Pure total flavonoids from Citrus paradisi Macfadyen actmsynergistically with arsenic trioxide in inducingmapoptosis of Kasumi-1 leukemia cells in vitro     

Bo Wang  Sheng-yun Lin  Ying-ying Shen  Li-qiang Wu  Zhen-zhen Chen  Jing Li  Zhi Chen  Wen-bin Qian  Jian-ping Jiang 《Journal of Zhejiang University. Science. B》2015,16(7):580-585

To investigate the potential effects of pure total flavonoid compounds (PTFCs) from Citrus paradisi Macfadyen separately or combined with arsenic trioxide on the proliferation of human myeloid leukemia cells and the mechanisms underlying the action of PTFCs. The effects of PTFCs separately or combined with arsenic trioxide on the proliferation and apoptosis of leukemia cells were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), fluorescence microscopy, and flow cytometry. Their effects on the expression levels of apoptosis-related regulators were determined by Western blot assay. PTFCs combined with arsenic trioxide significantly inhibited the growth of Kasumi-1 cells, and apoptosis was confirmed by flow cytometry analysis. Hoechst 33258 staining showed more significant morphological changes and more apoptosis following the combined treatment. Western blots showed changes in the expression of genes for poly ADP-ribose polymerase (PARP), caspase 3/9, and P65. The results indicated that PTFCs separately or combined with arsenic trioxide inhibited proliferation of leukemia cells in vitro and induced their apoptosis by modulating the expression of apoptosis-related regulator genes.  相似文献   

现代结构特征的浅析与展望     

杜巍 《杨凌职业技术学院学报》2008,7(1):25-27

从现代结构的基本概念出发，阐明了结构的特征和前景展望，倡导发扬大胆创新与精心实践相结合的精神，推动现代结构和现代结构技术的加速发展。  相似文献   

组织学习与知识转移效用的实证研究   总被引：7，自引：1，他引：6       下载免费PDF全文

吴晓波  高忠仕  胡伊苹 《科学学研究》2009,27(1):101-110

 随着全球制造网络的形成,加强通过联盟合作向网络中的国际旗舰企业进行知识学习,对促进中国本土企业持续发展尤为重要。本文从采集的部分长三角地区制造业企业样本数据入手,运用多元线形回归分析了组织学习影响因素、调制因素（即联盟治理模式）对知识转移效用的影响作用,结果表明：国际旗舰企业知识属性、国际旗舰企业与中国本土企业之间关系、中国本土企业组织学习文化对知识转移效用有显著影响;中国本土企业学习能力与联盟治理模式、国际旗舰企业和中国本土企业之间关系与联盟治理模式形成的交互项对知识转移效用有显著影响,联盟治理模式起了调制作用。然后,对回归结果进行讨论,最后给出结论与展望。  相似文献   

女大学生身体自尊与体育学习项目兴趣及选择意向关系研究     

方小强  金新玉 《湖北体育科技》2009,28(6):651-653

通过随机抽取1 100名女大学生作为研究对象,研究女大学生身体自尊水平与体育学习项目兴趣和选择意向的关系。相关分析表明,女大学生身体自尊水平与体育学习项目兴趣与选择意向相关;女大学生身体自尊及各个下维维度是引起某些学习项目兴趣和选择意向不一致的重要因素。并提出相应身体自尊的干预措施,引导女大学生正确地进行选课。  相似文献   

农业科研机构科技产出绩效评价及其影响因素分析   总被引：3，自引：0，他引：3       下载免费PDF全文

申红芳  廖西元  胡慧英 《科研管理》2010,31(6):126-135

摘要：本文在对农业科研机构产出绩效的指标进行选择和综合评价的基础上，分析各单项产出及产出绩效的分布特征，并采用多元回归模型对农业科研机构产出绩效的影响因素进行实证分析。结果显示：我国农业科研机构产出绩效呈现明显的积聚特征；农业科研机构的产出受科研投入规模及结构的影响，人才是科技发展的第一资源；区域经济环境对科研产出具有显著影响，且科研产出距离生产越远，所受到区域经济环境的影响越小；科研产出与农业生产仍存在脱节。最后提出相应的对策建议。  相似文献   

Changes of CREB in rat hippocampus, prefrontal cortex and nucleus accumbens during three phases of morphine induced conditioned place preference in rats   总被引：10，自引：0，他引：10  

周连芳  朱永平 《Journal of Zhejiang University. Science. B》2006,7(2):107-113

  相似文献   

试论生态视域下的“轻负高质”     

祝新宇  刘贵华  周培植 《教育发展研究》2012,(2):53-56

"轻负高质"既是当前很多教育工作者所追求的一种理想境地,也是困扰教育界的难题。究其原因,"轻负高质"不是一个简单增减学业负担的问题,而是涉及教育活动的方方面面,甚至与整个教育环境中的各个因素都有着千丝万缕的联系。因而,仅仅依靠机械的线性思维很难厘清"轻负高质"所面临的千头万绪,必须要凭借一种有机的非线性思维来认识"轻负高质"。为了能从理论上对"轻负高质"进行科学的辨析,笔者尝试剖析生态视域下"轻负高质"的理念、关系及策略,以生态思维破解教育的"轻负高质"难题。  相似文献   

浙江省LED照明产业发展现状及其对策     

颜鹰  翁茂源  蒋建平 《科技管理研究》2012,32(9):158-161

分析浙江省LED照明产业发展概况及传统节能光源与LED照明竞争调研数据,得出产业发展特点。通过产品市场份额预测分析浙江省内LED照明产业发展趋势,针对LED上游投资风险,企业健康发展主要瓶颈,企业技术创新体系等问题,提出以投资发展中、下游性价比高、配套好的产业为主,增强产业发展动力,提升产业核心竞争力等三点建议。  相似文献   

浙江科技成果转化存在的问题及对策研究     

李明珍 《科技管理研究》2012,(15):34-37

通过对目前浙江科技成果转化过程中存在的问题进行分析,提出促进浙江省科技成果转化的对策和建议。  相似文献   

Protective effects of Salvia miltiorrhizae on the hearts of rats with severe acute pancreatits or obstructive jaundice     

Xi-ping Zhang  Guang-hua Feng  Jie Zhang  Yang Cai  Hua Tian  Xiao-feng Zhang  Yi-feng Zhou  Zhi-wei Wang  Ke-yi Wang 《Journal of Zhejiang University. Science. B》2009,10(3):193-202

Objective  To investigate the therapeutic effects and mechanisms of Salvia miltiorrhizae (Danshen) in the treatment of severe acute pancreatitis (SAP)- or obstructive jaundice (OJ)-induced heart injury. Methods  A total of 288 rats were used for SAP- (n=108) and OJ-associated (n=180) experiments. The rats were randomly divided into sham-operated, model control, and Salvia miltiorrhizae-treated groups. According to the difference of time points after operation, SAP rats in each group were subdivided into 3, 6 and 12 h subgroups (n=12), whereas OJ rats were subdivided into 7, 14, 21, and 28 d subgroups (n=15). At the corresponding time points after operation, the mortality rates of the rats, the contents of endotoxin and phospholipase A₂ (PLA₂) in blood, and pathological changes of the hearts were investigated. Results  The numbers of dead SAP and OJ rats in the treated groups declined as compared with those in the model control group, but not significantly (P>0.05). The contents of endotoxin (at 6 and 12 h in SAP rats and on 7, 14, 21, and 28 d in OJ rats, respectively) and PLA₂ (at 6 and 12 h in SAP rats and on 28 d in OJ rats, respectively) in the treated group were significantly lower than those in the model control group (P<0.01 and P<0.001, respectively). Besides, myocardial pathological injuries were mitigated in SAP and OJ rats. Conclusion  In this study, we found that Salvia miltiorrhizae improved myocardial pathological changes, reduced the content of PLA₂ in blood, and decreased the mortality rates of SAP and OJ rats, exerting protective effects on the hearts of the rats. Projected supported by the Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province (Nos. 2003C130 and 2004C142), the Grave Foundation Project for Technological and Development of Hangzhou City (No. 2003123B19), and the Intensive Foundation Project for Technology of Hangzhou City (No. 2004Z006), China  相似文献