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Enhancing rice resistance to fungal pathogens by transformation with cell wall degrading enzyme genes from Trichoderma atroviride 总被引：1，自引：0，他引：1
INTRODUCTION Rice sheath blight (Rhizoctonia solani) and rice blast (Magnaporthe grisea) are two serious plantdiseases in China. Rice cultivars with high level and durable resistance to these two pathogens are noteasy to obtain by traditional breeding methods due to the lack of germplasm resistant to R. solani and the high genetic diversity of M. grisea. Cell wall degrading enzyme (CWDE) genes from biocontrol fungi belonging to the genus Trichoderma have been demonstrated to encode … 相似文献
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HARMAN BURCK EDWARD JACOBS RICHARD SAUBER WINIFRED STONE WILLIAM THOMSON 《Counselor Education & Supervision》1973,12(3):162-171
This article describes and evaluates an innovative way to teach a counseling theories and practices course to beginning counseling students. Cognitive and affective learning processes, continuous and systematic feedback, small group dynamics, and a cross-sectional approach to teaching theoretical concepts and techniques are described. This approach was implemented by using the following instructional format: didactic, demonstration, task simulation, and self-exploration. Evaluation included self—peer, student-staff, and staff-student evaluations and used measures obtained from six instrumented indices. Results suggest that this approach might be a useful alternative to teaching the traditional didactic course in theories and practices of counseling. 相似文献
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What is the responsibility of counselor education programs in more efficiently preparing students in the area of career guidance and development? This article attempts to explain one department's efforts in this important area. It explains the rationale, development, and implementation of a subspeciality, Career Development Specialist (CDS), in a counselor education program. Covered are areas such as historical/conceptual review of the CDS, competencies and courses, recruitment of students, and where graduates have located jobs. Finally, the implications for counselor education are discussed. 相似文献
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Three genes encoding for fungal cell wall degrading enzymes (CWDEs), ech42, nag70 and gluc78 from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants. More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance to Magnaporthe grisea was found in all kinds of regenerated plants including that with single gluc78. A few lines expressing either ech42 or nag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level. This is the first report of multiple of expression of genes encoding CWDEs from Trichoderma atroviride that result in resistance to blast and sheath blight in rice. 相似文献
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