不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制研究     

李洁  杨静  赵丽丽 《武汉体育学院学报》2019,53(5):88-94

目的：探讨不同负荷运动训练对大鼠骨骼肌线粒体三羧酸循环的影响及其机制。方法：将雄性Wistar大鼠50只随机均分为5组：安静对照组（C）、低负荷运动训练组（LT）、中等负荷运动训练组（MT）、高负荷运动训练组（HT）和极高负荷运动训练组（ST），每组10只。各运动组分别进行6周的跑台运动训练。训练方案结束后，取腓肠肌样本，提取线粒体，测定线粒体柠檬酸合成酶（CS）、异柠檬酸脱氢酶（ICD）和α-酮戊二酸脱氢酶（α-KGDHC）活性；线粒体Ga²⁺含量、胞浆NADH、NAD⁺、ATP和ADP含量，以及ICD mRNA转录水平。结果：（1）不同负荷运动训练组线粒体CS、ICD和α-KGDHC的活性均显著高于安静对照组（P < 0.01），且CS和ICD活性由高到低顺序均为：MT组 > HT组 > ST组 > LT组 > C组，α-KGDHC活性由高到低顺序为：HT组 > MT组 > ST组 > LT组 > C组。（2）不同负荷运动训练组线粒体Ca²⁺ 含量均显著高于安静对照组（P < 0.01），其含量由高到低顺序为：MT组 > HT组 > ST组 > LT组 > C组；胞浆NADH/NAD⁺和ATP/ADP的比值均显著低于安静对照组（P < 0.01），其比值由低到高顺序为：MT组 < ST组 < HT组 < LT组 < C组。（3）不同负荷运动训练组ICD mRNA转录水平均高于安静对照组（P < 0.01），其水平由高到低顺序为： MT组 > HT组 > ST组 > LT组 > C组。结论：低负荷、中等负荷、高负荷及极高负荷运动训练均可提高大鼠骨骼肌线粒体三羧酸循环功能，且中等负荷运动训练效果最佳。其机制与胞浆NADH/NAD⁺和ATP/ADP比值、线粒体摄钙能力及限速酶基因的表达有关。  相似文献   

短跑专项力量训练手段训练效应的实证研究——以拖重物跑为例     

王蓓  谢慧松  刘少飞 《武汉体育学院学报》2019,53(8):82-88

研究目的：探究拖重物跑训练手段对短跑运动员的身体素质、途中跑阶段的技术特征及下肢环节肌肉力量的影响，深入认识其对短跑途中跑技术和专项力量的作用机制。研究方法：对14名男子二级左右水平短跑运动员进行为期8周每周3次6%~10% BM负荷的拖重物跑训练，采用高速摄像分析法、等动肌力测试法分析运动员身体素质；支撑阶段髋、膝、踝关节运动学参数和下肢各环节肌肉力量实验前后的变化情况。结果：（1）实验后运动员30m、60m、立定跳远、立定三级跳远等身体素质及专项成绩显著提高；（2）步长、重心水平速度显著性提高，两大腿剪绞平均速度提高、单步时间减少；支撑阶段最小膝、踝角显著降低，角速度提高；（3）髋、膝关节伸/屈肌群PT/BW、AP除膝关节60°/sPT/BW值未见统计学意义，其余各角速度下PT/BW、AP均呈显著性差异，踝关节跖屈肌群各角速度下PT/BW值显著提高，跖屈肌群60°/s速度下AP提高，背屈肌群AP略降低。结论：适宜负荷的拖重物跑训练可显著改善短跑运动员运动素质、提高运动成绩；有利于提高髋关节剪绞-制动力量，使膝、踝关节处于低位超等长"屈蹬"状态；提高了髋、膝关节屈伸肌群快速主动收缩能力及踝关节跖屈肌群退让性快速收缩能力。  相似文献   

Reference power values for the jump squat exercise in elite athletes: A multicenter study     

Pedro L. Valenzuela  Michael McGuigan  Guillermo Sánchez-Martínez  Elaia Torrontegi  Javier Vázquez-Carrión  Zigor Montalvo 《Journal of sports sciences》2020,38(19):2273-2278

ABSTRACT

The present study aimed to provide reference values for lower-limb muscle power assessed during the incremental jump squat (JS) test in elite athletes (i.e., professional athletes competing at international level). We pooled data from all JS tests performed by elite athletes of different sports in two high-performance centres between 2015 and 2019, and computed reference values (i.e., terciles) for mean power (MP), mean propulsive power (MPP), and peak power (PP). Reference values were obtained from 684 elite athletes (458 male and 226 female) of 16 different sports (boxing, judo, karate, fencing, taekwondo, wrestling, basketball, soccer, futsal, handball, rugby union, badminton, tennis, long distance running, triathlon, and sprinting). Significant differences (p < 0.001) were found between male and female athletes for MP (7.47 ± 1.93 and 6.15 ± 1.68 W·Kg^?1, respectively), MPP (10.50 ± 2.75 and 8.63 ± 2.43 W·Kg^?1), and PP (23.64 ± 6.12 and 19.35 ± 5.49 W·Kg^?1). However, the velocity at which these power measures was attained seemed to be independent of sex (~0.95, 1.00 and 2.00 m·s^?1 for mean, mean propulsive, and peak velocity, respectively) and homogeneous across different sport disciplines (coefficient of variation <10%). These data can be used to classify athletes’ power capabilities, and the optimum velocity ranges provided here could be useful for training purposes.  相似文献   

Combined effects of exposure to hypoxia and cool on walking economy and muscle oxygenation profiles at tibialis anterior     

Masahiro Horiuchi  Yoko Handa-Kirihara  Daijiro Abe  Yoshiyuki Fukuoka 《Journal of sports sciences》2019,37(14):1638-1647

We investigated combined effects of ambient temperature (23°C or 13°C) and fraction of inspired oxygen (21%O₂ or 13%O₂) on energy cost of walking (C_w: J·kg^?1·km^?1) and economical speed (ES). Eighteen healthy young adults (11 males, seven females) walked at seven speeds from 0.67 to 1.67 m s^?1 (four min per stage). Environmental conditions were set; thermoneutral (N: 23°C) with normoxia (N: 21%O₂) = NN; 23°C (N) with hypoxia (H: 13%O₂) = NH; cool (C: 13°C) with 21%O₂ (N) = CN, and 13°C (C) with 13%O₂ (H) = CH. Muscle deoxygenation (HHb) and tissue O₂ saturation (StO₂) were measured at tibialis anterior. We found a significantly slower ES in NH (1.289 ± 0.091 m s^?1) and CH (1.275 ± 0.099 m s^?1) than in NN (1.334 ± 0.112 m s^?1) and CN (1.332 ± 0.104 m s^?1). Changes in HHb and StO₂ were related to the ES. These results suggested that the combined effects (exposure to hypoxia and cool) is nearly equal to exposure to hypoxia and cool individually. Specifically, acute moderate hypoxia slowed the ES by approx. 4%, but acute cool environment did not affect the ES. Further, HHb and StO₂ may partly account for an individual ES.  相似文献   

Physiological responses in different intensities of resistance exercise – Critical load and the effects of aging process     

Vivian Maria Arakelian  Cássia da Luz Goulart  Renata Gonçalves Mendes  Nuno Manoel de Sousa  Renata Trimer  Solange Guizilini 《Journal of sports sciences》2019,37(12):1420-1428

Aim was to identify critical load (CL) in young and elderly apparently healthy male cohorts. To contrast the metabolic, cardiovascular and perceptual responses on CL according to age. We evaluated 12 young (23 ± 3 years) and 10 elderly (70 ± 2 years) apparently healthy active males, who underwent: (1) 1 repetition maximum (1RM) test on a 45° Leg Press; (2) on different days, three high-intensity resistance exercise constant load tests (60%, 75% and 90% 1RM) until fatigue (Tlim). Absolute values of both the CL asymptote and curvature constant (kg) were significantly lower in elderly subjects (P < 0.05). In contrast, elderly subjects demonstrated a significantly higher number of repetitions at CL when compared with young subjects (P < 0.05). As expected, oxygen uptake (VO₂) and heart rate (HR) during maximal aerobic exercise testing were significantly reduced in older subjects. However, percent-predicted aerobic capacity were higher in older subjects (P < 0.05). In addition, blood lactate ([La^?]) corrected to Tlim and rating of perceived exertion values were greater in younger subjects at all intensities (P < 0.05). These findings, despite reduced force production in older subjects, endurance-related parameters are well preserved according to age-adjusted percent-predicted values in apparently healthy males.  相似文献   

上肢康复机器人主动康复训练控制策略     

刘鹏  葛小青  李宇  赵佳欣 《中国科学院大学学报》2019,36(4):570-576

近年来，脑卒中和交通事故造成上肢损伤的人数逐年上升，医疗领域对康复器械的需求越来越大。为满足实际需求，开发以气动人工肌肉为驱动器的4自由度（肩关节外收/内展（被动）、前驱/后伸、肘关节屈曲、腕关节屈曲）可穿戴上肢康复机器人。首先设计上肢康复机器人的结构，并采用气动人工肌肉作为肘关节与腕关节的驱动器。然后针对该上肢康复机器人，研究主动康复训练控制策略，设计两种控制策略。通过穿戴实验验证控制策略的有效性和实际应用价值。  相似文献   

Alternative assessment of knee joint muscle balance of soccer players through total work-based hamstring: quadriceps ratios     

Felipe Minozzo  Carlos Leonardo Figueiredo Machado  Eurico Nestor Wilhelm  Rafael Grazioli  Ronei Silveira Pinto 《European Journal of Sport Science》2018,18(10):1398-1404

Isokinetic hamstring-to-quadriceps (H:Q) ratios are frequently used to assess knee muscle strength imbalances and risk of injuries/re-injuries. The use of peak torque (PT) or total work (TW) to estimate joint stability may lead to different results because of the differences between these two neuromuscular variables. Thus, the current study aimed to compare the conventional and functional H:Q ratios calculated by PT and TW. Ninety-three male professional soccer players from Brazilian first division teams performed isokinetic concentric and eccentric contractions of the quadriceps and the hamstrings at 60°/s. Muscle strength balance was calculated using the conventional torque ratio (CTR) and conventional work ratio (CWR), functional torque ratio (FTR) and functional work ratio (FWR) were highly and moderately correlated between them (r?=?0.83 and r?=?0.73, respectively). The Wilcoxon statistical test revealed significant differences between CTR and CWR, as well as FTR and FWR (p?T-test demonstrated significant differences in mean CTR–CWR and FTR–FWR, whereas Bland–Altman plots showed non-consistent bias. In addition, the chi-square test demonstrated significant differences between players below the conventional reference values and functional reference values (p?相似文献   

Reliability and Validity of Finger Strength and Endurance Measurements in Rock Climbing     

Michail Lubomirov Michailov  Jiří Baláš  Stoyan Kolev Tanev  Hristo Stoyanov Andonov  Jan Kodejška  Lee Brown 《Research quarterly for exercise and sport》2018,89(2):246-254

Purpose: An advanced system for the assessment of climbing-specific performance was developed and used to: (a) investigate the effect of arm fixation (AF) on construct validity evidence and reliability of climbing-specific finger-strength measurement; (b) assess reliability of finger-strength and endurance measurements; and (c) evaluate the relationship between finger flexor all-out test scores and climbing ability. Methods: To determine the effect of AF, 22 male climbers performed 2 maximal strength and all-out tests with AF (shoulder and elbow flexed at 90°) and without AF (shoulder flexed at 180° and elbow fully extended). To determine reliability, 9 male climbers completed 2 maximal strength tests with and without AF and an all-out and intermittent test without AF. Results: The maximal strength test without AF more strongly determined climbing ability than the test with AF (r² = .48 and r² = .42 for sport climbing; r² = .66 and r² = .42 for bouldering, respectively). Force and time variables were highly reliable; the rate of force development and fatigue index had moderate and low reliability. The maximal strength test with AF provided slightly higher reliability than without AF (intraclass correlation coefficient [ICC] = 0.94, ICC = 0.88, respectively). However, smaller maximal forces were achieved during AF (484 ± 112 N) than without AF (546 ± 132 N). All-out test average force had sufficiently high reliability (ICC = 0.92) and a relationship to sport climbing (r² = .42) and bouldering ability (r² = .58). Conclusion: Finger strength and endurance measurements provided sufficient construct validity evidence and high reliability for time and force parameters. Arm fixation provides more reliable results; however, the position without AF is recommended as it is more related to climbing ability.  相似文献   

Effect of 6-weeks WBVT on the behaviour of the lower limb muscle fibres during vertical jumping     

Jacobo Ángel Rubio-Arias  Domingo Jesús Ramos-Campo  Paula Esteban  Fernando Martínez  José Fernando Jiménez 《Journal of sports sciences》2018,36(4):398-406

The purpose of the current study was to examine the effect of 6 weeks of whole body vibration training (WBVT) on body composition, muscle activity of the gastrocnemius and vastus lateralis, gastrocnemius muscle architecture (static and dynamic) and ground reaction forces (performance jump) during the take-off phase of a countermovement jump in young healthy adult males. A total of 33 men (23.31 ± 5.62 years) were randomly assigned to a whole body vibration group (experimental group, EG_WBVT: n = 17; 22.11 ± 4.97 years) or a control group (CG: n = 16; 24.5 ± 6.27 years). The total duration of the intervention phase (WBVT) was 6 weeks with a frequency of 3 sessions per week. Statistically significant differences were observed (P ≤ 0.05) between pre- and post-test in the power peak (Δ 1.91 W · kg^?1; P = 0.001), take-off velocity (0.1 cm · s^?1; P = 0.002) and jump height (Δ 0.4 cm; P = 0.002) for EG_WBVT. There were no statistically significant differences in any of the body composition and muscle architecture variables. Moreover, no significant differences were found between EG_WBVT and CG nor changes in muscle activity during take-off phase of the gastrocnemius and vastus lateralis pre- versus post-training. This study suggests that a 6-week WBVT programme with increasing intensity improves jump performance but does not alter muscle activity nor muscle architecture in healthy young men.  相似文献   

Protective effects of photobiomodulation against resistance exercise-induced muscle damage and inflammation in rats     

Helenita Antonia de Oliveira  Ednei Luiz Antonio  Flávio André Silva  Paulo de Tarso Camillo de Carvalho  Regiane Feliciano  Amanda Yoshizaki 《Journal of sports sciences》2018,36(20):2349-2357

We investigated whether low-level laser therapy (LLLT) prior to or post resistance exercise could attenuate muscle damage and inflammation. Female Wistar rats were assigned to non-LLLT or LLLT groups. An 830-nm DMC Laser Photon III was used to irradiate their hind legs with 2J, 4J, and 8J doses. Irradiations were performed prior to or post (4J) resistance exercise bouts. Resistance exercise consisted of four maximum load climbs. The load work during a resistance exercise bout was similar between Control (non-LLLT, 225 ± 10 g), 2J (215 ± 8 g), 4J (210 ± 9 g), and 8J (226 ± 9 g) groups. Prior LLLT did not induce climbing performance improvement, but exposure to 4J irradiation resulted in lower blood lactate levels post-exercise. The 4J dose decreased creatine kinase and lactic dehydrogenase levels post-exercise regardless of the time of application. Moreover, 4-J irradiation exposure significantly attenuated tumor necrosis factor alpha, interleukin-6, interleukin-1β, cytokine-induced neutrophil chemoattractant-1, and monocyte chemoattractant protein-1. There was minor macrophage muscle infiltration in 4J-exposed rats. These data indicate that LLLT prior to or post resistance exercise can reduce muscle damage and inflammation, resulting in muscle recovery improvement. We attempted to determine an ideal LLLT dose for suitable results, wherein 4J irradiation exposure showed a significant protective role.  相似文献