Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L.and Oryza meyeriana L.     

严成其  钱凯先  薛刚平  吴忠长  陈跃磊  颜秋生  张雪琴  吴平 《浙江大学学报(A卷英文版)》2004,5(10)

Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice bfeeding program for the improvement of bacterial blight resistance.  相似文献   

2型糖尿病大鼠基因表达变化的研究     

张曙云  沈征  钱凯先 《科技通报》2007,23(3):368-371

研究2型糖尿病大鼠和正常大鼠肾脏组织差异表达基因,筛选与糖尿病相关的基因。应用基因芯片技术对糖尿病和正常大鼠的肾脏组织的mRNA进行检测。在4096条目的基因中共发现差异基因41条（其中5个基因上调,36个基因下调）,全部在GeneBank中登录。2型糖尿病的发生、发展涉及多基因改变,用基因芯片在寻找新的2型糖尿病相关基因方面有极大的应用前景,对于糖尿病的诊断,治疗和预防具有一定的临床应用前景。  相似文献   

Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.   总被引：2，自引：0，他引：2  

严成其  钱凯先  薛刚平  吴忠长  陈跃磊  颜秋生  张雪琴  吴平 《Journal of Zhejiang University. Science. B》2004,(10)

Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.  相似文献