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1.
目的:构建2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)以及恶唑酮(oxazolone,OXZ)诱导的小鼠溃疡性结肠炎(ulcerative colitis,UC)模型。方法:120只昆明小鼠()随机分为4组(n=30)。Ⅰ组、Ⅱ组参照Morris及Walter的方法制备小鼠TNBS模型:Ⅰ组(TNBS溶剂对照组),50%乙醇0.1 m L灌肠;Ⅱ组(TNBS模型组),0.6%TNBS溶液0.1 m L灌肠;两组灌肠给药一次后在d 1、d 2、d 3、d 5、d 7每组处死6只。Ⅲ组、Ⅳ组参照Heller方法制备小鼠OXZ模型:Ⅲ组(OXZ溶剂对照组),皮肤涂抹100%乙醇0.1 m L,每天一次,连续2 d,d 7以50%乙醇0.1 m L灌肠;Ⅳ组(OXZ模型组),皮肤涂抹1%OXZ溶液(100%乙醇溶解)0.1 m L每天一次,连续2 d(致敏),d 7以0.5%OXZ(50%乙醇溶解)0.1 m L灌肠;两组灌肠给药一次后在d 1~d 5每天处死6只小鼠。观察Ⅰ~Ⅳ组小鼠疾病活动指数(disease activity index,DAI)、结肠组织大体损伤指数(colon macroscopic damage index,CMDI)和病理组织学评分(histopathological score,HPS),并检测小鼠结肠组织中髓过氧化物酶(myeloperoxidase,MPO)、白细胞介素-4(interleukin-4,IL-4)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的水平。结果:两种模型组小鼠DAI,CMDI和HPS均较对照组有明显改变;TNBS和OXZ诱导的结肠炎均可导致MPO明显上升,TNBS结肠炎TNF-α明显上升,OXZ结肠炎IL-4明显下降。结论:TNBS及OXZ均能诱导小鼠溃疡性结肠炎模型。两种模型各有特点,其中TNBS诱导的小鼠溃疡性结肠炎以辅助性T1(helper1,Th1)型炎症反应为主,OXZ诱导的小鼠溃疡性结肠炎以辅助性T2(helper2,Th2)型炎症反应为主。  相似文献   
2.
目的:探索腹腔注射丁酸盐对防止乙酸性结肠炎的疗效。创新点:首次对大鼠进行腹腔注射丁酸盐,通过与直肠灌注和口服比较,探索三种不同给药方式对预防乙酸性结肠炎的疗效差异。方法:以40只Wistar大鼠为实验对象,分组进行连续7天的腹腔注射、直肠灌注和口服100 mg/kg丁酸钠(SB),第8天进行乙酸(AA)直肠灌注,48小时后处死。记录实验大鼠的临床症状,包括体重减少、腹泻、便血等。对结肠切片进行组织病理学观察,最后对试验数据进行统计分析。结论:腹腔注射、直肠灌注和口服丁酸盐均能明显缓解大鼠乙酸性结肠炎的炎症,其中以腹腔注射疗效最佳。  相似文献   
3.
溃疡性结肠炎(ulcerative colitis,UC)是一种原因不明主要发生在结肠粘膜层的炎症性病变,以溃疡糜烂为主。其病因及发病机制目前尚无定论,但倾向性意见是UC与自身免疫紊乱有关,所涉及到的自身抗体主要归纳为两类,一类是抗结肠抗体(ACA),另一类是抗人中性粒细胞胞质抗体(ANCA)。本文就溃疡性结肠炎免疫学发病机理及血清自身抗体检测的临床应用加以阐述,以便为UC病因学和诊断学的研究提供客观依据。  相似文献   
4.
We hypothesized whether systemic administration of high-molecular-weight hyaluronic acid (HMW HA) could rescue trinitrobenzene sulfonic acid (TNBS)-induced colitis through Toll-like receptor 4 (TLR4) signal. C3H/HeN mice and C3H/HeJ mice were used. Mice were divided into four groups: control, 50% ethanol treatment group, TNBS treatment group, and TNBS plus HA treatment group. The weight changes, clinical scores, macroscopic scores, and histological scores were recorded. Cyclooxygenase 2 (Cox-2) and prostaglandin E2 (PGE2) expressions were measured both in colons and peritoneal macrophages from these mice. HA was a rescue therapy for the colitis induced by TNBS only in C3H/HeN mice. The clinical score, macroscopic score, and histological score were much lower in C3H/HeN mice receiving TNBS plus HA treatment. Cox-2 and PGE2 expressions only increased in C3H/HeN mice. These Cox-2 expressing cells were macrophages. HA can also promote the production of Cox-2 and PGE2 in peritoneal macrophages from C3H/HeN mice. Our data demonstrated that HMW HA can rescue TNBS-induced colitis through inducing Cox-2 and PGE2 expressions in a TLR4-dependent way. Macrophages may be the effector cells of HMW HA.  相似文献   
5.
溃疡性结肠炎(ulcerative colitis,UC)又称慢性非特异性溃疡性结肠炎,流行病学资料提示溃疡性结肠炎的发病率在国内外都有逐年增高的趋势,溃疡性结肠炎的研究越来越受到国际肠病学家的关注,所以建立接近于人类的稳定的溃疡性结肠炎动物模型尤为重要。本文综述了近几年常用的诱发溃疡性结肠炎动物模型的化学方法及评价,以期为基础与临床研究应用提供参考。  相似文献   
6.
Inflammatory bowel disease (IBD) is a heterogeneous group of chronic inflammatory disorders of the gastrointestinal tract with two main distinguishable entities, Crohn’s disease (CD) and ulcerative colitis (UC). IBD-unclassified (IBD-U) is a diagnosis that covers the “grey” zone of diagnostic uncertainty between UC and CD. Current diagnosis of IBD relies on the clinical, endoscopic, radiological, histological and biochemical features, but this approach has shortcomings especially in cases of overlapping symptoms of CD and UC. The need for a diagnostic tool that would improve the conventional methods in IBD diagnosis directed the search towards potential immunological markers, since an aberrant immune response against microbial or endogenous antigens in a genetically susceptible host seems to be implicated in IBD pathogenesis. The spectrum of antibodies to different microbial antigens and autoantibodies associated with IBD is rapidly expanding. Most of these antibodies are associated with CD like anti-glycan antibodies: anti-Saccharomices cerevisiae (ASCA) and the recently described anti-laminaribioside (ALCA), anti-chitobioside (ACCA), anti-mannobioside (AMCA), anti-laminarin (anti-L) and anti-chitin (anti-C) antibodies; in addition to other antibodies that target microbial antigens: anti-outer membrane porin C (anti-OmpC), anti-Cbir1 flagellin and anti-I2 antibody. Also, autoantibodies targeting the exocrine pancreas (PAB) were shown to be highly specific for CD. In contrast, UC has been associated with anti-neutrophil cytoplasmic autoantibodies (pANCA) and antibodies against goblet cells (GAB). Current evidence suggests that serologic panels of multiple antibodies are useful in differential diagnosis of CD versus UC and can be a valuable aid in stratifying patients according to disease phenotype and risk of complications.  相似文献   
7.
以水杨酸为原料,采用浓硝酸/冰醋酸作为硝化剂,合成5-硝基水杨酸;再用铁粉还原制得抗结肠炎药美沙拉嗪.合成路线简便,操作简单,设备腐蚀较小,反应条件温和,收率较高,适合工业化生产.  相似文献   
8.
康复新液联合思密达灌肠治疗34例溃疡性结肠炎疗效   总被引:1,自引:0,他引:1  
王娟  张可 《大连大学学报》2009,30(6):104-105
60例溃疡性结肠炎患者随机分为2组:治疗组34例,予0.9%氯化钠注射液20或50ml加康复新液20ml及思密达3g保留灌肠;对照组26例,予0.9%氯化钠注射液20或50ml加思密达3g保留灌肠,均2次/天.治疗1月后复查结肠镜,观察疗效。结果表明,治疗组总有效率97.1%,对照组总有效率73.1%%,两组有显著性差异(P〈0.05)。康复新液联合思密达灌肠治疗溃疡性结肠炎临床效果显著。  相似文献   
9.
改进保留灌肠方法治疗结肠炎的临床观察   总被引:1,自引:0,他引:1  
目的:进一步提高药物保留灌肠治疗结肠炎的效果。方法:通过对90例结肠炎病人1350次保留灌肠,其中传统保留灌肠法180次,改进保留灌肠法1100余次。两种方法比较发现,改进的保留灌肠法对病人刺激小,基本无出血现象,药物在肠道保留、作用时间长,治疗效果明显。  相似文献   
10.

Objective  

Inflammatory bowel diseases (IBDs) are idiopathic, chronic, and inflammatory intestinal disorders. The two main types, ulcerative colitis (UC) and Crohn’s disease (CD), sometimes mimic each other and are not readily distinguishable. The purpose of this study was to present a series of hospitalized cases, which could not initially be classified as a subtype of IBD, and to try to note roles of the terms indeterminate colitis (IC) and inflammatory bowel disease unclassified (IBDU) when such a dilemma arises.  相似文献   
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