Genetic Polymorphism of Angiotensin II Type 1 Receptors and Their Effect on the Clinical Outcome of Captopril Treatment in Arab Iraqi Patients with Acute Coronary Syndrome (Mid Euphrates)     

Fadhaa A. Ghafil  Bassim I. Mohammad  Hussain S. Al-Janabi  Najah R. Hadi  Hayder A. Al-Aubaidy 《Indian journal of clinical biochemistry : IJCB》2021,36(1):81

Genetic variation in the angiotensin II type 1 receptor (AT1R) has an important effect on the outcome of acute coronary syndrome (ACS) initiated treatment with captopril. This study aims to investigate the impact of genetic polymorphism of AT1R (rs5186 and rs275651) on the ACS outcome in Iraqi patients treated with captopril. A total of 250 Iraqi individuals with ACS were included in this case—control study and they were divided into two study groups; Study group 1 included 125 participants who were prescribed captopril, 25 mg twice daily and study group 2 included 125 participants who received no captopril as part of their ACS treatment (control study). The AT1R gene (rs5186) CC genotype was found to be associated with ST-elevation myocardial infarction (STEMI) (Odd’s ratio (O.R) = 1.2, P = 0.7), while AC was associated with Non-ST-elevation myocardial infarction (NSTEMI) and unstable angina (UA) (O.R = 1.2, P = 0.8). AC genotype is more prone to have Percutaneous coronary intervention (PCI) after ACS attack (O.R = 1.2, P = 0.6). CC genotype had a risk to get less improvement (O.R = 1.6, P = 0.5), so might require higher doses of captopril during acute coronary insult. The AT1R gene (rs275651) AA genotype was associated with UA (O.R = 1.3, P = 0.9). AA and AT genotypes were more prone to have PCI after ACS attack (O.R = 3.9 P = 0.2, O.R = 3.5, P = 0.3 respectively) and thus requiring higher doses of captopril. We conclude that the AT1R rs5186, rs275651 genetic polymorphisms might partially affect the clinical outcome of ACS patients treated with captopril and might have captopril resistance which requires higher doses.  相似文献   

不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制研究     

李洁  杨静  赵丽丽 《武汉体育学院学报》2019,53(5):88-94

目的：探讨不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制。方法：将雄性Wistar大鼠50只随机均分为5组：安静对照组（C）、低负荷运动训练组（LT）、中等负荷运动训练组（MT）、高负荷运动训练组（HT）和极高负荷运动训练组（ST），每组10只。各运动组分别进行6周的跑台运动训练。训练方案结束后，取腓肠肌样本，提取线粒体，测定线粒体柠檬酸合成酶（CS）、异柠檬酸脱氢酶（ICD）和α-酮戊二酸脱氢酶（α-KGDHC）活性；线粒体Ga²⁺含量、胞浆NADH、NAD⁺、ATP和ADP含量，以及ICD mRNA转录水平。结果：（1）不同负荷运动训练组线粒体CS、ICD和α-KGDHC的活性均显著高于安静对照组（P < 0.01），且CS和ICD活性由高到低顺序均为：MT组 > HT组 > ST组 > LT组 > C组，α-KGDHC活性由高到低顺序为：HT组 > MT组 > ST组 > LT组 > C组。（2）不同负荷运动训练组线粒体Ca²⁺ 含量均显著高于安静对照组（P < 0.01），其含量由高到低顺序为：MT组 > HT组 > ST组 > LT组 > C组；胞浆NADH/NAD⁺和ATP/ADP的比值均显著低于安静对照组（P < 0.01），其比值由低到高顺序为：MT组 < ST组 < HT组 < LT组 < C组。（3）不同负荷运动训练组ICD mRNA转录水平均高于安静对照组（P < 0.01），其水平由高到低顺序为： MT组 > HT组 > ST组 > LT组 > C组。结论：低负荷、中等负荷、高负荷及极高负荷运动训练均可提高大鼠骨骼肌线粒体三羧酸循环功能，且中等负荷运动训练效果最佳。其机制与胞浆NADH/NAD⁺和ATP/ADP比值、线粒体摄钙能力及限速酶基因的表达有关。  相似文献   

Genome-wide analysis of microRNA156 and its targets,the genes encoding SQUAMOSA promoter-binding protein-like (SPL) transcription factors,in the grass family Poaceae     

Yue  Erkui  Tao  Hua  Xu  Jianhong 《Journal of Zhejiang University. Science. B》2021,22(5):366-382

  相似文献   

红色基因传承融入高校思想政治教育的路径     

白小利 《船舶职业教育》2021,9(1):51-53

红色基因是中华优秀传统文化的精神财富,是社会主义核心价值观的组成部分.高校思想政治理论课是落实高校立德树人根本任务的关键课程,也是传承红色基因的主渠道和主阵地.通过分析红色基因传承在高校思想政治教育工作中的价值,探究如何将红色基因传承与高校思政课有效融合,进一步提升育人效果.  相似文献   

幽门螺杆菌CagA蛋白与CTB融合基因的克隆及其植物表达载体的构建   总被引：2，自引：0，他引：2  

姜云水  方平楚  付亚萍  朱诚 《科技通报》2004,20(3):189-193

目的　构建能在水稻胚乳中特异表达的幽门螺杆菌细胞毒素相关蛋白(CagA)和霍乱毒素B亚单位(CTB)的植物表达载体.方法　采用高保真PCR技术分别从质粒pGEM CTB和幽门螺杆菌基因组DNA中扩增出CTB和cagA基因,然后用PCR方法直接融合CTB基因和cagA基因.又用PCR法改造了水稻Wx启动子,在其3′端融合加入Ω序列和Kozak序列.通过一系列亚克隆最终将两个融合基因和NOS终止子插入根癌农杆菌双元载体pCAMBI A1300的表达框架内.结果　PCR验证和测序分析证明,CTB和cagA融合基因以正确的方向插入到载体pCAMBI A1300中,并位于水稻Wx启动子后.结论　成功构建CTB和cagA融合基因的植物表达载体,为幽门螺杆菌口服疫苗的研究奠定基础.  相似文献   

芜菁花叶病毒分离物NBXC的CP和HC-Pro基因克隆与序列分析     

施曼玲 《科技通报》2007,23(1):41-45

从浙江宁波雪菜上获得病毒分离物NBXC,病毒提纯后,电镜下可观察到大量长约740 nm的线形粒子。间接ELISA检测结果证实NBXC是芜菁花叶病毒(turnip mosaic virus,TuMV)的分离物。利用IC-RT-PCR对病毒分离物NBXC的CP和HC-Pro基因进行PCR扩增,分别得到约0.8 kb和1.4 kb的两条条带,与预期的TuMV CP和HC-Pro基因大小吻合。扩增产物克隆后进行序列测定,CP基因序列长度为864个核苷酸,编码288个氨基酸;HC-Pro基因序列长度为1374个核苷酸,编码458个氨基酸。NBXC的CP和HC-Pro基因与已报道的TuMV其他分离物的核苷酸序列同源率分别为90.0%～98.3%和82.2%～96.9%,氨基酸同源率分别为95.8%～99.3%和95.6%～99.3%。从CP和HC-Pro基因核苷酸序列同源性来看,NBXC与TuMV芸薹属分离物有更近的亲缘关系,而与萝卜属分离物亲缘关系较远。  相似文献   

Adiponectin Gene Polymorphism and its Association with Type 2 Diabetes Mellitus     

Biswas D  Vettriselvi V  Choudhury J  Jothimalar R 《Indian journal of clinical biochemistry : IJCB》2011,26(2):172-177

Mutations in different regions of adiponectin gene have been reported to be associated with obesity, atherosclerosis and type 2 diabetes mellitus. The present study was aimed to investigate the association among SNP 45 T > G of adiponectin gene and type 2 diabetes in South Indian population. 75 clinically diagnosed case of type 2 diabetes were studied and compared with 75 apparently healthy controls. The genotype frequency of SNP45 T > G in exon 2 of adiponectin gene was determined by PCR based restriction enzyme analysis using the restriction enzyme SmaI. (recognition site: CCC↓GGG). Three kind of genotypes: wild type TT (470 bp), heterozygous type TG (470 bp, 336 bp, 134 bp) and homozygote mutant type GG (336 bp, 134 bp) were studied. A positive association has been found between SNP45 T > G and type 2 diabetes in the study population (P = 0.010, OR = 3.797, 95% CI = 1.312–10.983). Therefore, SNP45T > G in adiponectin gene may be one of the risk factors for type 2 diabetes.  相似文献   

p73 基因与p53 抑癌基因的比较   总被引：1，自引：0，他引：1       下载免费PDF全文

吴小明 《惠州学院学报》2003,23(6):56-62

p53 是一种肿瘤抑制基因,它的突变与50 %的人类肿瘤发生有关。p73 基因编码的蛋白质,无论在结构上,还是在功能及调节上均与p53 蛋白相似,且与肿瘤的发生密切相关,本文对此方面的研究结果进行了比较,并对p73 能否作为候选抑癌基因进行了讨论.  相似文献   

基因与人类健康     

刘石泉  汤青云  钟桐生 《湖南城市学院学报》2002,19(6):116-118

介绍了人类基因组计划、功能基因组学研究、转基因研究的现状及其在揭示生命奥秘、维护人类健康方面所起的作用  相似文献   

鸡γ-干扰素的克隆和序列分析     

谢昆  王传铭  林丽飞  王栋  李河 《蒙自师范高等专科学校学报》2007,5(2):10-13

根据GenBank发表的鸡γ-干扰素核苷酸序列,使用primer 5设计一对特异性引物,通过RT-PCR技术从ConA诱导培养的鸡脾脏淋巴细胞中克隆出鸡γ-干扰素基因并对其进行测序,测序结果表明,鸡γ-干扰素基因全长495bp,具有一个完整的开放阅读框,编码164个氨基酸,与国外发表的序列比较,两序列间同源性为100%.计算机软件对鸡γ-干扰素编码的氨基酸序列进行了抗原性分析,结果表明鸡γ-干扰素具有良好的免疫原性.  相似文献