技术创新专用性投资与治理机制   总被引：1，自引：0，他引：1  

苑泽明  严鸿雁 《科学学与科学技术管理》2009,30(5)

资产专用性理论把企业契约的不完全性作为逻辑分析的起点,把专用性资严投资当成逻辑的核心变量,把治理机制安排看成是逻辑的结果,整个理论在契约不完全一资产专用性一企业所有权安排这一分析框架中来运行.运用资产专用性理论分析框架,分析技术创新专用性投资不足的本质原因,提出技术生命周期不同阶段技术创新投资的专用性与其治理机制应该相互匹配.  相似文献   

Engineering G-quadruplex aptamer to modulate its binding specificity     

Long Li  Shujuan Xu  Xueyu Peng  Yuzhuo Ji  He Yan  Cheng Cui  Xiaowei Li  Xiaoshu Pan  Lu Yang  Liping Qiu  Jianhui Jiang  Weihong Tan 《国家科学评论(英文版)》2021,8(4)

The use of aptamers in bioanalytical and biomedical applications exploits their ability to recognize cell surface protein receptors. Targeted therapeutics and theranostics come to mind in this regard. However, protein receptors occur on both cancer and normal cells; as such, aptamers are now taxed with identifying high vs. low levels of protein expression. Inspired by the flexible template mechanism and elegant control of natural nucleic acid-based structures, we report an allosteric regulation strategy for constructing a structure-switching aptamer for enhanced target cell recognition by engineering aptamers with DNA intercalated motifs (i-motifs) responsive to the microenvironment, such as pH. Structure-switching sensitivity can be readily tuned by manipulating i-motif sequences. However, structure-switching sensitivity is difficult to estimate, making it equally difficult to effectively screen modified aptamers with the desired sensitivity. To address this problem, we selected a fluorescent probe capable of detecting G-quadruplex in complicated biological media.  相似文献