radical nephrectomy for RCC six years ago.He was admitted to the hospital because of left intrascrotal enlargement of two-month duration.Ultrasonography re...  相似文献   

Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways     

Feng Gao  Xin-yang Hu  Xiao-jie Xie  Qi-yuan Xu  Ya-ping Wang  Xian-bao Liu  Mei-xiang Xiang  Yong Sun  Jian-an Wang 《Journal of Zhejiang University. Science. B》2010,11(8):608-617

Mesenchymal stem cell(MSC)transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium,but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment.The aim of this study is to explore the cytoprotection of heat shock protein 90(Hsp90)against hypoxia and serum deprivation-induced apoptosis and the possible mechanisms in rat MSCs.Cell viability was determined by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Apoptosis was assessed by Hoechst 33258nuclear staining and flow cytometric analysis with annexin V/PI staining.The gene expression of Toll-like receptor-4(TLR-4)and V-erb-b2 erythroblastic leukemia viral oncogene homolog 2(ErbB2)was detected by real-time polymerase chain reaction(PCR).The protein levels of cleaved caspase-3,Bcl-2,Bcl-xL,Bax,total-ERK,phospho-ERK,totaI-Akt,phospho-Akt,and Hsp90 were detected by Western blot.The production of nitric oxide was measured by spectrophotometric assay.Hsp90 improves MSC viability and protects MSCs against apoptosis induced by serum deprivation and hypoxia.The protective role of Hsp90 not only elevates Bcl-2/Bax and Bcl-xL/Bax expression and attenuates cleaved caspase-3 expression via down-regulating membrane TLR-4 and ErbB2 receptors and then activating their downstream PI3K/Akt and ERK1/2 pathways,but also enhances the paracrine effect of MSCs.These findings demonstrated a novel and effective treatment strategy against MSC apoptosis in cell transplantation.  相似文献   

Neurochip based on light-addressable potentiometric sensor with wavelet transform de-noising     

Qing-jun Liu  Wei-wei Ye  Hui Yu  Ning Hu  Li-ping Du  Ping Wang 《Journal of Zhejiang University. Science. B》2010,11(5):323-331

Neurochip based on light-addressable potentiometric sensor(LAPS),whose sensing elements are excitable cells,can monitor electrophysiological properties of cultured neuron networks with cellular signals well analyzed.Here we report a kind of neurochip with rat pheochromocytoma(PC12) cells hybrid with LAPS and a method of de-noising signals based on wavelet transform.Cells were cultured on LAPS for several days to form networks,and we then used LAPS system to detect the extracellular potentials with signals d...  相似文献   

Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells     

Wei Wang  Li Wang  Xin-xiu Li  Xia Chen  Hai-yan Zhang  Yu He  Jing-jing Wang  Yong-yan Zhao  Bao-le Zhang  Yin-xue Xu 《Journal of Zhejiang University. Science. B》2010,11(9):719-727

Bone morphogenetic proteins (BMPs) play a critical role in the growth and steroidogenesis of granulosa cells (GCs). BMP signals act through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell types. The objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs. A strategy of RNA interference (RNAi)-mediated ‘gene silencing’ of Smad4, a core molecule mediating the intracellular BMP/Smad signal transduction pathways, was used to interrupt endogenous BMP/Smad signaling. Results indicate that Smad4-small interfering RNA (siRNA) caused specific inhibition of Smad4 mRNA and protein expression after transfection. Interrupted endogenous BMP/Smad signaling significantly inhibited growth, and induced apoptosis of porcine GCs, while decreasing estradiol production. In addition, interrupted BMP/Smad signaling significantly (P<0.05) changed the expression of Cyclin D2, CDK4, Bcl-2, and Cyp19a1. These findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.  相似文献   

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway     

Liu F  Zhong H  Liang JY  Fu P  Luo ZJ  Zhou L  Gou R  Huang J 《Journal of Zhejiang University. Science. B》2010,11(12):905-911

In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P0.05),while BMP-2 was not (P0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein.  相似文献   

Clinicopathological features of atypical lipomatous tumors of the laryngopharynx     

Shi HY  Wei LX  Wang HT  Sun L 《Journal of Zhejiang University. Science. B》2010,11(12):918-922

Atypical lipomatous tumor (ALT) of the laryngopharynx is rare. Here we report five cases to demonstrate their clinicopathological features. The patients were four males and one female, aged 41 to 69 years (median 53.6 years). All tumors (two in the hypopharynx and three in the larynx) presented as a slowly growing, painless mass. Symptoms included dysphagia (2/5), dysphonia (3/5), and the feeling of a foreign body in the throat (5/5). Tumors were well circumscribed or focally infiltrative, ranging from 2.0 to 5.0 cm (median, 3.4 cm) in size, and microscopically showed the typical features of lipoma-like ALT. Immunohistochemically, tumor cells were stained with S-100, vimentin, murine double minute 2 (MDM-2), and cyclin-dependent kinase 4 (CDK4). Two patients had local tumor recurrences at 6 and 14 months after initial surgery during follow-up. ALT of laryngopharynx is an indolent tumor. Immunohistochemical staining for MDM-2 and CDK4 is helpful in pathological diagnosis.  相似文献   

Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk   总被引：2，自引：0，他引：2  

Bin Huang  Yun Yin  Lei Lu  Hai Ding  Lin Wang  Ting Yu  Jia-jin Zhu  Xiao-dong Zheng  Yan-zhen Zhang 《Journal of Zhejiang University. Science. B》2010,11(10):812-818

A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive ELISA of CPFX had a concentration at 50% inhibition (IC₅₀) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%–84.60%, with coefficients of variation of less than 12.2%.  相似文献   

Case Report :Surgical intervention of severe post-ERCP-pancreatitis accompanied with duodenum perforation     

Zuo-bing CHEN  Zhong-yan LIANG  Yun ZHANG  Shao-yang ZHANG  Shu-sen ZHENG 《Journal of Zhejiang University. Science. B》2010,11(1)

Endoscopic retrograde cholangiopancreatography (ERCP) is a procedure widely used to diagnose and treat conditions of biliary or pancreatic ductal system. The post-ERCP severe acute pancreatitis (SAP) accompanied with duodenum perforation is rare but serious, remaining a challenge in clinic. In this study we report two such cases. Two Chinese women were treated for clinical suspicion of bile duct obstruction and underwent ERCP after admission. Both developed duodenum perforation and SAP after ERCP, and were ...  相似文献