Comparison of Haptic and Biometric Properties,Bacterial Load,and Student Perception of Fixative Solutions: Formaldehyde Versus Chilean Conservative Fixative Solution with and without Formaldehyde in Pig Kidneys     

Marianne Skopnik-Chicago  Katherine Poblete-Cordero  Natali Zamora  Roberto Bastías  Pablo A. Lizana 《Anatomical sciences education》2021,14(6):836-846

One of the most widely used solutions to fix and preserve organic tissues is formaldehyde, despite reservations regarding its toxicity and the fact that formaldehyde-embalmed bodies lose their original characteristics. Anatomy laboratories have been replacing formaldehyde with solutions that retain the characteristics of fresh tissue. For this purpose, alternative solutions with a very low concentration of formaldehyde or without any formaldehyde have been analyzed. The objective of this study was to compare biometry, coloration, haptic properties, and bacterial load on animal specimens (pig kidneys) embalmed with formaldehyde, and with Chilean Conservative Fixative Solution with and without formaldehyde (formaldehyde chCFS and formaldehyde-free chCFS). Also, the perception of health and biological science students toward specimens treated with different solutions was assessed. The results indicated that there were no significant differences in specimens’ retraction, or bacterial load. Students showed a preference for organs embalmed in formaldehyde chCFS and formaldehyde-free chCFS; indicating that with these treatments they could better visualize structures and that the prosections had greater flexibility and the colors were more similar to those of fresh tissue. Additionally, students recommended the material embalmed in formaldehyde chCFS and formaldehyde-free chCFS for anatomy learning. In contrast, students indicated that formaldehyde-fixation negatively affected their practical experience. In conclusion, embalming with formaldehyde chCFS or formaldehyde-free chCFS provides an advantageous practical experience over the use of formaldehyde and may be an alternative to replace the use of formaldehyde in anatomy laboratories.  相似文献   

Effects of periodic carbohydrate ingestion on endurance and cognitive performances during a 40-km cycling time-trial under normobaric hypoxia in well-trained triathletes     

Yi-Hung Liao  Toby Mündel  Yan-Ting Yang  Chen-Chan Wei  Shiow-Chwen Tsai 《Journal of sports sciences》2019,37(16):1805-1815

The purpose of this study was to examine CHO ingestion on a cognitive task using a field-simulated time-trial (TT) under hypoxia in well-trained triathletes. Ten male triathletes (age: 22.1 ± 1.1 years; VO₂max: 59.4 ± 1.4 ml/kg/min) participated in this double-blind/crossover/counter-balanced design study. Participants completed 3 TT trials: 1) normoxic placebo (NPLA; FiO₂ = 20.9%), 2) hypoxic placebo (HPLA; FiO₂ = 16.3%), and 3) hypoxic CHO (HCHO; 6% CHO provided as 2 ml/kg/15 min; FiO₂ = 16.3%). During the TT, physiological responses (SpO₂, HR, RPE, and blood glucose/lactate), cognitive performance, and cerebral haemodynamics were measured. Hypoxia reduced TT performance by ~3.5–4% (p < 0.05), but CHO did not affect TT performance under hypoxia. For the cognitive task, CHO slightly preserved exercise-induced cognitive reaction speed but did not affect response accuracy during hypoxic exercise. However, CHO did not preserve the decreased Hb-Diff (cerebral blood flow, CBF) and increased HHb in the prefrontal lobe (p < 0.05) during hypoxic exercise, and CHO failed to preserve hypoxia-suppressed prefrontal CBF and tissue oxygen saturation. In conclusion, the present study demonstrates that CHO is effective in sustaining reaction speed for a cognitive task but not promoting TT performance during hypoxic exercise, which would be important for strategy-/decision-making when athletes compete at moderate high-altitude.  相似文献   

药用、食用、观赏用百合组培快繁研究进展   总被引：2，自引：0，他引：2  

阮瑶瑶  丁健  杨懋勋 《深圳职业技术学院学报》2011,10(1):71-75

百合是商品价值很高的植物品种,有药用、食用、观赏等用途．应用植物种苗组织培养快速繁殖技术,可在短期内生产大量优质的百合种苗．现阶段国内广泛开展了对百合的组织培养和植株再生途径的研究,百合的组织培养研究已较为成熟,多种外植体都可诱导分化出植株．本文从外植体和基本培养基的选择、激素对初代、增殖及生根培养的影响、组培苗移栽等方面,综述了近年来国内的药用、食用、观赏用百合组培快繁研究的现状．  相似文献   

生物医学工程:一个发展迅速值得关注的交叉学科——生物力学、组织工程学、生物材料与人工器官   总被引：4，自引：0，他引：4  

李恩中  曹河圻  杜生明 《中国科学基金》2006,20(3):153-158

生物医学工程学（Biomedical Engineering，BME）是包含多种技术并相互交叉融合的一门科学。它综合了生物学、医学与工程学的理论和方法，研究生命体的构造、功能、状态和变化，研究新材料、新技术、新仪器设备，用于防病、治病、保护人民健康和提高医学水平。涉及科学领域广泛，除生物学、医学外，还有电子学、微电子学、现代计算机技术、化学、高分子化学、力学、近代物理学、光学、射线学、精密机械和近代高技术，并在不断发展扩大，是各国争相大力发展的高技术之一。本文主要从生物力学、组织工程学、生物材料与人工器官等方面进行探讨。  相似文献   

藏成药七十味珍珠丸中矿物质元素在体内蓄积性水平研究   总被引：1，自引：0，他引：1  

李天才  索有瑞 《中国科学院研究生院学报》2003,20(3):348-352

名贵藏成药七十味珍珠丸是传统藏药的典型代表,疗效显著.其中矿物类药的大量运用为现代医学科学不可理喻,给传统藏医药学的健康发展带来了困惑。以金诃七十味珍珠丸为研究材料,以Wistar大白鼠为试验对象,用等离子体发射光谱(ICP AES)和氢化物原子吸收光谱(HAAS)等分析仪器,测定了对照组和给药组实验动物在连续 1 8周试验期间心、肝、肾等组织器官中的矿物质元素含量水平,阐述金诃七十味珍珠丸中矿物质元素在动物主要组织器官中的长期蓄积性及其对生物机体的毒性效应  相似文献   

人牙及骨组织中稀土分布状态的研究   总被引：1，自引：0，他引：1  

程发良  钟广涛 《科技通报》1994,10(6):380-382

测定了人体骨胳、牙齿中的稀土含量，考察了牙齿作为研究人体稀土代谢状况样品的可行性，研究了微量稀土元素在人牙、骨中的分布状态，提出了稀土在人体不同部位骨胳中分布的可能机制。  相似文献   

大花重瓣矮牵牛植株再生条件的研究     

江爱明  刘恒超  贾志强  王峰尖  刘冬梅 《郧阳师范高等专科学校学报》2011,31(3):88-89

矮牵牛在生产中常采用播种繁殖。但是种子大都是从国外进口的,价格昂贵,为了更快的获得大量种苗,通过对矮牵牛进行了快速繁殖方法的实验．主要采用组织培养技术繁殖．经半年多的研究和配方试验,掌握了矮牵牛的组织培养配方及技术,大大缩短了其繁殖周期．并保持其品种的稳定性．  相似文献   

植物组织培养中外植体发生褐变的原因及防止措施   总被引：2，自引：0，他引：2  

刘玲玲 《喀什师范学院学报》2011,32(3):44-46

通过对植物组织培养中外植体发生褐变的情况,分析了发生褐变的可能原因,并提出了相应的防止措施.  相似文献   

A stage-specific cell-manipulation platform for inducing endothelialization on demand     

Qilong Zhao  Juan Wang  Yunlong Wang  Huanqing Cui  Xuemin Du 《国家科学评论(英文版)》2020,7(3):629

Endothelialization is of great significance for vascular remodeling, as well as for the success of implanted vascular grafts/stents in cardiovascular disease treatment. However, desirable endothelialization on synthetic biomaterials remains greatly challenging owing to extreme difficulty in offering dynamic guidance on endothelial cell (EC) functions resembling the native extracellular matrix-mediated effects. Here, we demonstrate a bilayer platform with near-infrared-triggered transformable topographies, which can alter the geometries and functions of human ECs by tunable topographical cues in a remote-controlled manner, yet cause no damage to the cell viability. The migration and the adhesion/spreading of human ECs are respectively promoted by the temporary anisotropic and permanent isotropic topographies of the platform in turn, which appropriately meet the requirements of stage-specific EC manipulation for endothelialization. In addition to the potential of promoting the development of a new generation of vascular grafts/stents enabling rapid endothelialization, this stage-specific cell-manipulation platform also holds promise in various biomedical fields, since the needs for stepwise control over different cell functions are common in wound healing and various tissue-regeneration processes.  相似文献   

甜叶菊的组织培养     

崔广荣  何克勤  胡能兵  林平  张子学  王其斌  简善亮 《安徽科技学院学报》2011,25(5):23-28

以甜叶菊茎段为外植体、MS为基本培养基,研究了消毒剂70％酒精和0．1％升汞的不同时间处理组合的灭菌效果和对试管苗再生的影响,同时还探讨了不同种类、不同浓度植物生长调节剂对试管苗增殖、生根及移栽的影响。结果表明,70％酒精处理15-30s＋0．1％升汞处理4m较为适合茎段外植体灭菌,污染率低、苗再生率高;6-BA与NAA组合的试管苗增殖系数比KT、Ad与NAA组合要高,培养基中添加1．0mg／L6-BA＋0．1-0．2mg／LNAA植物生长调节剂组合适合试管苗增殖,增殖系数可达7．4;培养基中添加0．0-0．5mg／L的IBA或NAA均适合试管苗生根,生根的试管苗移栽成活率较高。  相似文献