Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity   总被引：1，自引：0，他引：1  

韩明勇  郑树  于金明  彭佳萍  郭其森  王家林 《Journal of Zhejiang University. Science. B》2004,(4)

INTRODUCTIONInterleukin-18(IL-18),originallydescribedasinterferon(IFN)-g-inducingfactor,isan18.3-kDaproinflammatorycytokineproducedbyactivatedmacrophagesanddendriticcells,andplaysanim-portantroleintheTh1response,primarilybasedonitsabilitytoinduceIFN-gproductioninTcellsandNKcells.IL-18inducesproliferationofactivatedTcells,secretionofseveralcytokines,andpartici-patesinbothinnateandacquiredimmunity.TheroleofIL-18inantitumorimmunitywassuggestedinmanystudies.Genetransferofcytokinesisani…  相似文献   

浅议中国动画产业人才培养     

韩明勇 《中国轻工教育》2011,(2):25-26,65

近些年,动画产业在我国发展迅猛,动画产业的人才需求量也急剧上升,但我国动画教育却存在着许多与产业发展不相适应的问题,本文在对我国动画教育现状的分析上,针对动画人才培养提出了一些建议。  相似文献   

Study on interleukin-18 gene transfer into human breast cancer cells to prevent tumorigenicity     

韩明勇  郑树  于金明  彭佳萍  郭其森  王家林 《浙江大学学报(A卷英文版)》2004,5(4):472-476

To study the effect of interleukin-18 gene transfection on the tumorigenesis of breast cancer cell line Bacp37, human breast cancer cell line Bcap37 were transfected with Lipofectamine and selected by G418. The biological expression of rhIL-18 was tested by RT-PCR and ELISA method; nude mice were injected with Bcap37 cell with or without the hIL-18 gene. The hIL-18 cDNA was successfully integrated into Bcap37 cell; 126.3±4.5 pg hIL-18 secreted by one million transduced cells in 24 hours. Nude mice injected with IL-18 gene engineered Bcap37 cell had no tumor growth. These findings indicated that human breast cancer cells were successfully modified by the gene of IL-18 cytokine; the IL-18 gene engineered Bcap37 cells secreted hIL-18 and lost their tumorigenicity. The Bcap37 cells transduced with IL-18 gene may be used as breast cancer vaccine.  相似文献   

高校“艺术硕士专业学位教育”探索与实践     

韩明勇 《教育教学论坛》2010,(9)

艺术硕士专业学位教育是我国艺术学学位与研究生教育改革的重要内容,旨在培养高层次、应用型艺术专门人才。探索和研究艺术硕士专业学位教育的教学实施方案和教学具体措施,对艺术硕士专业学位教育"确保质量,突出特色"很有裨益。  相似文献