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INTRODUCTION It is known that human embryo fibroblast cellsare permissive for cytomegalovirus (CMV) repli-cation in vitro. Data have shown that CMV infec-tion causes a rapid, progressive disruption of thehost cell cytoskeleton (CSK); and that the disrup-tion correlates with actin depolymerization. Someexperts used whole-mount (3D) electron micros-copy to analyze the CSK of uninfected and CMV-infected human lung fibroblast cells; and foundwithin 2 min of CMV infection, localiz… 相似文献
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作文教学是语文教学的重要组成部分,学生的作文水平反映了其语文素养。许多学生在习作时总是抓耳挠腮,一脸的无奈,既使硬着头皮去写,写出来的作文也是无血无肉,缺少真情实感。面对如此现状,作为一名任教多年的教师,笔者不禁陷入了深思,如何才能引领学生走出习作的困境呢？ 相似文献
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作文教学是语文教学的重要组成部分,学生的作文水平反映了其语文素养。许多学生在习作时总是抓耳挠腮,一脸的无奈,既使硬着头皮去写,写出来的作文也是无血无肉,缺少真情实感。面对如此现状,作为一名任教多年的教师,笔者不禁陷入了深思,如何才能引领学生走出习作的困境呢?一、有备无患在课前一般小学习作课一周安排两节,一学期大多数教师只是让学生写八九篇作文,这种训练很难保证习作教学的成效。再者,学生的知识面窄,只顾学习书本知识,不去留意、关注身 相似文献
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Objective: To investigate the infection of human embryo fibroblast cell line HF cells by CMV as well as the effects of CMV on β-actin mRNA and microfilaments. Methods: HF cells shape was observed after the infection of CMV. RT-PCR assay was used to detect the mRNA expression of CMV immediate early (IE) gene, β-actin and GAPDH genes of HF cells infected by CMV. CMV particles and cell microfilaments were detected with electron microscope. Results: Shape of HF cell changed after the infection by CMV. HF cells infected by CMV could express IE mRNA and the expression of β-actin mRNA decreased in a time- and titer-dependent manner compared with the uninfected HF cells whose expression of GAPDH mRNA did not change much. CMV particles were found with electron microscope in the cells. Microfilaments were ruptured and shortened after the infection of CMV. Conclusion: CMV can not only infect human embryo fibroblast cells line HF cells and replicate in the cells, but can also affect the expression of β-actin mRNA and the microfilaments. 相似文献
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